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The map label for this gene is hyuA [H]

Identifier: 94312814

GI number: 94312814

Start: 429988

End: 431406

Strand: Reverse

Name: hyuA [H]

Synonym: Rmet_3886

Alternate gene names: 94312814

Gene position: 431406-429988 (Counterclockwise)

Preceding gene: 94312815

Following gene: 94312813

Centisome position: 16.72

GC content: 66.88

Gene sequence:

>1419_bases
ATGCCCGCAGACAACAATCTCGACCTGGTAATCCGCAACGCCGACGTGGTCACCGCCTCCGACCGCTTCCGTTGCGACAT
TGGCGTGCGCGGCGGCCGCATCGTGGCACTTGGCCACGACCTGCCGCCGGCTGCCAGCGAAATCGACGCCACCGGCCTGC
TGGCCTTGCCCGGCGGCGTGGACGGCCACTGCCATCTGGACCAGCCGATGCCCGACGGCATGCGCATGGCCGATGACTTC
TTCACCGGCACGCGCGCGGCTGCGTGCGGCGGCACGACCACCGTGATCCCGTTCGCCGCGCAGGAGAAAGGCGCGTCGCT
CAAGGCAGCCGTGGACGACTATCACCGCCGCGCCGATGGCCGCGCCGTGATCGACTACGCGTTCCACCTGATCGTGGCGG
ACCCCACCCCCGCCGTGCTGCAGGACGAACTGCCGTCACTGATTCGCCAGGGCTATAGCTCCTTCAAGGTCTACATGACC
TATGACGACCTCAAGCTGTCGGACCGCGAGATGCTGTCCGTGCTCGACGTGGCGCGGCAGAACAATGCGCTGGTCATGGT
GCACGCCGAGAATGCCGACTGCATCTCGTGGCTGACCGACAAGCTCGTGGGGCAAGGCCGCATCGCGCCGCGCTTCCACG
CGCTGGCGCGCCCCGATGCCGTCGAACGCGAGGCCACGCATCGCGCCATCACGTTCGCGGAACTGGTGGACGTGCCGATC
CTGATCGTGCACGTCTCCGGCAAGGAAGCCATCGAGCAAATCCGCTGGGCGCAGGGTCGTGGCATGAAGATACTGGCGGA
GACCTGCCCGCAGTACCTGTACCTGACCGCCGAGGACATGGGCATGGGCGGCGACGACGGCTACGAAGGCGCGAAGTGCG
TTTGCAGCCCGCCGCCACGCGACCCGGAGAACCAGGCCGCCGTCTGGCGTGCGCTGACCAATGGCGTATTCAGCGTATTC
TCGTCGGACCATGCGCCGTTCAACTACGACGATCCGGAAGGCAAGAAGCTGGGCGGCACGGCGCAGCCGTTCGACCACAT
CCCCAATGGCGTGCCGGGCATCGAAACCCGCCTGCCGCTGCTGTTCGACGGCGTGGCGCGCGGCAAGCTCTCGCTGCACC
AGTTCGTGGAACTCACGTCGTACCGCCCCGCCAAGATCTACGGACTCTACCCGCGCAAGGGCACGATTGCTGTCGGCGCC
GATGCGGACATCGTGCTCTGGGACCCCACGCGCAAGGTGCGCATCGCCAACGCCAACCTGCACCACGCGGTGGACTACAC
GCCGTATGAAGGCATCGAGGTCACCGGCTGGCCGATGACCACCTTCTCGCGCGGCGAACTGATCGTGCGCAATGGCGAGT
ACCTGGAGCCTGCGGCCGGCCGCGGCAAGTTCCTCGAAGCTGGACAACCCGAGATCTGA

Upstream 100 bases:

>100_bases
AAGTCGCTGCGTCTGGCCGGTGTCGATACCCGCGCGCTGCAGGGTTGGGGCCGCCTGTTCCGCGAAGTCGAATGACCGAC
AATCCATCGGAGTGACGACC

Downstream 100 bases:

