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The map label for this gene is proP [H]

Identifier: 94312878

GI number: 94312878

Start: 507040

End: 508350

Strand: Reverse

Name: proP [H]

Synonym: Rmet_3950

Alternate gene names: 94312878

Gene position: 508350-507040 (Counterclockwise)

Preceding gene: 291481501

Following gene: 94312876

Centisome position: 19.7

GC content: 65.22

Gene sequence:

>1311_bases
ATGTCCACCTCCAGCAGTACTGCCGGGGCTTCGGGTGCGCAAGGCGGCCATGCCGCTCGCCCGCTGACCGGTCAGGATTA
CAAGACGCTCGCGCTTGCCGCACTTGGCGGCGCGCTCGAGTTCTACGATTTCATTATTTTTGTTTTCTTCGCCAAGGTCA
TTGGCCAGTTATTCTTCCCGGCATCCGTGCCGGACTGGCTGCGCATGCTGCAGACCTTCGGCATCTTCGCGGCCGGCTAC
CTGGCACGCCCGCTGGGCGGCATCATCATGGCGCACTTCGGTGACCTGCTCGGCCGCAAGAAAATGTTTACGTTGTCGAT
TCTGCTGATGTCGGTACCGACGCTGCTGATGGGCCTTCTGCCGACCTACGCTTCGGTGGGCCTGCTGGCGCCGATGGCGC
TGCTGGTGCTGCGCATCCTGCAGGGTGCCGCCGTGGGCGGCGAAGTCCCCGGCGCATGGGTGTTCGTGTCCGAACACGTG
CCCCGGCGTCACGTCGGATATGCCTGCGGCACGCTGACTGCAGGTCTGACGGCCGGCATCCTGCTCGGCTCGCTGGTAGC
CACAGGCATCAACGCCGTATTCGATCCGCAGGAACTCGTTGACCATGGCTGGCGCGTGCCGTTCGTGCTGGGCGGCGTGT
TCGGTGTCGGCTCGATGTACCTGCGCCGCTGGCTCCATGAAACCCCGGTCTTCGCGGAACTGCAGCAACGCAAGGCGCTG
GCCGCCGAACTGCCGCTCAAGACGGTCGTGCGCGACCACCGCGGCGCCGTTGTCATCTCGATGCTGCTCACGTGGATGCT
GTCCGCTGGCATCGTGGTGGTGATCCTGCTGACGCCGACCTACCTCCAGACGCTGTACGGCTTCGACGCCCGCACCGCGC
TGGTGGCCAACAGCGCCGCAACGCTGTGCCTGTCGATCGGCTGCGTGGTGGCCGGCATCCTGGCTGACCGCATTGGCGCG
CGCCTGACACTTTCGATCGGTGGCGCACTGCTGGCTGCCACGGCCTGCGTGCTGTACACGACGATCGGCACCCGTCCCGA
CCTGCTGCTGCCGCTGTACGCCCTGGCCGGCTTCTTCGTCGGCACGATTGGCGCCGTGCCTTACGTGCTGGTGCACGCGT
TCCCAGCGCAAGTGCGGTTCTCCGGGCTGTCCTTCTCGTACAACGTCTCGTACGCGATCTTCGGCGGACTGACGCCGGTG
ATCGTCTCGCTGATGCTCAAGAATGATTCGCTGGCCCCGGCCCACTATGTGGTTGGCGTCTGCATCATGGGTATCGTGAC
GGCGCTGTTCGTACGCAAGCGGCACGCCTGA

Upstream 100 bases:

>100_bases
TCCGGTTCGCTCAGTTCGTGCTGTTATCCCTTCGGCCGTCCCCCTCCACGTCATCCGTGTTGCGCGCTTCGCATGTGAAT
GTATCCATGCGAGTCTTTTC

Downstream 100 bases:

>100_bases
TCGCGCTCGCCACGCTTGCCGCGCTGGCACAATCGAAAAGGGGGAAGGCTGACAGCCTTCCCCCTTTTTCATTTGGGCGC
GCTTCGGGCCCCGTCCTGCG

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTSSSTAGASGAQGGHAARPLTGQDYKTLALAALGGALEFYDFIIFVFFAKVIGQLFFPASVPDWLRMLQTFGIFAAGY
LARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLLPTYASVGLLAPMALLVLRILQGAAVGGEVPGAWVFVSEHV
PRRHVGYACGTLTAGLTAGILLGSLVATGINAVFDPQELVDHGWRVPFVLGGVFGVGSMYLRRWLHETPVFAELQQRKAL
AAELPLKTVVRDHRGAVVISMLLTWMLSAGIVVVILLTPTYLQTLYGFDARTALVANSAATLCLSIGCVVAGILADRIGA
RLTLSIGGALLAATACVLYTTIGTRPDLLLPLYALAGFFVGTIGAVPYVLVHAFPAQVRFSGLSFSYNVSYAIFGGLTPV
IVSLMLKNDSLAPAHYVVGVCIMGIVTALFVRKRHA

Sequences:

>Translated_436_residues
MSTSSSTAGASGAQGGHAARPLTGQDYKTLALAALGGALEFYDFIIFVFFAKVIGQLFFPASVPDWLRMLQTFGIFAAGY
LARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLLPTYASVGLLAPMALLVLRILQGAAVGGEVPGAWVFVSEHV
PRRHVGYACGTLTAGLTAGILLGSLVATGINAVFDPQELVDHGWRVPFVLGGVFGVGSMYLRRWLHETPVFAELQQRKAL
AAELPLKTVVRDHRGAVVISMLLTWMLSAGIVVVILLTPTYLQTLYGFDARTALVANSAATLCLSIGCVVAGILADRIGA
RLTLSIGGALLAATACVLYTTIGTRPDLLLPLYALAGFFVGTIGAVPYVLVHAFPAQVRFSGLSFSYNVSYAIFGGLTPV
IVSLMLKNDSLAPAHYVVGVCIMGIVTALFVRKRHA
>Mature_435_residues
STSSSTAGASGAQGGHAARPLTGQDYKTLALAALGGALEFYDFIIFVFFAKVIGQLFFPASVPDWLRMLQTFGIFAAGYL
ARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLLPTYASVGLLAPMALLVLRILQGAAVGGEVPGAWVFVSEHVP
RRHVGYACGTLTAGLTAGILLGSLVATGINAVFDPQELVDHGWRVPFVLGGVFGVGSMYLRRWLHETPVFAELQQRKALA
AELPLKTVVRDHRGAVVISMLLTWMLSAGIVVVILLTPTYLQTLYGFDARTALVANSAATLCLSIGCVVAGILADRIGAR
LTLSIGGALLAATACVLYTTIGTRPDLLLPLYALAGFFVGTIGAVPYVLVHAFPAQVRFSGLSFSYNVSYAIFGGLTPVI
VSLMLKNDSLAPAHYVVGVCIMGIVTALFVRKRHA

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=30.2107728337237, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=28.7410926365796, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=30.1401869158879, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789941, Length=408, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082231, Length=467, Percent_Identity=23.7687366167024, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=363, Percent_Identity=23.4159779614325, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46070; Mature: 45939

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSSSTAGASGAQGGHAARPLTGQDYKTLALAALGGALEFYDFIIFVFFAKVIGQLFFP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASVPDWLRMLQTFGIFAAGYLARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
PTYASVGLLAPMALLVLRILQGAAVGGEVPGAWVFVSEHVPRRHVGYACGTLTAGLTAGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
LLGSLVATGINAVFDPQELVDHGWRVPFVLGGVFGVGSMYLRRWLHETPVFAELQQRKAL
HHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAELPLKTVVRDHRGAVVISMLLTWMLSAGIVVVILLTPTYLQTLYGFDARTALVANSAA
HHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEHHCHHHHHHHHCCCHHHHHHHHHHH
TLCLSIGCVVAGILADRIGARLTLSIGGALLAATACVLYTTIGTRPDLLLPLYALAGFFV
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GTIGAVPYVLVHAFPAQVRFSGLSFSYNVSYAIFGGLTPVIVSLMLKNDSLAPAHYVVGV
HHHHHHHHHHHHHHHHHHEECCCEEEEECHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
CIMGIVTALFVRKRHA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA