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The map label for this gene is livM5 [H]

Identifier: 94312447

GI number: 94312447

Start: 3807085

End: 3808119

Strand: Reverse

Name: livM5 [H]

Synonym: Rmet_3516

Alternate gene names: 94312447

Gene position: 3808119-3807085 (Counterclockwise)

Preceding gene: 94312448

Following gene: 94312446

Centisome position: 96.95

GC content: 65.02

Gene sequence:

>1035_bases
ATGAGTGGACCGACCGGACAAACGACTGCGGCCGTGGAGGCCAAGGGCTTCAAGGAAGGAACGGGCGTGCAGAAGAAAGT
GTTGTATGGCCTGCTGTTGCTGGGCCTGATCGCGGCGCCGCTGGCCGGCGCCTACCCGGTGTTCGTGCTCAAGGTGCTGT
GCTTCGCGCTGTTCGCGTGCGCCTTCAACCTGCTGATCGGCTTCACGGGCCTGCTGTCGTTCGGCCACGCGGCGTTCTTC
GGCGGCGCGGGCTATGCGGCAGGGCACGCGATGAAGGTCTGGGGCGTGACACCCGAGCTCGGCCTGATCTTCGGCACGCT
GAGCGGCGCGGCGATCGGCTACGTGGTGGGCTCGCTGGCGATTCGCCGCCAGGGCATCTACTTCTCGATGATCACGCTGG
CGCTGGCGCAGATGCTGTTCTTCTTCTGCCTGCAGGTGCCGTTGACGGGTGGTGAGGACGGGCTGCAGGGCATTCCGCGC
GGCAAGCTGCTTGGCGTGCTGCCGCTTTCCGACGACCTGACCCTCTACTACGTGGCCCTGGTCATCATCGTGGCGGCGTT
CGCGCTGATCGTGCGCACGGTGCACTCGCCGTTCGGCCAAATCTTGAAGGCCATCCGCGAGAACGAGCCGCGTGCCATTT
CTCTGGGTTACGATGTCGACCGCTTCAAGCTGCTGGCCTTCGTGCTTTCGGCAGCGCTCTCCGGTCTGGCGGGCTCGATC
AAGGCGCTGGTGCTCGGCTTCGAGACGCTGACGGACGTGCACTGGTCGATGTCGGGCTCGGTGATCCTGATGACGCTGGT
GGGCGGACTGGGCACACTGTCCGGCCCGATCGTCGGTGCGTTCGTGGTGGTGGCGCTCGAGAACAAGCTGGGCGATATCG
GCACGTTCCTGGCCTCGGTGACCGGCCTCGACTGGTTCAATTCGCTGGGCGAATCGGTGACGATGGTGACGGGCGTGATC
TTCGTGATCTGCGTGCTGACGTTCCGCCGCGGGATCATGGGCGAGCTGATCGCACTCACGAGCCGGAAGAAATAG

Upstream 100 bases:

>100_bases
CCAAGGTGTTCTACCCTGAAGCATCGTCGACTGTGGTGTTCTTCATCATGGTGATCGTGCTGATGCTGCGCCCGGCAGGG
CTGTTCGGGAGGGAAAAGTG

Downstream 100 bases:

>100_bases
CAAGGGTGTGAAGACGGGTCGACATGCCCGATTTTGGGGCTAGGGTTATTGCTGACTTGTTTCATACGCACCGCTTCGTT
ACATTCCATATACGGTTTTC

Product: ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFACAFNLLIGFTGLLSFGHAAFF
GGAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPR
GKLLGVLPLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSI
KALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASVTGLDWFNSLGESVTMVTGVI
FVICVLTFRRGIMGELIALTSRKK

Sequences:

>Translated_344_residues
MSGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFACAFNLLIGFTGLLSFGHAAFF
GGAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPR
GKLLGVLPLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSI
KALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASVTGLDWFNSLGESVTMVTGVI
FVICVLTFRRGIMGELIALTSRKK
>Mature_343_residues
SGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFACAFNLLIGFTGLLSFGHAAFFG
GAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPRG
KLLGVLPLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSIK
ALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASVTGLDWFNSLGESVTMVTGVIF
VICVLTFRRGIMGELIALTSRKK

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=272, Percent_Identity=26.1029411764706, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36022; Mature: 35891

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFAC
CCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFNLLIGFTGLLSFGHAAFFGGAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLA
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPRGKLLGVLPLSDDLTLYYVAL
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
VIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLDWFNSLGESVTMVTGVIFVICVLTFRRGIMGELIALTSRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFAC
CCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFNLLIGFTGLLSFGHAAFFGGAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLA
HHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPRGKLLGVLPLSDDLTLYYVAL
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
VIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
KALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLDWFNSLGESVTMVTGVIFVICVLTFRRGIMGELIALTSRKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]