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The map label for this gene is kefA [H]

Identifier: 94312957

GI number: 94312957

Start: 597444

End: 599942

Strand: Reverse

Name: kefA [H]

Synonym: Rmet_4029

Alternate gene names: 94312957

Gene position: 599942-597444 (Counterclockwise)

Preceding gene: 94312960

Following gene: 94312954

Centisome position: 23.25

GC content: 64.91

Gene sequence:

>2499_bases
TTGTTCATCCTGGCGCGTCGCGCCCGGCACTGCCTGCCGGGACTGGCAAACCCGGAAAATTTCCACGAAGTGCAATTCGC
CATGAGCAAGATTTCCAGGCTCGCCTTGAGCCTTGTATTGGCCGCACTGTTTCATGTCGCCGCCCCCGCGCTTGCCGCGG
AAAGCGCCTCGGACGCGGAGGCCCAGCAGATTCCCGTGCTGACGCACGCGGAGGCCGTTGCCGAACTCAAGCGCCTGCAG
ACCGCGCAGGACCAGATCAAGCAGCAGGCCTCCAGCGCCTTGGGGCGCACCAAAATCAATGGACTCGACGACGACCTCAA
GCAACTGGCCACCGATGTCGACAAGCTCGCTGCATCACTGACGCCGCAACGCGCGCAGCTGCAATCCCAGCTTGATGTGC
TGGGCCCGCCGCCCGAAACCGGCAAGGCAAAGGAAGCCCCCGCCGTGGTCGCGCAGCGCGCCGAACTCACCGCGCGCAAG
ACGCAGCTCGACGACGAACTGAAGCAGGCGGCCAACAGCAAGGACAACATCGCGCACCTGCAGGAACAGCTGGCCAAACT
GCAACGCACGCAGATGAAGGACCAGCTTGCTTTGCGGTCCGAGAGCATCCTGAATCCCCAGTTCTGGAAGCCGCTCTTCG
GGCGCGCGCCCGAGGACCCGGAGCAGCCCGGCGCCTTCACCGACGAGATCGTCCCAATGTTCGAGCACGCCTGGGCGTCT
GGGCAACGGGCGACCACGGTCATCCTGCTCGTGATCGCACTGGCCGCGTGGACACTGGGGCGTCGTCTGCTCGAACGCGG
CGCGGCGTGGCTCTGCCTGACGCAGTTGCCCGAAGCTCGCATCCGCCGTAGCGCGCTGGCGTTCGCCACAACACTGGCCA
CGGTGATCGCCACGGCCACCGGCGTGCGCATTGCATTCGCGGCGTTCACCGGCGGAGAGGACGTCCCGCCCGAAATTCAG
GACCTGCTGAACGAGATCGCCAAGCTGGCAATGACCAGCGCACTGATCGCGGGCCTGGGCCGCGCCCTGCTCTGCACGCG
GCATCCTTCATGGCGGCTGCCTGCGCTGGCCGACCGGCTGGCGCTCGCCATGAAGCCGTTCCCGACGGTGCTGGCAGGCT
TGCTCCTGCTGGCCGGCACGCTTGAGCAGTTCAACCGCATGGCCGATACGAGCCTGCCGGTCACGCTGCTCGGCAGAGGC
GTTGTGTCCTTGGTGGTGGTGTTGACGATTGGCGCCGCACTGCTGCGTGCCAGTCGCGTGCGCGCCGAACTGCTCGCAGC
CGGAGAACAGCCCGAAGCCCGCGCCACGCTCGTCGGATTGATCCACGTCGGCGTCACGCTGACCGTGGCGATCTCGTTCG
GCGCGCTGCTGATCGGCTACATCAGCATCGCGCGCTTCCTGACCTACGAACTGGTCTGGTTCGATATCGTGCTGTGCAGC
CTCTACCTGCTCACGCAACTGACGCGCGACATCTGCGAGGCCACGTTCTCCACGCAGTACGGCGGCGGACGCGTCATCAA
GCAACTGTTCGGCGCAGGCGATACCCAGCTCGAACAGGCCTCCACGGTCTTTTCCGGTCTCGGCTCCAGCGTGCTGCTTC
TGCTGGCCGTGCTGGCGCTCCTCACCGGCGGCTTCGGCACGACACCCGCCGATCTCTTCAACAGCCTGCTGGCCATGCTC
GGCGGCGAGAAGCTACGCGCGCTGAACATCATGCCGGACCGCATCATGAACGCGGTGATCGCGCTTGTGGTCGGCATCTG
GCTGCTACGCTCGGTACGCCGATGGCTCGATGCGGAACTGCTGCCGAAGCTTTGCTCGGAGTCCGGGCTGCGTGCGTCGC
TGATCACGCTGTTCAGCAATATCGGCTATGTGCTGCTGGTGCTGCTGGTGCTCTCGTTGCTGGGCGTCAAATGGGAAAAC
CTGGCGTGGATCGTCAGCGCGCTGTCGGTCGGTATCGGCTTCGGCTTGCAGGAGATCGTCAAGAACTTCGTCTCCGGCCT
GATCCTGCTGACCGAGCGCCCGGTCAAGGTAGGCGACATGGTCAGCCTGGCCGGCGTCGAAGGCGATATCCGTCGCATCA
ACGTACGCGCGACGGAAATCCAGCTGGCCGACCGCTCGACCGTGATCGTGCCGAACTCGCAGTTGATCTCGCAGAACCTG
CGTAACGTCACGATGCACAACAACACGCAAGGCGTAGCCACGCTGCAGTTGACGTTCCCGCTGAACACCGACCCCGAAGC
CGTGCGCGACCTGCTGCTCGACGTCTACCGCCAGAACGAAGCGATCCTCGACATCCCCGCGCCATCGGTGACGTTCAGCC
AGTTGGCGCCGAACGGCATCACGCTGAGCGTCACCGGCTATGTGAACAGCCCCCGCATCGCTGGTGGCACCAAGAGCGAC
CTGCTGTTCGAGATCCTCAAGCGGCTACGCGCCGAGGAGATTCCACTGTCGGCGCCACAAACGCTGCGGCTGGAAAATAT
GGAGGCATTGGGGACGTAA

Upstream 100 bases:

>100_bases
CCCAGACTCAGTCTCCTCCGCCGGGCGGCATCCCATCCGCATGTCAGCCCGCACTCGTTGAAAGTGGCGGCCCAGCGCAC
CGTGCGCGGCTGCGCTTTCG

Downstream 100 bases:

>100_bases
ATAGGGGTGTAGATCAGGGACGTAGATTGGGACGAGTTGACTCCCCGTCACCCCATCACCACGTCACCCCATTGCTCCTT
CGTGCCCAGTCCAATAATCG

Product: putative mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 832; Mature: 832

Protein sequence:

>832_residues
MFILARRARHCLPGLANPENFHEVQFAMSKISRLALSLVLAALFHVAAPALAAESASDAEAQQIPVLTHAEAVAELKRLQ
TAQDQIKQQASSALGRTKINGLDDDLKQLATDVDKLAASLTPQRAQLQSQLDVLGPPPETGKAKEAPAVVAQRAELTARK
TQLDDELKQAANSKDNIAHLQEQLAKLQRTQMKDQLALRSESILNPQFWKPLFGRAPEDPEQPGAFTDEIVPMFEHAWAS
GQRATTVILLVIALAAWTLGRRLLERGAAWLCLTQLPEARIRRSALAFATTLATVIATATGVRIAFAAFTGGEDVPPEIQ
DLLNEIAKLAMTSALIAGLGRALLCTRHPSWRLPALADRLALAMKPFPTVLAGLLLLAGTLEQFNRMADTSLPVTLLGRG
VVSLVVVLTIGAALLRASRVRAELLAAGEQPEARATLVGLIHVGVTLTVAISFGALLIGYISIARFLTYELVWFDIVLCS
LYLLTQLTRDICEATFSTQYGGGRVIKQLFGAGDTQLEQASTVFSGLGSSVLLLLAVLALLTGGFGTTPADLFNSLLAML
GGEKLRALNIMPDRIMNAVIALVVGIWLLRSVRRWLDAELLPKLCSESGLRASLITLFSNIGYVLLVLLVLSLLGVKWEN
LAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSLAGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNL
RNVTMHNNTQGVATLQLTFPLNTDPEAVRDLLLDVYRQNEAILDIPAPSVTFSQLAPNGITLSVTGYVNSPRIAGGTKSD
LLFEILKRLRAEEIPLSAPQTLRLENMEALGT

