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The map label for this gene is ydjQ [H]

Identifier: 94312850

GI number: 94312850

Start: 471045

End: 472637

Strand: Reverse

Name: ydjQ [H]

Synonym: Rmet_3922

Alternate gene names: 94312850

Gene position: 472637-471045 (Counterclockwise)

Preceding gene: 291481498

Following gene: 94312849

Centisome position: 18.32

GC content: 64.85

Gene sequence:

>1593_bases
ATGACCCTCCAAGAACCGCCCACCTACCTTCCAGAGAAGTTGACCAGCCGCGAGCCCGGCATGCTCGATGCACAGGCGCT
TGCTGCCGCGCTTGCCCGGCCTATTGTATTTGTCGATCTGGAAACAACCGGGGCGGACGCGCAGCGAGACCGCATCACCG
AAATCGGTGTGGTCGAAGTCGGCCCCGACGGCATTCAGGAATGGGACACGCTGGTCGATCCCCTGACCAGCATCCCACCG
TTCGTACAGGGACTGACCGGCATCACCGACGAAATGGTCCGCGGCCAGCCCACCTTTGCATTGATTGCCGAAGCCCTGGC
CGAGAAGCTGCAAGGCCGCCTGTTCGTCGCCCACAATGCGCGCTTCGACTATGGGTTTCTGAAGAACGAGTTCCGCCGCG
CTGGCGTGACCTTCCGTGCGGACGTACTTTGCACGCTTCGTCTGTCCCGGTCGCTCTTTCCGTCCGTCGAGCGCCATGGC
CTGGATGCCCTGATCGCCCGCTTCGGCCTGACGCCGAAGGGTCGGCACCGCGCGCTAGCCGATGCCGAGCTGCTCTGGCA
GTTCTGGCAGAAGATTCACGAGATCTATTCGGTCGACCTGGTCGAATCCGCCGTAACGTCGCTGGTCAAGCACGCCAGCC
TGCCAGCGGGTCTGGAAGAAGCTGCATTGGACGATGTACCGGATCGCCCGGGCGTCTATCTCTTCTATGGCGACGACGAC
GTGCCGCTCTACGTTGGCAAGAGCATCCATCTGCGCCAGCGCATTCGCGCCCACTTCGCCGGGGATCACAGCAACGCCAG
GGAGATGCGGCTGTCCCGCTCCGTGCGTCGCGTCGAGTGGCGCGAGACCGGAGGAGAAATCGGCGCACTGCTGAGCGAGG
CGCATCTGGTCAAGACACTGCGCCCGCTGCACAACCACCTGCTGCGCCAGAACACCGAGCTGTTCTCCTGGGAAATGCTC
GACGGGCTGCCTGCGCCGCGCCTGCGTTCGGATCGTCACGTCGATTTCAGTCGACACGCCGCACTATATGGTGCATTTCC
GAGCCGGGCCTCCGCGCAAGCGCGCCTGCGATCGCTGGCGGATGAACACCGGCTTTGCCTGGCGACGCTGATGCTCGAAG
GCACCACCCGCCGGGGCAGCCCTTGTTTCGCGCGGCAGTTGCACCGATGCGCGGGCGCCTGCACCGGGGAGGAATCCCAG
ACGGCCCATCGGGAACGCACCGTGGAAGCAATGGCTTCCATGGCCATCCAGCGCTGGCCGTTTGCAGGAGCAATTGCCTG
GCGTGAAGCTTCCGGCGATCGTTTCCCCTGGCACATCATCGAAGACTGGTGCTACCTGGGCTCGGCCCCCACGCTTGAGG
ACGCCGCCCGGCAATTGGTCGCGCCGGCCCGCTTCGACATCGATACCTACCAGATCCTGGCCCCGCGGATCGAGGAGTTG
AAGGCCAGCGCCGTACCCCTGTTATCTACACGCGAGTTCGTGCTGACGCCGCCGGTCCTCCCGGAGCCCAGAGCGGCCCG
CGTGCCTCGCACGGCGGGAAAGCGTACGGCAAACGCCTCGGAGCACAGTCAGACGCCGGGGCTCTTCAGCTAA

Upstream 100 bases:

>100_bases
GCAGTGCGTTTCGTGTTGCGAGGGGGCGAATATTAGATGACCACACCGCGGAACGCAAGAGGTTTCGTAAAAGAATTTAC
CTATCTATTAGAATCGCCGC

Downstream 100 bases:

>100_bases
AACAGCCGAACGGGCTGGCGAACGGTGCGGCAGCACGCATAAATGCACTGGCAGCCGGCGCCTGGCTCGCCTAGTCTCCG
AGGGGAGGACTGCCGCCCCT

Product: DNA polymerase III, epsilon subunit

Products: diphosphate; DNAn+1

Alternate protein names: Endonuclease cho; UvrC homolog protein [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTLQEPPTYLPEKLTSREPGMLDAQALAAALARPIVFVDLETTGADAQRDRITEIGVVEVGPDGIQEWDTLVDPLTSIPP
FVQGLTGITDEMVRGQPTFALIAEALAEKLQGRLFVAHNARFDYGFLKNEFRRAGVTFRADVLCTLRLSRSLFPSVERHG
LDALIARFGLTPKGRHRALADAELLWQFWQKIHEIYSVDLVESAVTSLVKHASLPAGLEEAALDDVPDRPGVYLFYGDDD
VPLYVGKSIHLRQRIRAHFAGDHSNAREMRLSRSVRRVEWRETGGEIGALLSEAHLVKTLRPLHNHLLRQNTELFSWEML
DGLPAPRLRSDRHVDFSRHAALYGAFPSRASAQARLRSLADEHRLCLATLMLEGTTRRGSPCFARQLHRCAGACTGEESQ
TAHRERTVEAMASMAIQRWPFAGAIAWREASGDRFPWHIIEDWCYLGSAPTLEDAARQLVAPARFDIDTYQILAPRIEEL
KASAVPLLSTREFVLTPPVLPEPRAARVPRTAGKRTANASEHSQTPGLFS

Sequences:

>Translated_530_residues
MTLQEPPTYLPEKLTSREPGMLDAQALAAALARPIVFVDLETTGADAQRDRITEIGVVEVGPDGIQEWDTLVDPLTSIPP
FVQGLTGITDEMVRGQPTFALIAEALAEKLQGRLFVAHNARFDYGFLKNEFRRAGVTFRADVLCTLRLSRSLFPSVERHG
LDALIARFGLTPKGRHRALADAELLWQFWQKIHEIYSVDLVESAVTSLVKHASLPAGLEEAALDDVPDRPGVYLFYGDDD
VPLYVGKSIHLRQRIRAHFAGDHSNAREMRLSRSVRRVEWRETGGEIGALLSEAHLVKTLRPLHNHLLRQNTELFSWEML
DGLPAPRLRSDRHVDFSRHAALYGAFPSRASAQARLRSLADEHRLCLATLMLEGTTRRGSPCFARQLHRCAGACTGEESQ
TAHRERTVEAMASMAIQRWPFAGAIAWREASGDRFPWHIIEDWCYLGSAPTLEDAARQLVAPARFDIDTYQILAPRIEEL
KASAVPLLSTREFVLTPPVLPEPRAARVPRTAGKRTANASEHSQTPGLFS
>Mature_529_residues
TLQEPPTYLPEKLTSREPGMLDAQALAAALARPIVFVDLETTGADAQRDRITEIGVVEVGPDGIQEWDTLVDPLTSIPPF
VQGLTGITDEMVRGQPTFALIAEALAEKLQGRLFVAHNARFDYGFLKNEFRRAGVTFRADVLCTLRLSRSLFPSVERHGL
DALIARFGLTPKGRHRALADAELLWQFWQKIHEIYSVDLVESAVTSLVKHASLPAGLEEAALDDVPDRPGVYLFYGDDDV
PLYVGKSIHLRQRIRAHFAGDHSNAREMRLSRSVRRVEWRETGGEIGALLSEAHLVKTLRPLHNHLLRQNTELFSWEMLD
GLPAPRLRSDRHVDFSRHAALYGAFPSRASAQARLRSLADEHRLCLATLMLEGTTRRGSPCFARQLHRCAGACTGEESQT
AHRERTVEAMASMAIQRWPFAGAIAWREASGDRFPWHIIEDWCYLGSAPTLEDAARQLVAPARFDIDTYQILAPRIEELK
ASAVPLLSTREFVLTPPVLPEPRAARVPRTAGKRTANASEHSQTPGLFS

