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The map label for this gene is mcbR [H]

Identifier: 291481513

GI number: 291481513

Start: 667831

End: 668514

Strand: Reverse

Name: mcbR [H]

Synonym: NA

Alternate gene names: 291481513

Gene position: 668514-667831 (Counterclockwise)

Preceding gene: 94313031

Following gene: 94313024

Centisome position: 25.91

GC content: 68.13

Gene sequence:

>684_bases
ATGTCGGAAGAAAAGAAGAGCCGCACCGAGACCATCAGGTCCGAGATAGAGCAGGAAATCCTGTCCGGGCACCTGCCGAG
CGGCACCCAGATCGATGAACAGCAACTGATCGACCGTTTCGGCGTGTCCCGCACGCCCGCGCGCGAGGCGCTGATCCAGC
TCGCCTCGGCTGGACTCGTCACCCTGGTGCCGCGCCAGGGCGCGATCGTGTCGTCGATTACATTGCGCGACTACGTCGCG
CTGAGTGAGATCCTGGTCCAGCTGGAATCGCTTGCCGCCCGCCTCGCCTCGCGCCGCATGACGCCGGCGGAGCGTGACGC
GCTGGCGACCGCGTACGAAGCGTGCGCGGCCGCGCAGGGAGATGCCGAGGCCTACCGCGCCGCCAACGACGAATTCCATG
GCGTGCTCTACGAGGGCAGCCGCAACGACATCCTGGCCAAGCAGATCCGCACCATGCGCGCGCGGATGCGTGGCATGCGG
GACTACCGCTTCGAGCACCCCGCGCGCGTTCGCGCATCGCTGGCCGAGCACAAGGCCGTGCTCGACGCGATCAAGGTTGG
CGACGAGGATGGCGCCGCCGAAGCGATGGTCCGGCACATCTCCGCGGGCGGCAATGTCTATGCCGACATGATCGCGGCGA
TGCCCGACGATGGCGCGGCGGTCGGCCGCGGACGTCGCCCGTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: transcriptional regulator (GntR-family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:
NA

Sequences:

>Translated_227_residues
MSEEKKSRTETIRSEIEQEILSGHLPSGTQIDEQQLIDRFGVSRTPAREALIQLASAGLVTLVPRQGAIVSSITLRDYVA
LSEILVQLESLAARLASRRMTPAERDALATAYEACAAAQGDAEAYRAANDEFHGVLYEGSRNDILAKQIRTMRARMRGMR
DYRFEHPARVRASLAEHKAVLDAIKVGDEDGAAEAMVRHISAGGNVYADMIAAMPDDGAAVGRGRRP
>Mature_226_residues
SEEKKSRTETIRSEIEQEILSGHLPSGTQIDEQQLIDRFGVSRTPAREALIQLASAGLVTLVPRQGAIVSSITLRDYVAL
SEILVQLESLAARLASRRMTPAERDALATAYEACAAAQGDAEAYRAANDEFHGVLYEGSRNDILAKQIRTMRARMRGMRD
YRFEHPARVRASLAEHKAVLDAIKVGDEDGAAEAMVRHISAGGNVYADMIAAMPDDGAAVGRGRRP

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=203, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787821, Length=184, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24731; Mature: 24600

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKKSRTETIRSEIEQEILSGHLPSGTQIDEQQLIDRFGVSRTPAREALIQLASAGLV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCE
TLVPRQGAIVSSITLRDYVALSEILVQLESLAARLASRRMTPAERDALATAYEACAAAQG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
DAEAYRAANDEFHGVLYEGSRNDILAKQIRTMRARMRGMRDYRFEHPARVRASLAEHKAV
CHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LDAIKVGDEDGAAEAMVRHISAGGNVYADMIAAMPDDGAAVGRGRRP
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEEKKSRTETIRSEIEQEILSGHLPSGTQIDEQQLIDRFGVSRTPAREALIQLASAGLV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCE
TLVPRQGAIVSSITLRDYVALSEILVQLESLAARLASRRMTPAERDALATAYEACAAAQG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
DAEAYRAANDEFHGVLYEGSRNDILAKQIRTMRARMRGMRDYRFEHPARVRASLAEHKAV
CHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LDAIKVGDEDGAAEAMVRHISAGGNVYADMIAAMPDDGAAVGRGRRP
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]