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The map label for this gene is lcfA [H]

Identifier: 94312996

GI number: 94312996

Start: 632826

End: 634565

Strand: Reverse

Name: lcfA [H]

Synonym: Rmet_4068

Alternate gene names: 94312996

Gene position: 634565-632826 (Counterclockwise)

Preceding gene: 94312997

Following gene: 94312995

Centisome position: 24.59

GC content: 64.94

Gene sequence:

>1740_bases
ATGGAAGACGCAGCTTTCTTCGCAGATCTTGAAGCGCGGCATCGCCGCATCTGGCCCGCAGGCCTGCCCACCTCGCCGCA
GTATCCACACGGCGAGATCAGCCTTGGCGAGACCCTGCGGGCATGGGCGCGCATCCAGCCTGAGCGGCCCGCGCTGATCT
ACTACGGCAGCGAGATCACCTTCGCCGAACTCGACGACCTGAGCGAGCGCTGCGCGGAACTGCTGCGGGGCCACGGCATC
GGCCCCGGCAATCGCGTGGCCGTTCTACTGGGCAACTGTCCGCAGTTCCATATCGTGTTCTACGCGATTCTCAAGCTTGG
CGCCGTCTACGTGCCCGTGAATCCACTGTTCAAGGAACACGAACTGGCCTACGAGCTGAACGATGCCGGTGCGACCATGG
TGATCGCGCTCGACACGCTCGTGCCGATGCTGATGACGGTGAAGCCGCAAACCAGCGTGGAAACGGTCTTCAGCACATCG
GCCGGCGAATTCCTGCCCGCGCATCCCACGCTGCCCGTTCCGGCTGGACTCGACGCCGCGCCAGTGGCGCACGCGGGCAC
GATCGACCTGATGGCGGCCATCCAGACATGCACGGGCAACGTGCGCGACGTGAGCGTGGATCTCGATGCATTGGCCGCGC
TGAACTACACGGGTGGTACCACCGGTATGCCCAAGGGCTGCATGCATACCCAGCGCGACATGATCTACACGGCGGCCGCC
ACGCTGGCGGTCACCAGCGGGCGCGAAGACCGGGATGCAACGCTGCCGTCGTCCGACGTGATGCTGAACTTCCTGCCGAT
GTTCTGGATCGCCGGCGAGAACCTCGGATTGATCTATCCGATCTTCAGCGGCGCCACGCTGGTGCTGCTGGCGCGCTGGG
ACCCACTCGCCGTCATGTCTGCCGTGGACCGCTACCGCGTCAATCGCACGTTCATGGTGGTCGACAACCTTGTGGAACTG
CTGCAGCACCCCGAACTTGCGCGGCACGACATGCGCTCGCTGCAACATACGCGCGTGGCATCGTTCATCCGCAAGCTGAC
CGTGGACCTGCGCCGCCAGTGGCACGCGCTGACCGGCAGCGTCGTGGCCGAAGGCGCCTGGGGCATGACGGAGACGCACA
CGAGCGACACCTTCACCACGGGGATGCAGACAGACGACATGGACCTGCACGGCCGGCCAGTCTTCGTCGGTCTGCCAGTG
CCGGGCACGCGTATCAAGATCTGCGACTTCGAAACCGGCGTGACGCTGCCCGTTGGCGAGGAGGGCGAGATTGTCGTCAC
CACGCCTTCGCTGCTCAAGGGCTATTGGGGCCGCCCCGACGCCAATGCGGAGGTGTTCCGGGACGGCTGGTTCAGGACAG
GCGATATCGGCGCCTATGACGATGCGGGCTACCTGCACTTCCTTGGACGGCGCAAGGAGATGATCAAGGTGCGCGGCATG
AGCGTATTCCCGTCAGAGCTGGAAGTCCTGCTCGGACGTCATGCCGCCGTACTCGGGTCCGCCGTGGTCGCACGTCCCGA
TCGGGACAAGGGCCAGGTGCCGGTGGCATTCATCCGCCTGCGCCCCGGGCATGACGATGTGTCGGCCGAGGCGCTGACGG
CATGGTGCCGCGAGCATATGGCGACCTACAAGGTGCCCGAGATCCGCGTGGTGGACGAGTTGCCGATGACGGCAACGGGC
AAGGTCAAGAAGGGGGAACTGCAGAAACAGTTCGAGCAGGACGAACAAGAGGTGGGCTGA

Upstream 100 bases:

>100_bases
ACGTTACGTGGATGCGTGGTAGCCGATGCAGTGACTGAACTGGCCCCCTGACTCCGCTCCCGCCATGCTGGAGCGCGGAG
CAAATACTCCTGGAGACACG

Downstream 100 bases:

>100_bases
CAGCCCGCCACCGCGCCCCGACACGACCAAGAGACCATTGGAGACAACGATGAACACCCAGCGACGCCACTTGTTGAAGC
TCTCCGCCGCGATGCTCGGC

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MEDAAFFADLEARHRRIWPAGLPTSPQYPHGEISLGETLRAWARIQPERPALIYYGSEITFAELDDLSERCAELLRGHGI
GPGNRVAVLLGNCPQFHIVFYAILKLGAVYVPVNPLFKEHELAYELNDAGATMVIALDTLVPMLMTVKPQTSVETVFSTS
AGEFLPAHPTLPVPAGLDAAPVAHAGTIDLMAAIQTCTGNVRDVSVDLDALAALNYTGGTTGMPKGCMHTQRDMIYTAAA
TLAVTSGREDRDATLPSSDVMLNFLPMFWIAGENLGLIYPIFSGATLVLLARWDPLAVMSAVDRYRVNRTFMVVDNLVEL
LQHPELARHDMRSLQHTRVASFIRKLTVDLRRQWHALTGSVVAEGAWGMTETHTSDTFTTGMQTDDMDLHGRPVFVGLPV
PGTRIKICDFETGVTLPVGEEGEIVVTTPSLLKGYWGRPDANAEVFRDGWFRTGDIGAYDDAGYLHFLGRRKEMIKVRGM
SVFPSELEVLLGRHAAVLGSAVVARPDRDKGQVPVAFIRLRPGHDDVSAEALTAWCREHMATYKVPEIRVVDELPMTATG
KVKKGELQKQFEQDEQEVG

Sequences:

>Translated_579_residues
MEDAAFFADLEARHRRIWPAGLPTSPQYPHGEISLGETLRAWARIQPERPALIYYGSEITFAELDDLSERCAELLRGHGI
GPGNRVAVLLGNCPQFHIVFYAILKLGAVYVPVNPLFKEHELAYELNDAGATMVIALDTLVPMLMTVKPQTSVETVFSTS
AGEFLPAHPTLPVPAGLDAAPVAHAGTIDLMAAIQTCTGNVRDVSVDLDALAALNYTGGTTGMPKGCMHTQRDMIYTAAA
TLAVTSGREDRDATLPSSDVMLNFLPMFWIAGENLGLIYPIFSGATLVLLARWDPLAVMSAVDRYRVNRTFMVVDNLVEL
LQHPELARHDMRSLQHTRVASFIRKLTVDLRRQWHALTGSVVAEGAWGMTETHTSDTFTTGMQTDDMDLHGRPVFVGLPV
PGTRIKICDFETGVTLPVGEEGEIVVTTPSLLKGYWGRPDANAEVFRDGWFRTGDIGAYDDAGYLHFLGRRKEMIKVRGM
SVFPSELEVLLGRHAAVLGSAVVARPDRDKGQVPVAFIRLRPGHDDVSAEALTAWCREHMATYKVPEIRVVDELPMTATG
KVKKGELQKQFEQDEQEVG
>Mature_579_residues
MEDAAFFADLEARHRRIWPAGLPTSPQYPHGEISLGETLRAWARIQPERPALIYYGSEITFAELDDLSERCAELLRGHGI
GPGNRVAVLLGNCPQFHIVFYAILKLGAVYVPVNPLFKEHELAYELNDAGATMVIALDTLVPMLMTVKPQTSVETVFSTS
AGEFLPAHPTLPVPAGLDAAPVAHAGTIDLMAAIQTCTGNVRDVSVDLDALAALNYTGGTTGMPKGCMHTQRDMIYTAAA
TLAVTSGREDRDATLPSSDVMLNFLPMFWIAGENLGLIYPIFSGATLVLLARWDPLAVMSAVDRYRVNRTFMVVDNLVEL
LQHPELARHDMRSLQHTRVASFIRKLTVDLRRQWHALTGSVVAEGAWGMTETHTSDTFTTGMQTDDMDLHGRPVFVGLPV
PGTRIKICDFETGVTLPVGEEGEIVVTTPSLLKGYWGRPDANAEVFRDGWFRTGDIGAYDDAGYLHFLGRRKEMIKVRGM
SVFPSELEVLLGRHAAVLGSAVVARPDRDKGQVPVAFIRLRPGHDDVSAEALTAWCREHMATYKVPEIRVVDELPMTATG
KVKKGELQKQFEQDEQEVG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=557, Percent_Identity=29.0843806104129, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI42544132, Length=543, Percent_Identity=27.8084714548803, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI115511026, Length=556, Percent_Identity=26.6187050359712, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI58082049, Length=574, Percent_Identity=26.3066202090592, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI156151445, Length=557, Percent_Identity=24.0574506283662, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI157311624, Length=574, Percent_Identity=25.9581881533101, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI157311622, Length=574, Percent_Identity=25.9581881533101, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI122937307, Length=543, Percent_Identity=27.4401473296501, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI187761345, Length=572, Percent_Identity=27.2727272727273, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI187761343, Length=572, Percent_Identity=27.2727272727273, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=24.7349823321555, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI13375727, Length=540, Percent_Identity=23.3333333333333, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI57165410, Length=144, Percent_Identity=37.5, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI57165412, Length=144, Percent_Identity=37.5, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=576, Percent_Identity=29.3402777777778, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI145693145, Length=551, Percent_Identity=27.7676950998185, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI221142682, Length=528, Percent_Identity=25.9469696969697, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=27.134724857685, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786801, Length=475, Percent_Identity=25.0526315789474, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1786529, Length=560, Percent_Identity=25.3571428571429, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=517, Percent_Identity=28.4332688588008, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17538037, Length=511, Percent_Identity=26.8101761252446, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=551, Percent_Identity=25.4083484573503, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=599, Percent_Identity=24.5409015025042, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=599, Percent_Identity=24.5409015025042, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=544, Percent_Identity=25, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=591, Percent_Identity=24.8730964467005, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=582, Percent_Identity=24.3986254295533, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=559, Percent_Identity=25.4025044722719, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=465, Percent_Identity=27.0967741935484, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71985884, Length=579, Percent_Identity=22.4525043177893, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=353, Percent_Identity=27.7620396600567, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983001, Length=594, Percent_Identity=24.5791245791246, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=594, Percent_Identity=24.5791245791246, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=572, Percent_Identity=24.3006993006993, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=516, Percent_Identity=25.5813953488372, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=584, Percent_Identity=23.8013698630137, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18859661, Length=541, Percent_Identity=28.0961182994455, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21355181, Length=566, Percent_Identity=24.0282685512367, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24581924, Length=549, Percent_Identity=24.0437158469945, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648260, Length=566, Percent_Identity=22.791519434629, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648257, Length=375, Percent_Identity=25.3333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356947, Length=583, Percent_Identity=22.4699828473413, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653035, Length=374, Percent_Identity=24.5989304812834, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24667955, Length=554, Percent_Identity=24.5487364620939, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI62472339, Length=554, Percent_Identity=24.5487364620939, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI281365686, Length=372, Percent_Identity=20.9677419354839, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161076582, Length=394, Percent_Identity=24.1116751269036, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=25.2717391304348, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922652, Length=569, Percent_Identity=23.1985940246046, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356441, Length=570, Percent_Identity=21.4035087719298, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358303, Length=571, Percent_Identity=19.614711033275, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355225, Length=547, Percent_Identity=24.8628884826325, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24667959, Length=375, Percent_Identity=25.0666666666667, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648253, Length=565, Percent_Identity=20.7079646017699, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648255, Length=572, Percent_Identity=20.4545454545455, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 63613; Mature: 63613

