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The map label for this gene is tam [H]

Identifier: 94312543

GI number: 94312543

Start: 3923729

End: 3924499

Strand: Reverse

Name: tam [H]

Synonym: Rmet_3612

Alternate gene names: 94312543

Gene position: 3924499-3923729 (Counterclockwise)

Preceding gene: 94312544

Following gene: 94312542

Centisome position: 99.91

GC content: 67.19

Gene sequence:

>771_bases
ATGACCTGGTCGGCCAGCCAATACGTGCAATTCGAGGATGAACGGACCCGCCCCGTGCGGGATCTGGTCGGGGCGATCCC
CACCGCCAGCGCGCGGCGGGCCGCCGATGTCGGCTGCGGCCCGGGCAACTCCACCGAAGTACTGGCCGCGCGCTACGCCG
GCGCCGAGGTAACGGGGTTCGACAGCGACGCGGACATGATTGCGGCGGCACGCAAACGCCTGCCGGACCTGCGCTTCGAT
ATCGCCGACGTCACCGCCTGGAATGACCCCGGCCCCTACGACGTGATCCTGGCCAATGCCGTGTTCCAGTGGGTGCCAGA
TCACGCCACGCTGTTTCCCTCGCTGATCGACAAGCTAGCGCCGGGCGGCAGCCTGGCCGTGCAGATGCCGGACAACCTCA
ACGAGCCGCCGCACCAGTTGATGCGCGAGACAGCGCTGAACGGCCCGTGGGCGAACAAGCTCGCCAAGGCCGCCGAGGCC
CGCACGCCGCTGGCCAACGAGCGCTGGTACTACGAACTGCTCAAACCGCACTGCACACGCGTGGACATCTGGCGCACGAT
CTACCAACATCCGCTCAAGGGCGGCCCGGCGGCCATCGTCGAGTGGTTCAAGGGCAGCGGCTTGCGGCCGTTCCTGGCGC
CTCTGACCGAAGATGAGAAGGCCGAATACCTGAAGCGATATGAGGCGGCAGTGGCCAAGGTCTTTCCGGCCATGTCCGAT
GGCACGGTCCTGCTGCCGTTCCCGCGCGTGTTCATCGTCGCAACGCGCTAG

Upstream 100 bases:

>100_bases
CCGGCCTCCTGCCGGGTTTTTTCTTTTTCGGCGCACGTCACTAGTCTTATATCTTATATAAGACTAGAATCCCCTTCACC
AAGACGAAAGGGGATACGCC

Downstream 100 bases:

>100_bases
GAAGCCTCCACGGAACATGGCGTTGGTATCACGTGCGTCGTCGCCTACACTGCAGTGACGCCTTGCAGTCTCCTCGTGTC
CAAGCTGTGCGCAGAGAGAT

Product: trans-aconitate methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGFDSDADMIAAARKRLPDLRFD
IADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLAPGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEA
RTPLANERWYYELLKPHCTRVDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSD
GTVLLPFPRVFIVATR

Sequences:

>Translated_256_residues
MTWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGFDSDADMIAAARKRLPDLRFD
IADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLAPGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEA
RTPLANERWYYELLKPHCTRVDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSD
GTVLLPFPRVFIVATR
>Mature_255_residues
TWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGFDSDADMIAAARKRLPDLRFDI
ADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLAPGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEAR
TPLANERWYYELLKPHCTRVDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSDG
TVLLPFPRVFIVATR

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=254, Percent_Identity=45.2755905511811, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 28275; Mature: 28144

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGF
CCCCCCCCEEECCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEECC
DSDADMIAAARKRLPDLRFDIADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLA
CCCHHHHHHHHHHCCCCEEEHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
PGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEARTPLANERWYYELLKPHCTR
CCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCH
VDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSD
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTVLLPFPRVFIVATR
CEEEEECCEEEEEECC
>Mature Secondary Structure 
TWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGF
CCCCCCCEEECCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEECC
DSDADMIAAARKRLPDLRFDIADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLA
CCCHHHHHHHHHHCCCCEEEHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
PGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEARTPLANERWYYELLKPHCTR
CCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCH
VDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSD
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTVLLPFPRVFIVATR
CEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]