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The map label for this gene is 94312400

Identifier: 94312400

GI number: 94312400

Start: 3748244

End: 3749635

Strand: Reverse

Name: 94312400

Synonym: Rmet_3469

Alternate gene names: NA

Gene position: 3749635-3748244 (Counterclockwise)

Preceding gene: 94312401

Following gene: 94312399

Centisome position: 95.46

GC content: 66.31

Gene sequence:

>1392_bases
ATGCCCAACCTGACCACTGATCATATCGACATGCTGCGCCACGCCGTGCGCGGCAAGGTGCTGCTCCCCGCCGATGCAGG
ATACGAAACAGCACGTACGGTCTGGAATGCCACCGTCGACAGGCGTCCGGCGATCATCGTCCAGTGTTCGGGCACGGCCG
ACGTGATGCAGGCCGTCGGCTTCGCGCGCGACCGTGGCCTGGTGCTCGCGGTGCGGGGCGGCGGGCACAACATCGCTGGC
AGCGCGATCTGCAACGGCGGTCTCGTCATCGACCTGTCGCAACTGCGTACCGTCCACGTGGACCCACTCGAACGCGTAGC
GTGGGTGAGTCCCGGCGCCACGCTCGCCGATTTCGACCATGAGGCGCAGGCGCAGGGTCTGGCCACGCCGCTCGGGATCA
ATTCGACCACCGGCGTAGCTGGACTGACACTTGGTGGAGGCTTCGGCTGGCTCACGCGCAAGTACGGCATGACTGTGGAT
AACCTGCTCGGCTGCGAGATCGTCACCGCTGACGGCACGCGTCACTGGACCGATTCGCGACATGAGCCTGAGCTGTTCTG
GGCGTTGCGCGGCGGTGGCGGCAACTTCGGCGTGGTGACGCTCTTCCAGTTCCGACTGCATCCCGTGGGGCCGATGATCA
CGGCGGGCCTGCTGGTCTTCCCGGCGGTCGAGGCGAAGGCAGTGCTCCGCCAATACCGCGCCTATGTGGAATCGACGATG
CCCGAGGATCTCAACGTCTGGGTCGTGCTGCGTAAAGCGCCGCCGCTGCCCTTCCTGCCGGCCTCCGCGCATGGCAAGGA
TGTGGTGGTGCTGGCGGTGTTCCACGACGGCGATCCGGCCGCCGCGGAGAAGGCGATCGAACCGCTGCGCAAGTTCGGGG
AGACCGTGGGCGAACACGTCGGACAGATGCCTTACACCGCGTGGCAACAGGCCTTCGACGCGTTGCTTGGTCCGGGTGCC
CGCAACTACTGGAAGTCGCACAATTTCACTCGGCTCGAGGATGGGGCGATCGACGCCATGACGGACTTCGCACTGCGGCT
ACCGTCGCCGCTGGCGGACATCTTCGTCGGGCAGGTGGGCGGCGTGGCCAATCGCGTGGCGCCCGATGCCACCGCGTATC
ACCATCGCGACGCCCGCTATGTGCTGAACGTTCACGCGCGGTGGGAGCGTCCCGACGAGGACGCCGCCTGTATCGCCTGG
GCGCGCGACTTTTTCCGCGCCACAGAGACGTTCGCCACTGGCGGCGTCTACGTGAACTTCCTGACCGACGATGAAACCGC
GCGCATCGGCGCGGCCTATGGCCCGAACTACGCCCGCCTGGCGCAGATCAAGCGCACCTACGATCCGCAGAACCTGTTCT
CCACGAATCAGAACATCGCGCCGGCGTCCTGA

Upstream 100 bases:

>100_bases
AGCGCCAATGCGGCTTTCCGCCCATTGGCGCGTGCCTTGCATGGATATCTCCTCCGGGTGCGAATTCCGAAGCCCCGGGC
ATGTTTGTGGAGGTACCGCC

Downstream 100 bases:

>100_bases
TACCGATTGCTCCACATCGTCGGCCACAGCGTTGCTATGATGCGGCGCATGGCCGACGATTTTTTCATCCCACATCAGGA
GTACGACATCACCGCGATCC

Product: FAD/FMN-containing oxidoreductase

Products: NA

Alternate protein names: 6-HDNO [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPNLTTDHIDMLRHAVRGKVLLPADAGYETARTVWNATVDRRPAIIVQCSGTADVMQAVGFARDRGLVLAVRGGGHNIAG
SAICNGGLVIDLSQLRTVHVDPLERVAWVSPGATLADFDHEAQAQGLATPLGINSTTGVAGLTLGGGFGWLTRKYGMTVD
NLLGCEIVTADGTRHWTDSRHEPELFWALRGGGGNFGVVTLFQFRLHPVGPMITAGLLVFPAVEAKAVLRQYRAYVESTM
PEDLNVWVVLRKAPPLPFLPASAHGKDVVVLAVFHDGDPAAAEKAIEPLRKFGETVGEHVGQMPYTAWQQAFDALLGPGA
RNYWKSHNFTRLEDGAIDAMTDFALRLPSPLADIFVGQVGGVANRVAPDATAYHHRDARYVLNVHARWERPDEDAACIAW
ARDFFRATETFATGGVYVNFLTDDETARIGAAYGPNYARLAQIKRTYDPQNLFSTNQNIAPAS

Sequences:

>Translated_463_residues
MPNLTTDHIDMLRHAVRGKVLLPADAGYETARTVWNATVDRRPAIIVQCSGTADVMQAVGFARDRGLVLAVRGGGHNIAG
SAICNGGLVIDLSQLRTVHVDPLERVAWVSPGATLADFDHEAQAQGLATPLGINSTTGVAGLTLGGGFGWLTRKYGMTVD
NLLGCEIVTADGTRHWTDSRHEPELFWALRGGGGNFGVVTLFQFRLHPVGPMITAGLLVFPAVEAKAVLRQYRAYVESTM
PEDLNVWVVLRKAPPLPFLPASAHGKDVVVLAVFHDGDPAAAEKAIEPLRKFGETVGEHVGQMPYTAWQQAFDALLGPGA
RNYWKSHNFTRLEDGAIDAMTDFALRLPSPLADIFVGQVGGVANRVAPDATAYHHRDARYVLNVHARWERPDEDAACIAW
ARDFFRATETFATGGVYVNFLTDDETARIGAAYGPNYARLAQIKRTYDPQNLFSTNQNIAPAS
>Mature_462_residues
PNLTTDHIDMLRHAVRGKVLLPADAGYETARTVWNATVDRRPAIIVQCSGTADVMQAVGFARDRGLVLAVRGGGHNIAGS
AICNGGLVIDLSQLRTVHVDPLERVAWVSPGATLADFDHEAQAQGLATPLGINSTTGVAGLTLGGGFGWLTRKYGMTVDN
LLGCEIVTADGTRHWTDSRHEPELFWALRGGGGNFGVVTLFQFRLHPVGPMITAGLLVFPAVEAKAVLRQYRAYVESTMP
EDLNVWVVLRKAPPLPFLPASAHGKDVVVLAVFHDGDPAAAEKAIEPLRKFGETVGEHVGQMPYTAWQQAFDALLGPGAR
NYWKSHNFTRLEDGAIDAMTDFALRLPSPLADIFVGQVGGVANRVAPDATAYHHRDARYVLNVHARWERPDEDAACIAWA
RDFFRATETFATGGVYVNFLTDDETARIGAAYGPNYARLAQIKRTYDPQNLFSTNQNIAPAS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012951
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR006093 [H]

Pfam domain/function: PF08031 BBE; PF01565 FAD_binding_4 [H]

EC number: =1.5.3.6 [H]

Molecular weight: Translated: 50186; Mature: 50055

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00862 OX2_COVAL_FAD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLTTDHIDMLRHAVRGKVLLPADAGYETARTVWNATVDRRPAIIVQCSGTADVMQAVG
CCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHC
FARDRGLVLAVRGGGHNIAGSAICNGGLVIDLSQLRTVHVDPLERVAWVSPGATLADFDH
CCCCCCEEEEEECCCCCCCCCEEECCCEEEEHHHCEEEECCHHHHHEECCCCCEEECCCC
EAQAQGLATPLGINSTTGVAGLTLGGGFGWLTRKYGMTVDNLLGCEIVTADGTRHWTDSR
HHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCCC
HEPELFWALRGGGGNFGVVTLFQFRLHPVGPMITAGLLVFPAVEAKAVLRQYRAYVESTM
CCCEEEEEEECCCCCEEHEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PEDLNVWVVLRKAPPLPFLPASAHGKDVVVLAVFHDGDPAAAEKAIEPLRKFGETVGEHV
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GQMPYTAWQQAFDALLGPGARNYWKSHNFTRLEDGAIDAMTDFALRLPSPLADIFVGQVG
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHC
GVANRVAPDATAYHHRDARYVLNVHARWERPDEDAACIAWARDFFRATETFATGGVYVNF
CHHHHCCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEE
LTDDETARIGAAYGPNYARLAQIKRTYDPQNLFSTNQNIAPAS
EECCCCCEEECCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
PNLTTDHIDMLRHAVRGKVLLPADAGYETARTVWNATVDRRPAIIVQCSGTADVMQAVG
CCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHC
FARDRGLVLAVRGGGHNIAGSAICNGGLVIDLSQLRTVHVDPLERVAWVSPGATLADFDH
CCCCCCEEEEEECCCCCCCCCEEECCCEEEEHHHCEEEECCHHHHHEECCCCCEEECCCC
EAQAQGLATPLGINSTTGVAGLTLGGGFGWLTRKYGMTVDNLLGCEIVTADGTRHWTDSR
HHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCCC
HEPELFWALRGGGGNFGVVTLFQFRLHPVGPMITAGLLVFPAVEAKAVLRQYRAYVESTM
CCCEEEEEEECCCCCEEHEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PEDLNVWVVLRKAPPLPFLPASAHGKDVVVLAVFHDGDPAAAEKAIEPLRKFGETVGEHV
CCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
GQMPYTAWQQAFDALLGPGARNYWKSHNFTRLEDGAIDAMTDFALRLPSPLADIFVGQVG
CCCCHHHHHHHHHHHHCCCHHHHHCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHC
GVANRVAPDATAYHHRDARYVLNVHARWERPDEDAACIAWARDFFRATETFATGGVYVNF
CHHHHCCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCEEEEE
LTDDETARIGAAYGPNYARLAQIKRTYDPQNLFSTNQNIAPAS
EECCCCCEEECCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3622516; 2680607; 2115879 [H]