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDAAFFADLEARHRRIWPAGLPTSPQYPHGEISLGETLRAWARIQPERPALIYYGSEIT
CCCCHHHHHHHHHHCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEEE
FAELDDLSERCAELLRGHGIGPGNRVAVLLGNCPQFHIVFYAILKLGAVYVPVNPLFKEH
EHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEECCCCCCCCC
ELAYELNDAGATMVIALDTLVPMLMTVKPQTSVETVFSTSAGEFLPAHPTLPVPAGLDAA
CEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PVAHAGTIDLMAAIQTCTGNVRDVSVDLDALAALNYTGGTTGMPKGCMHTQRDMIYTAAA
CCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHH
TLAVTSGREDRDATLPSSDVMLNFLPMFWIAGENLGLIYPIFSGATLVLLARWDPLAVMS
EEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEEECCCHHHHHH
AVDRYRVNRTFMVVDNLVELLQHPELARHDMRSLQHTRVASFIRKLTVDLRRQWHALTGS
HHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VVAEGAWGMTETHTSDTFTTGMQTDDMDLHGRPVFVGLPVPGTRIKICDFETGVTLPVGE
HHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEECCC
EGEIVVTTPSLLKGYWGRPDANAEVFRDGWFRTGDIGAYDDAGYLHFLGRRKEMIKVRGM
CCCEEEECHHHHHCCCCCCCCCCHHHHCCCEECCCCCCCCCCCHHHHHHCCHHEEEECCC
SVFPSELEVLLGRHAAVLGSAVVARPDRDKGQVPVAFIRLRPGHDDVSAEALTAWCREHM
EECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ATYKVPEIRVVDELPMTATGKVKKGELQKQFEQDEQEVG
HHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEDAAFFADLEARHRRIWPAGLPTSPQYPHGEISLGETLRAWARIQPERPALIYYGSEIT
CCCCHHHHHHHHHHCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEEE
FAELDDLSERCAELLRGHGIGPGNRVAVLLGNCPQFHIVFYAILKLGAVYVPVNPLFKEH
EHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEECCCCCCCCC
ELAYELNDAGATMVIALDTLVPMLMTVKPQTSVETVFSTSAGEFLPAHPTLPVPAGLDAA
CEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PVAHAGTIDLMAAIQTCTGNVRDVSVDLDALAALNYTGGTTGMPKGCMHTQRDMIYTAAA
CCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHH
TLAVTSGREDRDATLPSSDVMLNFLPMFWIAGENLGLIYPIFSGATLVLLARWDPLAVMS
EEEEECCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEEECCCHHHHHH
AVDRYRVNRTFMVVDNLVELLQHPELARHDMRSLQHTRVASFIRKLTVDLRRQWHALTGS
HHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VVAEGAWGMTETHTSDTFTTGMQTDDMDLHGRPVFVGLPVPGTRIKICDFETGVTLPVGE
HHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEECCC
EGEIVVTTPSLLKGYWGRPDANAEVFRDGWFRTGDIGAYDDAGYLHFLGRRKEMIKVRGM
CCCEEEECHHHHHCCCCCCCCCCHHHHCCCEECCCCCCCCCCCHHHHHHCCHHEEEECCC
SVFPSELEVLLGRHAAVLGSAVVARPDRDKGQVPVAFIRLRPGHDDVSAEALTAWCREHM
EECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ATYKVPEIRVVDELPMTATGKVKKGELQKQFEQDEQEVG
HHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]