Specific function: Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5' site of a lesion. When a lesion remains because uvrC is not able to induce the 3' incision, cho

COG id: COG0322

COG function: function code L; Nuclease subunit of the excinuclease complex

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrC C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788037, Length=257, Percent_Identity=40.8560311284047, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786409, Length=177, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081999, Length=178, Percent_Identity=27.5280898876405, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000305 [H]

Pfam domain/function: PF01541 GIY-YIG [H]

EC number: 2.7.7.7

Molecular weight: Translated: 59082; Mature: 58951

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50164 UVRC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEPPTYLPEKLTSREPGMLDAQALAAALARPIVFVDLETTGADAQRDRITEIGVVEV
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCEEEE
GPDGIQEWDTLVDPLTSIPPFVQGLTGITDEMVRGQPTFALIAEALAEKLQGRLFVAHNA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECC
RFDYGFLKNEFRRAGVTFRADVLCTLRLSRSLFPSVERHGLDALIARFGLTPKGRHRALA
CCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHH
DAELLWQFWQKIHEIYSVDLVESAVTSLVKHASLPAGLEEAALDDVPDRPGVYLFYGDDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEECCCC
VPLYVGKSIHLRQRIRAHFAGDHSNAREMRLSRSVRRVEWRETGGEIGALLSEAHLVKTL
CCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RPLHNHLLRQNTELFSWEMLDGLPAPRLRSDRHVDFSRHAALYGAFPSRASAQARLRSLA
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHH
DEHRLCLATLMLEGTTRRGSPCFARQLHRCAGACTGEESQTAHRERTVEAMASMAIQRWP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FAGAIAWREASGDRFPWHIIEDWCYLGSAPTLEDAARQLVAPARFDIDTYQILAPRIEEL
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KASAVPLLSTREFVLTPPVLPEPRAARVPRTAGKRTANASEHSQTPGLFS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLQEPPTYLPEKLTSREPGMLDAQALAAALARPIVFVDLETTGADAQRDRITEIGVVEV
CCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCEEEE
GPDGIQEWDTLVDPLTSIPPFVQGLTGITDEMVRGQPTFALIAEALAEKLQGRLFVAHNA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECC
RFDYGFLKNEFRRAGVTFRADVLCTLRLSRSLFPSVERHGLDALIARFGLTPKGRHRALA
CCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHH
DAELLWQFWQKIHEIYSVDLVESAVTSLVKHASLPAGLEEAALDDVPDRPGVYLFYGDDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEECCCC
VPLYVGKSIHLRQRIRAHFAGDHSNAREMRLSRSVRRVEWRETGGEIGALLSEAHLVKTL
CCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RPLHNHLLRQNTELFSWEMLDGLPAPRLRSDRHVDFSRHAALYGAFPSRASAQARLRSLA
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHH
DEHRLCLATLMLEGTTRRGSPCFARQLHRCAGACTGEESQTAHRERTVEAMASMAIQRWP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FAGAIAWREASGDRFPWHIIEDWCYLGSAPTLEDAARQLVAPARFDIDTYQILAPRIEEL
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KASAVPLLSTREFVLTPPVLPEPRAARVPRTAGKRTANASEHSQTPGLFS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]