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The map label for this gene is kefF [H]

Identifier: 94312308

GI number: 94312308

Start: 3653154

End: 3653813

Strand: Reverse

Name: kefF [H]

Synonym: Rmet_3377

Alternate gene names: 94312308

Gene position: 3653813-3653154 (Counterclockwise)

Preceding gene: 94312310

Following gene: 94312307

Centisome position: 93.02

GC content: 65.15

Gene sequence:

>660_bases
GTGGATTTGCTGCAACGCCGCATTCTCCTGCTGCCGGCCACTCGCTGGACCCGTTCGTACCCAGACACCATGACCGAGCC
CCCCGTAGTTGTGATCTTCGCGCATCCGTCACCCAGGCGTTCACGCGTTAACCGTCCCCTCGCTGAAGCGCTGGCTGCGT
TGCCCCATGTGGAAGTGCGCGAGTTGTACCGGCTCTACGTCGACTACGACATCGATGTCGTCGCCGAGCAACGCCTGCTG
TCCACCGCCGAGACCCTGGTCCTGCAGTTTCCCGTGCGCTGGTACAGCGTGCCCGCGCTTCTCAAGCTGTGGCTGGATGA
TGTGCTCGAAAGCGGCTGGGCCTTTGGCCCCGGCGGCACGGCGCTGCGTGGCAAGTCGATGCTGGCTGTGGTCAGCACCG
GTGGTCATGCCGATGCCTATGGCCCGGAGGGCACTCATGGCCATCCAATCGCCGATTTCCTGCTGCCGCTCGAACAGACC
GCGCTGTTGTGCGGCATGACCTGGCTCCCGCCGGTGGTGCTGCATGACGCCGACAACGCCGATAGCGATGCCGTGGCGCT
CCATATCGAGCATGTGGCGCGTCATCTGCCGGCGCTACCGCTACCCCCTGAAACGCAACAGTGGACCGATGCGGAGCTGG
GCGGGGAGGACGCGCCATGA

Upstream 100 bases:

>100_bases
ATCCTGCGCCGCAACACACCCAGAAAAGTGTGATATTTCGAAAAATTGAGCCAAATCAACGGTTTGGCTGCGCGATCCTG
GCGTCACCAGGGGGCGTGTT

Downstream 100 bases:

>100_bases
ACCAGCACGATTTCATGATTGCGCTGGTCGTCTTCCTGGTGGCGGCAGTGGTGGCCGTGCCGCTGGCGCGGCGATTCGGG
CTTGGCGCCGTGCTCGGATA

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDLLQRRILLLPATRWTRSYPDTMTEPPVVVIFAHPSPRRSRVNRPLAEALAALPHVEVRELYRLYVDYDIDVVAEQRLL
STAETLVLQFPVRWYSVPALLKLWLDDVLESGWAFGPGGTALRGKSMLAVVSTGGHADAYGPEGTHGHPIADFLLPLEQT
ALLCGMTWLPPVVLHDADNADSDAVALHIEHVARHLPALPLPPETQQWTDAELGGEDAP

Sequences:

>Translated_219_residues
MDLLQRRILLLPATRWTRSYPDTMTEPPVVVIFAHPSPRRSRVNRPLAEALAALPHVEVRELYRLYVDYDIDVVAEQRLL
STAETLVLQFPVRWYSVPALLKLWLDDVLESGWAFGPGGTALRGKSMLAVVSTGGHADAYGPEGTHGHPIADFLLPLEQT
ALLCGMTWLPPVVLHDADNADSDAVALHIEHVARHLPALPLPPETQQWTDAELGGEDAP
>Mature_219_residues
MDLLQRRILLLPATRWTRSYPDTMTEPPVVVIFAHPSPRRSRVNRPLAEALAALPHVEVRELYRLYVDYDIDVVAEQRLL
STAETLVLQFPVRWYSVPALLKLWLDDVLESGWAFGPGGTALRGKSMLAVVSTGGHADAYGPEGTHGHPIADFLLPLEQT
ALLCGMTWLPPVVLHDADNADSDAVALHIEHVARHLPALPLPPETQQWTDAELGGEDAP

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4505415, Length=122, Percent_Identity=31.9672131147541, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1786231, Length=187, Percent_Identity=42.7807486631016, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1789750, Length=166, Percent_Identity=40.3614457831325, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 24179; Mature: 24179

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLQRRILLLPATRWTRSYPDTMTEPPVVVIFAHPSPRRSRVNRPLAEALAALPHVEVR
CCHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCHHHH
ELYRLYVDYDIDVVAEQRLLSTAETLVLQFPVRWYSVPALLKLWLDDVLESGWAFGPGGT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ALRGKSMLAVVSTGGHADAYGPEGTHGHPIADFLLPLEQTALLCGMTWLPPVVLHDADNA
EECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEECCCCC
DSDAVALHIEHVARHLPALPLPPETQQWTDAELGGEDAP
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLLQRRILLLPATRWTRSYPDTMTEPPVVVIFAHPSPRRSRVNRPLAEALAALPHVEVR
CCHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCHHHHHHHHCCCHHHH
ELYRLYVDYDIDVVAEQRLLSTAETLVLQFPVRWYSVPALLKLWLDDVLESGWAFGPGGT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ALRGKSMLAVVSTGGHADAYGPEGTHGHPIADFLLPLEQTALLCGMTWLPPVVLHDADNA
EECCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEECCCCC
DSDAVALHIEHVARHLPALPLPPETQQWTDAELGGEDAP
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA