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The map label for this gene is ybdK [C]

Identifier: 94312416

GI number: 94312416

Start: 3768616

End: 3769734

Strand: Reverse

Name: ybdK [C]

Synonym: Rmet_3485

Alternate gene names: 94312416

Gene position: 3769734-3768616 (Counterclockwise)

Preceding gene: 94312417

Following gene: 94312409

Centisome position: 95.97

GC content: 62.65

Gene sequence:

>1119_bases
ATGTCGCTCGAACCCTTCAAGCATTCCGAAGCCCTGACGTTTGGTGTCGAACTGGAACTGCAGCTCGTCAATCGTCATGA
CTATGACCTCGCGCCGTTCGCGCCGGACCTGCTCCGGGCGCTCAGGGGCACGCAACATGCCGGCGACATCAAGCCGGAGA
TCAGTCCGTCGATGATCGAGATCAGCACGGGGATCTGCCACGACTACGCGCAGGCGCTCGAGGAGCTGAGCGTCATGCGG
GACCTGATGGTCAATGCGTCACGCGGCTTGAACCTCGGCATCTGCGGCGGCGGCACGCACCCGTTCCAGGTGTGGTCCGA
TCGCACGATTTCCGACTCGCCCCGCTACCAGTACATCTCCGAACTCTACGGCTATCTGGCCAAGCAGTTCACGGTGTTTG
GGCAGCACGTCCATATCGGGTGTCCGAGCCCCGACGAGTCGCTGTTCCTGCTGCACGCCATCGGGCGCTACGTCCCGCAC
TTCATTGCGCTGGCCGCGTCGTCGCCCTATGTGCAGGGTGTGGATACCGGTTTCGCTTCGGCCCGTCTGAATTCGGTGGC
GGCATTCCCGATGAGCGGGCGCGCGCCGTTCGTGCTGACTTGGGACGCCTTCAAGGCGTACTTCGACAAGATGCGCGCCA
CCGGTGTGATCGAGAGCATGAAGGACTTCTATTGGGACATTCGTCCCAAGCCGGAATTCGGCACGATCGAAGTTCGCGTC
ATGGACACGCCACTGACCGTGCAGCGCGCGTGCGACATCGCGGCCTATATCCAGGCGCTGGCGCGTTACCTGCTGTTGTC
ACGGCCATTCATGCCGCAGGAAGACGACTATCTGGTCTACACATTCAACCGCTTCCAGGCGTGTCGCTTCGGGCTGGAGG
GCGAGTATGTGCATCCGAATGAACTGACTCGGATGCCGATTGCCGACCATATCCTGTCGATCTGCGATGCGCTCGAGCCA
CATGCGGAAGCACTGGGCTCGCTGGAGGCGCTATCGAATATCCGCGCGCTGGCGGCGAGCCGCAGCAACGACGCGCATTG
GGTGCGCGCCGCCAATCGCGATGCGCACAGCCTGCGCGATATGGTGCGACAGACCTGCGAACAGTGGGGGGGCGGCTGA

Upstream 100 bases:

>100_bases
TCATTCTGTGTGCGCTGGCGATCGAGCAGTTGCTCGGACCCGTCATCGCGGCGTGGGCGCTGCATTATGTCAGCGAAGCG
CGTGACAACGGAGGGCGCTG

Downstream 100 bases:

>100_bases
GGCAAGGCGCTGCGCCGACGCTGCGCAGCGCTCAACTCACGCGCCGTGCGGCACACTTTCCCCGTCGCGGCCGGTGATCA
GACCGAGTTGCTGGTTGGCC

Product: gamma-glutamyl:cysteine ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIEISTGICHDYAQALEELSVMR
DLMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYISELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPH
FIALAASSPYVQGVDTGFASARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRV
MDTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPNELTRMPIADHILSICDALEP
HAEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRDMVRQTCEQWGGG

Sequences:

>Translated_372_residues
MSLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIEISTGICHDYAQALEELSVMR
DLMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYISELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPH
FIALAASSPYVQGVDTGFASARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRV
MDTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPNELTRMPIADHILSICDALEP
HAEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRDMVRQTCEQWGGG
>Mature_371_residues
SLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIEISTGICHDYAQALEELSVMRD
LMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYISELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPHF
IALAASSPYVQGVDTGFASARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRVM
DTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPNELTRMPIADHILSICDALEPH
AEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRDMVRQTCEQWGGG

Specific function: ATP-dependent carboxylate-amine ligase

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family

Homologues:

Organism=Escherichia coli, GI1786795, Length=371, Percent_Identity=46.6307277628032, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CAAL_RALME (Q1LHL9)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585626.1
- ProteinModelPortal:   Q1LHL9
- SMR:   Q1LHL9
- STRING:   Q1LHL9
- GeneID:   4040333
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3485
- eggNOG:   COG2170
- HOGENOM:   HBG564677
- OMA:   DKPRFQE
- PhylomeDB:   Q1LHL9
- ProtClustDB:   PRK13516
- BioCyc:   RMET266264:RMET_3485-MONOMER
- HAMAP:   MF_01609
- InterPro:   IPR011793
- InterPro:   IPR006336
- TIGRFAMs:   TIGR02050

Pfam domain/function: PF04107 GCS2

EC number: NA

Molecular weight: Translated: 41654; Mature: 41523

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIE
CCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
ISTGICHDYAQALEELSVMRDLMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYIS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCHHHHHHH
ELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPHFIALAASSPYVQGVDTGFAS
HHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRV
HHHHHEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
MDTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPN
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCCEECCC
ELTRMPIADHILSICDALEPHAEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRD
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHH
MVRQTCEQWGGG
HHHHHHHHCCCC
>Mature Secondary Structure 
SLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIE
CCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
ISTGICHDYAQALEELSVMRDLMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYIS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCHHHHHHH
ELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPHFIALAASSPYVQGVDTGFAS
HHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
ARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRV
HHHHHEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
MDTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPN
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHCCCCCCEECCC
ELTRMPIADHILSICDALEPHAEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRD
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHH
MVRQTCEQWGGG
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA