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The map label for this gene is gidB

Identifier: 94312436

GI number: 94312436

Start: 3794179

End: 3794886

Strand: Reverse

Name: gidB

Synonym: Rmet_3505

Alternate gene names: 94312436

Gene position: 3794886-3794179 (Counterclockwise)

Preceding gene: 94312437

Following gene: 94312435

Centisome position: 96.61

GC content: 63.56

Gene sequence:

>708_bases
GTGGGTGGTTCCCGACATTTCGCGGTCGACGACGCCGCGCAACGCCGCCGGCTCGAAGCCGGACTGGACGCGATCGGGCT
GGCACTGACACCAGCCCAGGTCGATACGCTTTTCGCGTACCTGACGCTGCTCCGCAAATGGAACGGCGTCTACAACCTCA
CCGCGATTCGTCATCCCGACGAAATGCTCACGCATCATCTGCTCGATTCGCTGACGGCTGTTCCGGCGTTGGCCGAAGCC
GCTCGCTCCGCAAACGTGGCGCAAGGCGCGCGGGGCAGGGTGCTCGACGTCGGCTCGGGCGGTGGGATGCCGGGCATGCC
GCTGGCGATTTCGTGCCCGGATGTGTCGGTGCTGATGGTCGACATCGTGCAGAAAAAGACCGCATTCCTGACGCAGTGCC
GTGCCCAGCTGCACCTGACCAATGCGGCTGCCCACTGGGGGCCTGTGGAAAAGATCGACGACGAGCAGGGCTATGCCGTG
ATCACCTCGCGCGCATTCGCAGAATTGACCGACTTCGTGACGTTGTCCGGCCACCTGCTGGCGCCGGGCGGAAAGCTGAT
CGCCATGAAGGGAGTTTATCCACAGGCGGAGATCGATCGAATGGAAGCGGCCGGTCTGATGGCTGACTGGCAGGTCGAAG
CGGTACCGAAACTGGTAGTACCTGAGCTCGATGTGGAACGGCACCTTGTGGTGCTGTCCCGGCGTTGA

Upstream 100 bases:

>100_bases
CTCGCTGCTGTTGGTACACCTGAAGAAGAAGGGCATGGGGCGCGCCGCGGCTCAGACATCGGCAACCCCGGTGGCCGGCA
ATGGACAGGAGGCTGCCTGA

Downstream 100 bases:

>100_bases
AATCGGTTGGGTAATTTATTGAGCACGTGTTCCGCATGGCGGAATTGTGCACTGAGGAGCTGCAAGCGCATATGGCCAAG
GTATTCGTGATCGCAAACCA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPDEMLTHHLLDSLTAVPALAEA
ARSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAV
ITSRAFAELTDFVTLSGHLLAPGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR

Sequences:

>Translated_235_residues
MGGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPDEMLTHHLLDSLTAVPALAEA
ARSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAV
ITSRAFAELTDFVTLSGHLLAPGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR
>Mature_234_residues
GGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPDEMLTHHLLDSLTAVPALAEAA
RSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAVI
TSRAFAELTDFVTLSGHLLAPGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=217, Percent_Identity=40.5529953917051, Blast_Score=151, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_RALME (Q1LHJ9)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585646.1
- ProteinModelPortal:   Q1LHJ9
- SMR:   Q1LHJ9
- STRING:   Q1LHJ9
- GeneID:   4040353
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3505
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   Q1LHJ9
- ProtClustDB:   PRK00107
- BioCyc:   RMET266264:RMET_3505-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 25291; Mature: 25159

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPD
CCCCCCEEECHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
EMLTHHLLDSLTAVPALAEAARSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHH
DIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAVITSRAFAELTDFVTLSGHLL
HHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHEECCEEE
APGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHCCCHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure 
GGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPD
CCCCCEEECHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
EMLTHHLLDSLTAVPALAEAARSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHH
DIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAVITSRAFAELTDFVTLSGHLL
HHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHEECCEEE
APGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR
CCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHCCCHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA