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The map label for this gene is nac [H]

Identifier: 94312763

GI number: 94312763

Start: 374530

End: 375468

Strand: Reverse

Name: nac [H]

Synonym: Rmet_3833

Alternate gene names: 94312763

Gene position: 375468-374530 (Counterclockwise)

Preceding gene: 94312770

Following gene: 94312762

Centisome position: 14.55

GC content: 67.84

Gene sequence:

>939_bases
ATGGACATCCGTGCCATGCGCTATTTTGTGGCCATCGTCGACCAAGGCAGCCTGACCCGCGCCGCCGAGGTCGTCTGCGT
GGCGCAGCCCGCGCTGTCGCAGCAGCTTGCCACGCTGGAAGACGAATTCGGCGTGCCATTGGTGCACCGCAGCGCGAAAG
GCGTGCGGCCCACCGAAGCCGGAAAGACGCTTTACCGTCACGTGCGCAACATCCTTCGACAGGTGGAAATCGCGGGCGCC
GACGTGCGCGTGGCCGGAGCCGAGGTCTCGGGCACCGTGGCCATCGGCCTGCCCACCACGGCGGCAGCGGCCTTCGGCAT
GGCGCTGATACGCACCGTGCGCCAGCGCTACCCGCAGGTCCGGCTGCAGCTCTTTGAAAGCATGAGCGGATATATCTCCG
AACTGCTGAACCAGAACCGGCTCGACTTCGCCATCCTGTTTCGCGACACGCCATCGCGCGCGGTGGAGCCGGAGCCGCTG
GCCAGCGAACCGCTCTACCTGATCGGCACGCCACCCGTGACCGCGCCGCGCCCGGGCAAACGGGGCAGCGCCACGGCGGA
TACGGTCCCGCTCAAGGCGCTCGACGGCATCCCGCTGGTACTGCCAAGCGGCTCGCAGGGGTTGCGCGAGGTGGTCGAGC
GCACGTTTGCCAAGGCCGGCATGAATCTGAATGTGGTGGCCGATCTCGACTCCCTGCCTTTCCTGCTGGCGTCCGCCCGG
GAAGGATTGGCCTGCACGATACTGCCAGCGTCGTCGCTTGGCGATGGCGATGCGACCGTGCCTCGGCGGCTGATCGTTCC
GGAACTCCGACGCACGCTGTCACTGTGCTGGCCGCGCGCGCTGCCGCGCTCGAATGCGGCCGAGGCGGTGGCTGATGTGT
TGCGAGAGATAGTCGGAGAACGGATTGCGCTGGGCGGGTCGCTGGCGCCAGCGGGTTGA

Upstream 100 bases:

>100_bases
GCCCGCCTCCGACCTGTGAAATAGGGATTGCAGAAGGCTCCATAAGTAGAATCAATAGCCTGCACCCCCGGTGCGTCCCC
ATGCACCCTCTGGAGAACCC

Downstream 100 bases:

>100_bases
TGGGAGCGGCGACTCCCGGTGTCGGTGTATGGATGGCCGGCCCGCCGCGCCCGGTCCGATAAGAAAACCTTATGCCCATA
GTCACCGATTGAATTTCTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDIRAMRYFVAIVDQGSLTRAAEVVCVAQPALSQQLATLEDEFGVPLVHRSAKGVRPTEAGKTLYRHVRNILRQVEIAGA
DVRVAGAEVSGTVAIGLPTTAAAAFGMALIRTVRQRYPQVRLQLFESMSGYISELLNQNRLDFAILFRDTPSRAVEPEPL
ASEPLYLIGTPPVTAPRPGKRGSATADTVPLKALDGIPLVLPSGSQGLREVVERTFAKAGMNLNVVADLDSLPFLLASAR
EGLACTILPASSLGDGDATVPRRLIVPELRRTLSLCWPRALPRSNAAEAVADVLREIVGERIALGGSLAPAG

