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The map label for this gene is clcD [H]

Identifier: 94312577

GI number: 94312577

Start: 178632

End: 179342

Strand: Reverse

Name: clcD [H]

Synonym: Rmet_3645

Alternate gene names: 94312577

Gene position: 179342-178632 (Counterclockwise)

Preceding gene: 94312578

Following gene: 94312574

Centisome position: 6.95

GC content: 65.96

Gene sequence:

>711_bases
ATGACCACCAATAGCCGCTGGATTCGTATCGATACCCCCGATGGCAGCTTCGACGCCTATCTGAGCCTGCCGCCAGCAGG
CAAGCAGCCGGGCAACCCGGGCATCGTGCTGATCCAGGAGATCTTCGGCGTCAACGAACACATCCGCTCCGTGGCGGACC
AGTACGCGGCCGACGGCTACGTGGTGCTGGCGCCGGACGTGTTCTGGCGCACGGCGCCGCGCGTGGAACTTGGCTACTCG
GGCAAGGACTGGGAAGAGGCCATGAAGCTGCGCCAGGCCGTGAACCTGGAGGAGGCCGTATCCGACATCGGTGCGACGGC
CAAGGCGCTGCGCGCGGAAATCGGCGCCAGCGGTAAGGTGGCGGCAGTGGGTTACTGTTTTGGCGGGCTGCTGTCGTACA
TGGCGGCCGCGCGTGGCATGGTCGATGCGGCGGTGCCGTACTACGGCGGCGGTATCCAGAACAACCTCAAGGAAGCAGAA
GCGCTCAACGTCCCGGTGCAGTTCCACTATGGCGCGCTCGACGCCCACATCAAGCCCGAGGACGTCGAACGTGTGCGTCA
GGCTGTGGCGGGCAAGCGCGGTGTCGAGGTCTTCGTCTATGACCAGGCCGACCACGGCTTCAACTGCTGGGCGCGTGGCT
CGTACCACCAGCGTTCGGCGGCGCTCGCGCATGGCCGCGCGCTGACGTTCCTGACTAACGCGCTGTCCTGA

Upstream 100 bases:

>100_bases
ACCCATGTAGCGCGTGGTCCGCTGCACGATTACCTTACCGGTGGCAAACTTGCGCCCTGGTTCCTCACCCTCTGAACACT
TCACCCCGGAGTTTCCGACC

Downstream 100 bases:

>100_bases
CGCTTCAATGCAGCCGCCGTTCCGCTGCGCAGTACGTCTTCGACGGTTTCAACTGCTGCAGCGGGCCCGGCGCGCTCAAT
ACGGCATCGCCACACCGTTC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGYVVLAPDVFWRTAPRVELGYS
GKDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKVAAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAE
ALNVPVQFHYGALDAHIKPEDVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS

Sequences:

>Translated_236_residues
MTTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGYVVLAPDVFWRTAPRVELGYS
GKDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKVAAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAE
ALNVPVQFHYGALDAHIKPEDVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS
>Mature_235_residues
TTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGYVVLAPDVFWRTAPRVELGYSG
KDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKVAAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAEA
LNVPVQFHYGALDAHIKPEDVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=210, Percent_Identity=30.4761904761905, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 25487; Mature: 25356

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGY
CCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHCCCCE
VVLAPDVFWRTAPRVELGYSGKDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKV
EEECCHHHHCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
AAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAEALNVPVQFHYGALDAHIKPE
EHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCEECCCCHH
DVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGY
CCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHCCCCE
VVLAPDVFWRTAPRVELGYSGKDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKV
EEECCHHHHCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
AAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAEALNVPVQFHYGALDAHIKPE
EHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEECCCEECCCCHH
DVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3299368 [H]