Sequences:

>Translated_832_residues
MFILARRARHCLPGLANPENFHEVQFAMSKISRLALSLVLAALFHVAAPALAAESASDAEAQQIPVLTHAEAVAELKRLQ
TAQDQIKQQASSALGRTKINGLDDDLKQLATDVDKLAASLTPQRAQLQSQLDVLGPPPETGKAKEAPAVVAQRAELTARK
TQLDDELKQAANSKDNIAHLQEQLAKLQRTQMKDQLALRSESILNPQFWKPLFGRAPEDPEQPGAFTDEIVPMFEHAWAS
GQRATTVILLVIALAAWTLGRRLLERGAAWLCLTQLPEARIRRSALAFATTLATVIATATGVRIAFAAFTGGEDVPPEIQ
DLLNEIAKLAMTSALIAGLGRALLCTRHPSWRLPALADRLALAMKPFPTVLAGLLLLAGTLEQFNRMADTSLPVTLLGRG
VVSLVVVLTIGAALLRASRVRAELLAAGEQPEARATLVGLIHVGVTLTVAISFGALLIGYISIARFLTYELVWFDIVLCS
LYLLTQLTRDICEATFSTQYGGGRVIKQLFGAGDTQLEQASTVFSGLGSSVLLLLAVLALLTGGFGTTPADLFNSLLAML
GGEKLRALNIMPDRIMNAVIALVVGIWLLRSVRRWLDAELLPKLCSESGLRASLITLFSNIGYVLLVLLVLSLLGVKWEN
LAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSLAGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNL
RNVTMHNNTQGVATLQLTFPLNTDPEAVRDLLLDVYRQNEAILDIPAPSVTFSQLAPNGITLSVTGYVNSPRIAGGTKSD
LLFEILKRLRAEEIPLSAPQTLRLENMEALGT
>Mature_832_residues
MFILARRARHCLPGLANPENFHEVQFAMSKISRLALSLVLAALFHVAAPALAAESASDAEAQQIPVLTHAEAVAELKRLQ
TAQDQIKQQASSALGRTKINGLDDDLKQLATDVDKLAASLTPQRAQLQSQLDVLGPPPETGKAKEAPAVVAQRAELTARK
TQLDDELKQAANSKDNIAHLQEQLAKLQRTQMKDQLALRSESILNPQFWKPLFGRAPEDPEQPGAFTDEIVPMFEHAWAS
GQRATTVILLVIALAAWTLGRRLLERGAAWLCLTQLPEARIRRSALAFATTLATVIATATGVRIAFAAFTGGEDVPPEIQ
DLLNEIAKLAMTSALIAGLGRALLCTRHPSWRLPALADRLALAMKPFPTVLAGLLLLAGTLEQFNRMADTSLPVTLLGRG
VVSLVVVLTIGAALLRASRVRAELLAAGEQPEARATLVGLIHVGVTLTVAISFGALLIGYISIARFLTYELVWFDIVLCS
LYLLTQLTRDICEATFSTQYGGGRVIKQLFGAGDTQLEQASTVFSGLGSSVLLLLAVLALLTGGFGTTPADLFNSLLAML
GGEKLRALNIMPDRIMNAVIALVVGIWLLRSVRRWLDAELLPKLCSESGLRASLITLFSNIGYVLLVLLVLSLLGVKWEN
LAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSLAGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNL
RNVTMHNNTQGVATLQLTFPLNTDPEAVRDLLLDVYRQNEAILDIPAPSVTFSQLAPNGITLSVTGYVNSPRIAGGTKSD
LLFEILKRLRAEEIPLSAPQTLRLENMEALGT

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=252, Percent_Identity=32.5396825396825, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI2367355, Length=248, Percent_Identity=31.0483870967742, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1789291, Length=182, Percent_Identity=22.5274725274725, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 89754; Mature: 89754

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFILARRARHCLPGLANPENFHEVQFAMSKISRLALSLVLAALFHVAAPALAAESASDAE
CEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AQQIPVLTHAEAVAELKRLQTAQDQIKQQASSALGRTKINGLDDDLKQLATDVDKLAASL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
TPQRAQLQSQLDVLGPPPETGKAKEAPAVVAQRAELTARKTQLDDELKQAANSKDNIAHL
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QEQLAKLQRTQMKDQLALRSESILNPQFWKPLFGRAPEDPEQPGAFTDEIVPMFEHAWAS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
GQRATTVILLVIALAAWTLGRRLLERGAAWLCLTQLPEARIRRSALAFATTLATVIATAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GVRIAFAAFTGGEDVPPEIQDLLNEIAKLAMTSALIAGLGRALLCTRHPSWRLPALADRL
CHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCHHHHHHH
ALAMKPFPTVLAGLLLLAGTLEQFNRMADTSLPVTLLGRGVVSLVVVLTIGAALLRASRV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELLAAGEQPEARATLVGLIHVGVTLTVAISFGALLIGYISIARFLTYELVWFDIVLCS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLLTQLTRDICEATFSTQYGGGRVIKQLFGAGDTQLEQASTVFSGLGSSVLLLLAVLAL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LTGGFGTTPADLFNSLLAMLGGEKLRALNIMPDRIMNAVIALVVGIWLLRSVRRWLDAEL
HHCCCCCCHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LPKLCSESGLRASLITLFSNIGYVLLVLLVLSLLGVKWENLAWIVSALSVGIGFGLQEIV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
KNFVSGLILLTERPVKVGDMVSLAGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNL
HHHHHHEEEECCCCCCCCCHHHHCCCCCCCEEEEEEEEEEEEECCCEEECCCHHHHHHHH
RNVTMHNNTQGVATLQLTFPLNTDPEAVRDLLLDVYRQNEAILDIPAPSVTFSQLAPNGI
HHCEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCE
TLSVTGYVNSPRIAGGTKSDLLFEILKRLRAEEIPLSAPQTLRLENMEALGT
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MFILARRARHCLPGLANPENFHEVQFAMSKISRLALSLVLAALFHVAAPALAAESASDAE
CEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AQQIPVLTHAEAVAELKRLQTAQDQIKQQASSALGRTKINGLDDDLKQLATDVDKLAASL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
TPQRAQLQSQLDVLGPPPETGKAKEAPAVVAQRAELTARKTQLDDELKQAANSKDNIAHL
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QEQLAKLQRTQMKDQLALRSESILNPQFWKPLFGRAPEDPEQPGAFTDEIVPMFEHAWAS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
GQRATTVILLVIALAAWTLGRRLLERGAAWLCLTQLPEARIRRSALAFATTLATVIATAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GVRIAFAAFTGGEDVPPEIQDLLNEIAKLAMTSALIAGLGRALLCTRHPSWRLPALADRL
CHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCHHHHHHH
ALAMKPFPTVLAGLLLLAGTLEQFNRMADTSLPVTLLGRGVVSLVVVLTIGAALLRASRV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELLAAGEQPEARATLVGLIHVGVTLTVAISFGALLIGYISIARFLTYELVWFDIVLCS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLLTQLTRDICEATFSTQYGGGRVIKQLFGAGDTQLEQASTVFSGLGSSVLLLLAVLAL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LTGGFGTTPADLFNSLLAMLGGEKLRALNIMPDRIMNAVIALVVGIWLLRSVRRWLDAEL
HHCCCCCCHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LPKLCSESGLRASLITLFSNIGYVLLVLLVLSLLGVKWENLAWIVSALSVGIGFGLQEIV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
KNFVSGLILLTERPVKVGDMVSLAGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNL
HHHHHHEEEECCCCCCCCCHHHHCCCCCCCEEEEEEEEEEEEECCCEEECCCHHHHHHHH
RNVTMHNNTQGVATLQLTFPLNTDPEAVRDLLLDVYRQNEAILDIPAPSVTFSQLAPNGI
HHCEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCE
TLSVTGYVNSPRIAGGTKSDLLFEILKRLRAEEIPLSAPQTLRLENMEALGT
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]