>100_bases
TTCGCCATGAACCTGCTCATCGTCAATCCGAATATCAGTGAGTCGGTCACCGACCTGATTCACGCCGAGGCCCAGCGCAC
TGTGTCGCCGGACACCCGCA

Product: phenylhydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPADNNLDLVIRNADVVTASDRFRCDIGVRGGRIVALGHDLPPAASEIDATGLLALPGGVDGHCHLDQPMPDGMRMADDF
FTGTRAAACGGTTTVIPFAAQEKGASLKAAVDDYHRRADGRAVIDYAFHLIVADPTPAVLQDELPSLIRQGYSSFKVYMT
YDDLKLSDREMLSVLDVARQNNALVMVHAENADCISWLTDKLVGQGRIAPRFHALARPDAVEREATHRAITFAELVDVPI
LIVHVSGKEAIEQIRWAQGRGMKILAETCPQYLYLTAEDMGMGGDDGYEGAKCVCSPPPRDPENQAAVWRALTNGVFSVF
SSDHAPFNYDDPEGKKLGGTAQPFDHIPNGVPGIETRLPLLFDGVARGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGA
DADIVLWDPTRKVRIANANLHHAVDYTPYEGIEVTGWPMTTFSRGELIVRNGEYLEPAAGRGKFLEAGQPEI

Sequences:

>Translated_472_residues
MPADNNLDLVIRNADVVTASDRFRCDIGVRGGRIVALGHDLPPAASEIDATGLLALPGGVDGHCHLDQPMPDGMRMADDF
FTGTRAAACGGTTTVIPFAAQEKGASLKAAVDDYHRRADGRAVIDYAFHLIVADPTPAVLQDELPSLIRQGYSSFKVYMT
YDDLKLSDREMLSVLDVARQNNALVMVHAENADCISWLTDKLVGQGRIAPRFHALARPDAVEREATHRAITFAELVDVPI
LIVHVSGKEAIEQIRWAQGRGMKILAETCPQYLYLTAEDMGMGGDDGYEGAKCVCSPPPRDPENQAAVWRALTNGVFSVF
SSDHAPFNYDDPEGKKLGGTAQPFDHIPNGVPGIETRLPLLFDGVARGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGA
DADIVLWDPTRKVRIANANLHHAVDYTPYEGIEVTGWPMTTFSRGELIVRNGEYLEPAAGRGKFLEAGQPEI
>Mature_471_residues
PADNNLDLVIRNADVVTASDRFRCDIGVRGGRIVALGHDLPPAASEIDATGLLALPGGVDGHCHLDQPMPDGMRMADDFF
TGTRAAACGGTTTVIPFAAQEKGASLKAAVDDYHRRADGRAVIDYAFHLIVADPTPAVLQDELPSLIRQGYSSFKVYMTY
DDLKLSDREMLSVLDVARQNNALVMVHAENADCISWLTDKLVGQGRIAPRFHALARPDAVEREATHRAITFAELVDVPIL
IVHVSGKEAIEQIRWAQGRGMKILAETCPQYLYLTAEDMGMGGDDGYEGAKCVCSPPPRDPENQAAVWRALTNGVFSVFS
SDHAPFNYDDPEGKKLGGTAQPFDHIPNGVPGIETRLPLLFDGVARGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGAD
ADIVLWDPTRKVRIANANLHHAVDYTPYEGIEVTGWPMTTFSRGELIVRNGEYLEPAAGRGKFLEAGQPEI

