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The map label for this gene is ascD [H]

Identifier: 94312562

GI number: 94312562

Start: 166189

End: 167193

Strand: Reverse

Name: ascD [H]

Synonym: Rmet_3630

Alternate gene names: 94312562

Gene position: 167193-166189 (Counterclockwise)

Preceding gene: 94312563

Following gene: 94312561

Centisome position: 6.48

GC content: 65.97

Gene sequence:

>1005_bases
ATGTCCTATCGGGTCGAGATTGCGGAAACGCAGCAGGTTTTCATGGTGGCGCCCGGCGAGTCCGTGCTCGATGCCGCCCT
GCGCTGCGGCGTCAACCTGGCGCACGAATGCACCTTCGGCGGCTGTGGCACGTGCCGCGTGCGCGTGGTCGACGGTGCCG
TGACCTATGAAGAATTCCCGATGGGACTGACGGAGGAAGAGGACGCAGCCGGCTTCGCGCTGGCCTGCCAGGCCCGGCCC
GCCGGCGACCTCGTCATCAGCACCGCGCGCGCGGCGGAACCCGCGATTCCGGCGCGGCGTACCACCGCGACGGTTCAGGC
CATCACCTCGCTCGGACCCGATGTGCTGCATCTGCGGCTCGCGTTGTCCGACGACGAGGCGTTCGCGTTCGAACCGGGCC
AGTACATGAAGATCCTGCTCGAGGACGGCAGCCATCGCAGCTTCTCGATGGCATCGGCCCCGAACGGGAACACGGTGGAC
TTTCACGTACGGCAGATCGCGGGCGGACGCTTCACCACGGGCCAGCTTCCGCAACTACGGCCGGGCGACAAGCTCGACGT
CGAAGTCCCGCACGGCAGCTTCAGCCTGCGCAAGGAAGACTACCGGCCGCTGCTGATGGTGGCCACCGGCACCGGGCTCG
CGCCGATCAAGAGCATGCTCGAGGCACTGATGGATGATCCCGACTGTCCTCCCGTATGGCTCTACTGGGGGATGCGCTCG
GCAGCGGATCTCTATCTCCATGACGAGATTGCCCGGTGGGGCGAGCGGCTCTACGATTTCCAGTACGTGCCGGTGCTGTC
ACGCGCGGACGATACCTGGCAGGGTCGGCGCGGCTACGTGCACGACGCGGTGGCGGCCGATATCGGCGACTTGTCCGAAC
ACGCGATCTATCTGTGCGGCTCTCCGAACATGATCCACGACGCCAAACAGACATTCATGGTGCTGGGCGCGCAGGCGCCA
TTCATCTATTCCGACGGATTCACGTTCCAGCACGCGCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGTGGCCGCGCTCGAAGCGTTGCGCGACCTGCTGGCGGCGGAAGACCTGACCGTGCTGGCCCAGGCCGATATCAAGAAG
TAATCAGGGAAGGAGTCGTC

Downstream 100 bases:

>100_bases
CGCCGGCGCGAGAACGCGCATCTCAATGTCTACCCTATGTCTGCACTCAGGGGGTGACGGTATACAATAGCGGCATTCAC
GCCGTGGCCCAGCGCAGCGG

Product: multidomain oxidoreductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFPMGLTEEEDAAGFALACQARP
AGDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRLALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVD
FHVRQIAGGRFTTGQLPQLRPGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRS
AADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCGSPNMIHDAKQTFMVLGAQAP
FIYSDGFTFQHAPG

Sequences:

>Translated_334_residues
MSYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFPMGLTEEEDAAGFALACQARP
AGDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRLALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVD
FHVRQIAGGRFTTGQLPQLRPGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRS
AADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCGSPNMIHDAKQTFMVLGAQAP
FIYSDGFTFQHAPG
>Mature_333_residues
SYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFPMGLTEEEDAAGFALACQARPA
GDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRLALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVDF
HVRQIAGGRFTTGQLPQLRPGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRSA
ADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCGSPNMIHDAKQTFMVLGAQAPF
IYSDGFTFQHAPG

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=231, Percent_Identity=30.7359307359307, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 36449; Mature: 36317

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFP
CCEEEEEECCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECEEEHHHCC
MGLTEEEDAAGFALACQARPAGDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRL
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
ALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVDFHVRQIAGGRFTTGQLPQLR
EECCCCEEEECCCCEEEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCC
PGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRS
CCCEEEEEECCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
AADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCG
CCCEEEHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEC
SPNMIHDAKQTFMVLGAQAPFIYSDGFTFQHAPG
CCCHHHHHHHEEEEEECCCCEEEECCEEEECCCC
>Mature Secondary Structure 
SYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFP
CEEEEEECCCEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECEEEHHHCC
MGLTEEEDAAGFALACQARPAGDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRL
CCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
ALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVDFHVRQIAGGRFTTGQLPQLR
EECCCCEEEECCCCEEEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCC
PGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRS
CCCEEEEEECCCCCEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
AADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCG
CCCEEEHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEC
SPNMIHDAKQTFMVLGAQAPFIYSDGFTFQHAPG
CCCHHHHHHHEEEEEECCCCEEEECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]