Sequences:

>Translated_312_residues
MDIRAMRYFVAIVDQGSLTRAAEVVCVAQPALSQQLATLEDEFGVPLVHRSAKGVRPTEAGKTLYRHVRNILRQVEIAGA
DVRVAGAEVSGTVAIGLPTTAAAAFGMALIRTVRQRYPQVRLQLFESMSGYISELLNQNRLDFAILFRDTPSRAVEPEPL
ASEPLYLIGTPPVTAPRPGKRGSATADTVPLKALDGIPLVLPSGSQGLREVVERTFAKAGMNLNVVADLDSLPFLLASAR
EGLACTILPASSLGDGDATVPRRLIVPELRRTLSLCWPRALPRSNAAEAVADVLREIVGERIALGGSLAPAG
>Mature_312_residues
MDIRAMRYFVAIVDQGSLTRAAEVVCVAQPALSQQLATLEDEFGVPLVHRSAKGVRPTEAGKTLYRHVRNILRQVEIAGA
DVRVAGAEVSGTVAIGLPTTAAAAFGMALIRTVRQRYPQVRLQLFESMSGYISELLNQNRLDFAILFRDTPSRAVEPEPL
ASEPLYLIGTPPVTAPRPGKRGSATADTVPLKALDGIPLVLPSGSQGLREVVERTFAKAGMNLNVVADLDSLPFLLASAR
EGLACTILPASSLGDGDATVPRRLIVPELRRTLSLCWPRALPRSNAAEAVADVLREIVGERIALGGSLAPAG

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=38.7351778656126, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=31.5972222222222, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=28.8590604026846, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790399, Length=300, Percent_Identity=29.6666666666667, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=31.7241379310345, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693105, Length=278, Percent_Identity=23.3812949640288, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789506, Length=302, Percent_Identity=24.5033112582781, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788887, Length=251, Percent_Identity=27.8884462151394, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=26.8211920529801, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=172, Percent_Identity=30.8139534883721, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788626, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=259, Percent_Identity=25.8687258687259, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367136, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=24.0802675585284, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=25.531914893617, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082132, Length=193, Percent_Identity=25.9067357512953, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33228; Mature: 33228

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRAMRYFVAIVDQGSLTRAAEVVCVAQPALSQQLATLEDEFGVPLVHRSAKGVRPTEA
CCHHHHEEEHHHCCCCCCHHHHHEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHH
GKTLYRHVRNILRQVEIAGADVRVAGAEVSGTVAIGLPTTAAAAFGMALIRTVRQRYPQV
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
RLQLFESMSGYISELLNQNRLDFAILFRDTPSRAVEPEPLASEPLYLIGTPPVTAPRPGK
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
RGSATADTVPLKALDGIPLVLPSGSQGLREVVERTFAKAGMNLNVVADLDSLPFLLASAR
CCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCC
EGLACTILPASSLGDGDATVPRRLIVPELRRTLSLCWPRALPRSNAAEAVADVLREIVGE
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RIALGGSLAPAG
HHHCCCCCCCCC
>Mature Secondary Structure
MDIRAMRYFVAIVDQGSLTRAAEVVCVAQPALSQQLATLEDEFGVPLVHRSAKGVRPTEA
CCHHHHEEEHHHCCCCCCHHHHHEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHH
GKTLYRHVRNILRQVEIAGADVRVAGAEVSGTVAIGLPTTAAAAFGMALIRTVRQRYPQV
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
RLQLFESMSGYISELLNQNRLDFAILFRDTPSRAVEPEPLASEPLYLIGTPPVTAPRPGK
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
RGSATADTVPLKALDGIPLVLPSGSQGLREVVERTFAKAGMNLNVVADLDSLPFLLASAR
CCCCCCCCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCC
EGLACTILPASSLGDGDATVPRRLIVPELRRTLSLCWPRALPRSNAAEAVADVLREIVGE
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
RIALGGSLAPAG
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]