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=467, Percent_Identity=38.3297644539615, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI4503051, Length=463, Percent_Identity=34.7732181425486, Blast_Score=260, Evalue=3e-69,
Organism=Homo sapiens, GI62422571, Length=463, Percent_Identity=34.7732181425486, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI4503377, Length=464, Percent_Identity=35.1293103448276, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI190194363, Length=461, Percent_Identity=35.5748373101952, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI4503379, Length=463, Percent_Identity=34.1252699784017, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI19923821, Length=465, Percent_Identity=34.8387096774194, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI18105007, Length=484, Percent_Identity=24.5867768595041, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI87082175, Length=464, Percent_Identity=38.7931034482759, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1786722, Length=472, Percent_Identity=30.9322033898305, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17539558, Length=465, Percent_Identity=34.8387096774194, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71989490, Length=465, Percent_Identity=33.9784946236559, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI86575075, Length=458, Percent_Identity=27.0742358078603, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=415, Percent_Identity=24.3373493975904, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322218, Length=408, Percent_Identity=29.9019607843137, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI221377917, Length=477, Percent_Identity=35.8490566037736, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17137462, Length=465, Percent_Identity=34.6236559139785, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24644287, Length=308, Percent_Identity=35.0649350649351, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24644289, Length=232, Percent_Identity=36.6379310344828, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24642586, Length=427, Percent_Identity=27.6346604215457, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859883, Length=428, Percent_Identity=28.2710280373832, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- InterPro:   IPR011612 [H]

Pfam domain/function: PF01979 Amidohydro_1; PF00449 Urease_alpha [H]

EC number: 3.5.2.2

Molecular weight: Translated: 51372; Mature: 51241

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADNNLDLVIRNADVVTASDRFRCDIGVRGGRIVALGHDLPPAASEIDATGLLALPGGV
CCCCCCEEEEEECCEEEEECCCEEEEEECCCCEEEEECCCCCCCCHHCCCCEEEECCCCC
DGHCHLDQPMPDGMRMADDFFTGTRAAACGGTTTVIPFAAQEKGASLKAAVDDYHRRADG
CCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCC
RAVIDYAFHLIVADPTPAVLQDELPSLIRQGYSSFKVYMTYDDLKLSDREMLSVLDVARQ
CEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC
NNALVMVHAENADCISWLTDKLVGQGRIAPRFHALARPDAVEREATHRAITFAELVDVPI
CCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
LIVHVSGKEAIEQIRWAQGRGMKILAETCPQYLYLTAEDMGMGGDDGYEGAKCVCSPPPR
EEEEECCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEHHCCCCCCCCCCCCEEEECCCCC
DPENQAAVWRALTNGVFSVFSSDHAPFNYDDPEGKKLGGTAQPFDHIPNGVPGIETRLPL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
LFDGVARGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGADADIVLWDPTRKVRIANANL
EECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEECCCCEEEEECCCC
HHAVDYTPYEGIEVTGWPMTTFSRGELIVRNGEYLEPAAGRGKFLEAGQPEI
EEEECCCCCCCEEEECCCCCEECCCEEEEECCCEECCCCCCCCEECCCCCCC
>Mature Secondary Structure 
PADNNLDLVIRNADVVTASDRFRCDIGVRGGRIVALGHDLPPAASEIDATGLLALPGGV
CCCCCEEEEEECCEEEEECCCEEEEEECCCCEEEEECCCCCCCCHHCCCCEEEECCCCC
DGHCHLDQPMPDGMRMADDFFTGTRAAACGGTTTVIPFAAQEKGASLKAAVDDYHRRADG
CCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCC
RAVIDYAFHLIVADPTPAVLQDELPSLIRQGYSSFKVYMTYDDLKLSDREMLSVLDVARQ
CEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC
NNALVMVHAENADCISWLTDKLVGQGRIAPRFHALARPDAVEREATHRAITFAELVDVPI
CCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCE
LIVHVSGKEAIEQIRWAQGRGMKILAETCPQYLYLTAEDMGMGGDDGYEGAKCVCSPPPR
EEEEECCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEHHCCCCCCCCCCCCEEEECCCCC
DPENQAAVWRALTNGVFSVFSSDHAPFNYDDPEGKKLGGTAQPFDHIPNGVPGIETRLPL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
LFDGVARGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGADADIVLWDPTRKVRIANANL
EECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEECCCCEEEEECCCC
HHAVDYTPYEGIEVTGWPMTTFSRGELIVRNGEYLEPAAGRGKFLEAGQPEI
EEEECCCCCCCEEEECCCCCEECCCEEEEECCCEECCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA