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The map label for this gene is trmE

Identifier: 94312541

GI number: 94312541

Start: 3921381

End: 3922808

Strand: Reverse

Name: trmE

Synonym: Rmet_3610

Alternate gene names: 94312541

Gene position: 3922808-3921381 (Counterclockwise)

Preceding gene: 94312542

Following gene: 94312540

Centisome position: 99.87

GC content: 64.64

Gene sequence:

>1428_bases
ATGACCGTATCCCAGATTCCCATCGCCGCGATTGCCACCGCGCCTGGCCGTGGCGGCATCGGCGTCGTGCGTGTTTCCGG
CCCCGATGTCGGCGCCGTGATGCGCGCCGTATGCGGCCGCGCGCTGCAGCCACGTCACGCCACCTATCTGCCGTTTCTCG
ATGCGCGTGGCAATGTCATCGATCATGGCCTCGCGCTCTACTTCCCGGCACCGAATTCGTACACCGGCGAGGAAGTGCTT
GAGTTGCAGGGCCATGGCGGACCGGTGGTGATGCAGATGCTGCTGTCGCGCTGCCTCGAGGCCGGCAAGGACATCGGCCT
GCGTGTGGCCGAGCCGGGCGAGTTCACACGACGCGCGTTCCTCAACGACAAGCTCGACCTCGCGCAGGCCGAGGCCGTGG
CGGATCTGATCGAAGCAAGCACGGAGGCCGCGGCGCGGTCTGCGGCGCGGTCCATGGAGGGAGAGTTCTCCAAGGCAATC
CACGCGCTTGTGGAAAAAGTCATCCACCTGCGAATGCTGGTGGAAGCCACGCTGGACTTTCCCGAAGAGGAGATCGATTT
CCTCGAGGCCTCCAATGCACGCGGCCAGCTGACACGCATTCGCGAAGATCTCGCGGGCGTCCTCAAGCAGGCGCGGCAGG
GCTCATTGCTGCGTGAAGGATTGTCGGTGGTTCTGGCCGGACAGCCCAATGTGGGCAAGTCCTCGCTGCTCAATGCGCTC
GCCGGCTCGGATCTCGCCATCGTCACGCCAATTGCCGGCACCACGCGCGATCGCGTGCGCGAGACGATCCAGATAGACGG
CATCCCGCTGCACATTATCGACACCGCAGGTCTGCGCGACGATGCCGCCGACGAAGTGGAACGCATCGGCATCGAGCGCA
CATGGGAAGCCATCCGCCACGCCGATATCGTGCTGCATCTGATCGACGCGGCGGACTACATCGAGCATGGCATTTCGGAG
ACCGATGACCATATCGACGATCGCCTGAGCGGCCAGTTGCCCCCCGGGTCTCCAATCGTTCGCGTCATCAACAAGATCGA
CCTCGCGCCATCCGTCGGCGCGATGGGATTTGGCGGCAACCGCCCGCATGTGGTGGCGGCCAATGGGCCAAACCCGACCG
AGATCTGGATCTCCGCACGCACCGGCGCGGGCATCGACCTGATGCGCAGCGAACTGCTGCGCCTGATCGGCTGGCAGTCC
GGCAATGAAGGCGCGTTCCTGGCGCGGGAGCGCCATCTCATCGCGCTGCGCAACGCGGAATCGCACCTCGAACTCGCCGA
GGCCAGCGCCTCGCAGCACGCCCAGGCGCTGGATCTATTCGCGGAGGAACTGCGGCTGGCACAGGATCACCTGAACAGCA
TCACTGGAGAGTTCACGAGCGATGACCTGCTGGGGACAATTTTCACGCGGTTCTGCATCGGGAAGTGA

Upstream 100 bases:

>100_bases
GACGACGCCGTGGCGCACAGGCTGATATGCGAATGCCTGGCAAGCTGAGCCTTGCCACCCCGATCGTCCCCCATGCGGAC
GATCTGCGACAATACTGGCC

Downstream 100 bases:

>100_bases
CGGCTTGGCGAGACAAGACCGGTAGCACAAGGCGAACCGTCTCCTGATGTCTTCCGCGAAAACCGCTCGCCCCCTCACCC
CCGGTGAGATCGCGCTCGCC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVIDHGLALYFPAPNSYTGEEVL
ELQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAI
HALVEKVIHLRMLVEATLDFPEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNAL
AGSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRHADIVLHLIDAADYIEHGISE
TDDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGNRPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQS
GNEGAFLARERHLIALRNAESHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK

Sequences:

>Translated_475_residues
MTVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVIDHGLALYFPAPNSYTGEEVL
ELQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAI
HALVEKVIHLRMLVEATLDFPEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNAL
AGSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRHADIVLHLIDAADYIEHGISE
TDDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGNRPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQS
GNEGAFLARERHLIALRNAESHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK
>Mature_474_residues
TVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVIDHGLALYFPAPNSYTGEEVLE
LQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAIH
ALVEKVIHLRMLVEATLDFPEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNALA
GSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRHADIVLHLIDAADYIEHGISET
DDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGNRPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQSG
NEGAFLARERHLIALRNAESHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=482, Percent_Identity=35.6846473029046, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI193082988, Length=512, Percent_Identity=33.59375, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI193082993, Length=480, Percent_Identity=34.7916666666667, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI2367268, Length=473, Percent_Identity=54.7568710359408, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87082120, Length=129, Percent_Identity=36.4341085271318, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=472, Percent_Identity=29.6610169491525, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=30.3571428571429, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=30.5498981670061, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_RALME (Q1LH94)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585751.1
- ProteinModelPortal:   Q1LH94
- STRING:   Q1LH94
- GeneID:   4040459
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3610
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q1LH94
- ProtClustDB:   PRK05291
- BioCyc:   RMET266264:RMET_3610-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50991; Mature: 50859

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 124-124 BINDING 475-475

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVI
CCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEE
DHGLALYFPAPNSYTGEEVLELQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAF
CCCEEEEECCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
LNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAIHALVEKVIHLRMLVEATLDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNAL
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCHHHHHHHH
AGSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRH
CCCCEEEEECCCCCCHHHHHHHEEECCEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHH
ADIVLHLIDAADYIEHGISETDDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
RPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQSGNEGAFLARERHLIALRNAE
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCH
SHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVI
CCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEE
DHGLALYFPAPNSYTGEEVLELQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAF
CCCEEEEECCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
LNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAIHALVEKVIHLRMLVEATLDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNAL
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCCCHHHHHHHH
AGSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRH
CCCCEEEEECCCCCCHHHHHHHEEECCEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHH
ADIVLHLIDAADYIEHGISETDDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
RPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQSGNEGAFLARERHLIALRNAE
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCH
SHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA