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The map label for this gene is nifA1 [H]

Identifier: 291481565

GI number: 291481565

Start: 2067930

End: 2069255

Strand: Reverse

Name: nifA1 [H]

Synonym: NA

Alternate gene names: 291481565

Gene position: 2069255-2067930 (Counterclockwise)

Preceding gene: 94314275

Following gene: 94314267

Centisome position: 80.2

GC content: 66.67

Gene sequence:

>1326_bases
ATGGATCGCCCCACCACACTCCAGGGACTGTTGTCCTACCTGGAACAGGAAAGCCAGCCGACGATCGTGCTGGATACCGA
CTACCGCATCCTCGCTGCCAATACGGCCTACCGCCGCCAGTTTGGCGCGGTGGAACGCCCCTATCTGGGCCAGCCCTGCT
ACCGCGTCTCGCACGGCTATGATGTGCCGTGCGATCAGGCCGGCGAACACTGTCCGATGCGCAAGGCGTTCGAGACCCGT
GGGCCGGATCGCGTCCTTCACATCCATCACACCCCGCGTGGCCCCGAGCATGTGAACGTGGAACTGCGCCCGATCCTCGA
CGACAACCACCAGGTGGTGGCTTACGTCGAGCGCCTGAACACCGTGCGAAGCGCATCGGCGCGGCCGAGCGCGGAAGGGC
TGGTGGGACGCTCGGAGGCCTTCAATCACGCGCTGGAGGCCGTGCAGCGCGTAGCACCGTCGTCGTTACCGGTGCTGCTG
CTCGGTGAATCGGGCACTGGCAAGGAGATGTTCGCGCGCGCCGTGCACGAGGCCAGCGACCGCGCCAGCGGGCCGTTCGT
CGTGGTCGATTGCTCGGGCCTGACGGAGACCCTGTTCGAGAGTGAGCTGTTCGGCTACGAGAAGGGCGCGTTCACCGGCG
CGCAGGCGCGTAAGCCGGGGCTGGTCGAGACGGCCGAGGGCGGCACGCTATTCCTCGACGAAATGGGCGATGTGCCGCTG
GCCATGCAGGTCAAGCTGCTGCGCCTGATCGAGTCGGGTACTTACCGGCGCGTGGGTGGCGTCGAGACGCTGCAGTCGAA
CTTCCGGTTGGTTGCCGCCACGCACAAGCCGCTCGATCAGATGGTGGCGCAGGGGCTGTTTCGCCAGGATCTGTACTTCC
GGATCGCGGCATTCCCGATCCATCTGCCGTCGCTGCGAGACCGCCTGGACGATATCGCGCTGCTGGCCGAGTCGATTCTG
GCGCGCGGCAATCGGACCAAGGACCCGCTGCGGCTGAGTTCTGAGGCGCTCGCGCGCATGCGCGCTCACCACTGGCCGGG
CAATATTCGCGAACTGCGCAACGTGCTGGAGCGCGCCCGTCTCTATGCCGACGACGGTGTGATCCGCGTGAAGGATCTGC
CGCCGGGAATGGGCGAGGCCCGTGTGTCTTCGGTCCGCTTCGTGCCGCCGTCCGCGTATCGCACGCAGGCGCCACGGCAG
GATGACGATGAGGCGCTGCGTTTCCACGCAAGCCACTTCACGGGCACGCGCCGCGCGCTTGCCCAATCGCTCGGCATGAG
CGAACGCACCCTCTATCGCCGGCTCAAGGCGCTGGGCATCGAATAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: transcriptional regulatory protein, sigma-54 interaction domain, HTH Fis-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:
NA

Sequences:

