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The map label for this gene is livM4 [H]

Identifier: 94312907

GI number: 94312907

Start: 545391

End: 546419

Strand: Reverse

Name: livM4 [H]

Synonym: Rmet_3979

Alternate gene names: 94312907

Gene position: 546419-545391 (Counterclockwise)

Preceding gene: 94312908

Following gene: 94312906

Centisome position: 21.18

GC content: 63.27

Gene sequence:

>1029_bases
ATGAAATCAATGCAAGGCAAAATCGGCTGGACGCTGTTGTTCGTCGCGGCCATCGTGTTCCCGCTGGCCGTCCCCAACAG
CTACTACCTGACGGTGATGACGCTGGCCTTCATCATGGCCATCGCCACGCTTGGGCTGAACCTGCTGACCGGATACACGG
GCCAGCTCAATCTCGCGCATGGCGGCTTCATGGCCATCGGCGCCTACACGCTGGGCATCCTGACCGTCGATCATCAGATG
CCGTACTGGCTCGCGTTCGCGTTGTCCGGCGTGGTCTGCATGGTGCTGGGTTATGTCGTCGGCACGGTGTCGCTGCGCCT
GAAAGGGCACTACTTCTCGATCTTCACGATGTGCGTAGGCTACATCATCTATCTGTTGATCGAGAAATGGGAAAGCCTGA
CCCATGGTGCGGTAGGCCTGATCGGCATCCCGGTCCCGGCTTCGATCGGTCCGATCGCCTTCGACAGCGTGCAGGCGCAG
TACTACCTGGTGCTGGTCTTCCTGGCCCTGGGCACGTTCGTCATGCATCGCATCGTCAGCTCACTGGTCGGCCGCAGCTT
CATGGCCGTGCGTAACAGCGATGCTCTCGCGGAAGCGCTCGGCATCAACCTGATGCGCACCAAGGTCTTGTCGTTCGTGC
TCTCCGTGGGCTACGCCGGATTCGCCGGCGCGCTCTATGCCGGCCAGGTACGCTTCCTGGGTCCGGACATCGCCCGCACC
GACATCACGTTCGACATGGTCATGGCGATGCTGGTCGGCGGCACGGGCACGCTACTCGGTCCGCTGCTTGGTTCGCTGCT
GGTACCGTGGGTCACGCAGACCCTGCAGTTCCTGCAGGACTACCGGATGCTCGTGTTCGGTCCGGTGCTGATCCTGCTGA
TCATCTTCGTGCCGGATGGCATCGTCGGTTCGTTCCTGAAGCGGCAAGTCCGCCGAGCCGCCGCCGCGCGCCGTGGGCAG
ATGGCCGCCCCGCGCCCGACGCAAACCACGTCCGTCCCGACCACCCGCCCCGGAGCCGAGAATGCTTGA

Upstream 100 bases:

>100_bases
AAGGACATCATCGCCTTCCTGCTGCTTGTGATCATCCTGTCGGTGCGTCCGCAGGGCCTGTTCGCCGGCAAGACCGCCTG
ACAGGAACCAGGAGACCACC

Downstream 100 bases:

>100_bases
GATCCGCAACCTGACCAAGAAATTTGGCGGCCTGACCGCGGTACACGATGTGTCCGTAAAGTTCGAGACCGGCCATATCA
ACGCGATCATCGGTCCCAAT

Product: ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAHGGFMAIGAYTLGILTVDHQM
PYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVGYIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQ
YYLVLVFLALGTFVMHRIVSSLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIART
DITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDGIVGSFLKRQVRRAAAARRGQ
MAAPRPTQTTSVPTTRPGAENA

Sequences:

>Translated_342_residues
MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAHGGFMAIGAYTLGILTVDHQM
PYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVGYIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQ
YYLVLVFLALGTFVMHRIVSSLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIART
DITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDGIVGSFLKRQVRRAAAARRGQ
MAAPRPTQTTSVPTTRPGAENA
>Mature_342_residues
MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAHGGFMAIGAYTLGILTVDHQM
PYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVGYIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQ
YYLVLVFLALGTFVMHRIVSSLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIART
DITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDGIVGSFLKRQVRRAAAARRGQ
MAAPRPTQTTSVPTTRPGAENA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=317, Percent_Identity=29.0220820189274, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36832; Mature: 36832

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAH
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GGFMAIGAYTLGILTVDHQMPYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVG
CCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
YIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQYYLVLVFLALGTFVMHRIVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIART
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCHHHH
DITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
IVGSFLKRQVRRAAAARRGQMAAPRPTQTTSVPTTRPGAENA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAH
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GGFMAIGAYTLGILTVDHQMPYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVG
CCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
YIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQYYLVLVFLALGTFVMHRIVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIART
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEECCCCHHHH
DITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
IVGSFLKRQVRRAAAARRGQMAAPRPTQTTSVPTTRPGAENA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]