>Translated_441_residues
MDRPTTLQGLLSYLEQESQPTIVLDTDYRILAANTAYRRQFGAVERPYLGQPCYRVSHGYDVPCDQAGEHCPMRKAFETR
GPDRVLHIHHTPRGPEHVNVELRPILDDNHQVVAYVERLNTVRSASARPSAEGLVGRSEAFNHALEAVQRVAPSSLPVLL
LGESGTGKEMFARAVHEASDRASGPFVVVDCSGLTETLFESELFGYEKGAFTGAQARKPGLVETAEGGTLFLDEMGDVPL
AMQVKLLRLIESGTYRRVGGVETLQSNFRLVAATHKPLDQMVAQGLFRQDLYFRIAAFPIHLPSLRDRLDDIALLAESIL
ARGNRTKDPLRLSSEALARMRAHHWPGNIRELRNVLERARLYADDGVIRVKDLPPGMGEARVSSVRFVPPSAYRTQAPRQ
DDDEALRFHASHFTGTRRALAQSLGMSERTLYRRLKALGIE
>Mature_441_residues
MDRPTTLQGLLSYLEQESQPTIVLDTDYRILAANTAYRRQFGAVERPYLGQPCYRVSHGYDVPCDQAGEHCPMRKAFETR
GPDRVLHIHHTPRGPEHVNVELRPILDDNHQVVAYVERLNTVRSASARPSAEGLVGRSEAFNHALEAVQRVAPSSLPVLL
LGESGTGKEMFARAVHEASDRASGPFVVVDCSGLTETLFESELFGYEKGAFTGAQARKPGLVETAEGGTLFLDEMGDVPL
AMQVKLLRLIESGTYRRVGGVETLQSNFRLVAATHKPLDQMVAQGLFRQDLYFRIAAFPIHLPSLRDRLDDIALLAESIL
ARGNRTKDPLRLSSEALARMRAHHWPGNIRELRNVLERARLYADDGVIRVKDLPPGMGEARVSSVRFVPPSAYRTQAPRQ
DDDEALRFHASHFTGTRRALAQSLGMSERTLYRRLKALGIE

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=313, Percent_Identity=39.9361022364217, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789087, Length=344, Percent_Identity=39.5348837209302, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=41.1764705882353, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=39.1566265060241, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=40.9937888198758, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=48.9177489177489, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=43.9330543933054, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1790299, Length=364, Percent_Identity=36.5384615384615, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1786524, Length=320, Percent_Identity=39.0625, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=43.5344827586207, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787583, Length=381, Percent_Identity=32.0209973753281, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789828, Length=288, Percent_Identity=36.8055555555556, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=32.2147651006711, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49121; Mature: 49121

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPTTLQGLLSYLEQESQPTIVLDTDYRILAANTAYRRQFGAVERPYLGQPCYRVSHGY
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHCCCCCCCCCCCHHCCCCCC
DVPCDQAGEHCPMRKAFETRGPDRVLHIHHTPRGPEHVNVELRPILDDNHQVVAYVERLN
CCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
TVRSASARPSAEGLVGRSEAFNHALEAVQRVAPSSLPVLLLGESGTGKEMFARAVHEASD
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
RASGPFVVVDCSGLTETLFESELFGYEKGAFTGAQARKPGLVETAEGGTLFLDEMGDVPL
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCH
AMQVKLLRLIESGTYRRVGGVETLQSNFRLVAATHKPLDQMVAQGLFRQDLYFRIAAFPI
HHHHHHHHHHHCCCCEECCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEE
HLPSLRDRLDDIALLAESILARGNRTKDPLRLSSEALARMRAHHWPGNIRELRNVLERAR
ECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LYADDGVIRVKDLPPGMGEARVSSVRFVPPSAYRTQAPRQDDDEALRFHASHFTGTRRAL
HCCCCCEEEEECCCCCCCCHHHCEEEECCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHH
AQSLGMSERTLYRRLKALGIE
HHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRPTTLQGLLSYLEQESQPTIVLDTDYRILAANTAYRRQFGAVERPYLGQPCYRVSHGY
CCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHCCCCCCCCCCCHHCCCCCC
DVPCDQAGEHCPMRKAFETRGPDRVLHIHHTPRGPEHVNVELRPILDDNHQVVAYVERLN
CCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
TVRSASARPSAEGLVGRSEAFNHALEAVQRVAPSSLPVLLLGESGTGKEMFARAVHEASD
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
RASGPFVVVDCSGLTETLFESELFGYEKGAFTGAQARKPGLVETAEGGTLFLDEMGDVPL
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCH
AMQVKLLRLIESGTYRRVGGVETLQSNFRLVAATHKPLDQMVAQGLFRQDLYFRIAAFPI
HHHHHHHHHHHCCCCEECCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEE
HLPSLRDRLDDIALLAESILARGNRTKDPLRLSSEALARMRAHHWPGNIRELRNVLERAR
ECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LYADDGVIRVKDLPPGMGEARVSSVRFVPPSAYRTQAPRQDDDEALRFHASHFTGTRRAL
HCCCCCEEEEECCCCCCCCHHHCEEEECCCHHHHCCCCCCCCHHHHHHHHHHCCHHHHHH
AQSLGMSERTLYRRLKALGIE
HHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2836706; 2710108 [H]