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The map label for this gene is ntaB [H]

Identifier: 94312249

GI number: 94312249

Start: 3581461

End: 3581973

Strand: Reverse

Name: ntaB [H]

Synonym: Rmet_3318

Alternate gene names: 94312249

Gene position: 3581973-3581461 (Counterclockwise)

Preceding gene: 94312250

Following gene: 94312248

Centisome position: 91.19

GC content: 68.03

Gene sequence:

>513_bases
ATGGACTCGACCAAGATAGTCGCAAGCACGCCGGCGGGCGCGCCCGATCCCCGCGCCCTGCGAAGGGTCTGCGGACGGTT
CGCGACGGGCGTGGCGATCATTGGTGCGTGCGCCCCACAAGGCCGGCCGGTGGGGCTGACCGTGAACTCGTTCGCCTCGC
TGTCGCTGGATCCGCCGCTGATCGTTTGGAGCCTGGCGCGCCATTCGCCCAATGCGGCACTGTTTCAGTCAGGAGAGGCG
TTCGGCGTGAGCATCCTCCGGGCCGGGCACGACGAACTGGCGCGTCGATTCGCCAGACCCCAGCCCGACAAGTTCGCCGG
TGTGCCGCACTGGCGCTGTCCGCGTGGCGTGCCATATCTTGACGAGGCGCTCGCGACGCTTTCGTGCCGTGTCGAGCGCG
CCGACCCGCTCGGCGATCACCTGCTGATCGTCGGTGCGGTAGAGGATTTTGCAGCCTCCGATGGTGATCCGCTGGTGTTC
TACGGGGGCGATTTCGTCCGCGTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCCAACGGATTCGACAATCCACCGGATTTCTTCCCGTGGTGGTCCGATCCGTTGGCATGTCAGCAGTTGATCGACCAGCA
ACTGGCCAAGGCAGCGTGAC

Downstream 100 bases:

>100_bases
AATGCACACTTCGGCGGCCATCGCGCCGCCACGAGTTGGCTTTACGCCGCAATTTCGCCGAATTCTGCCGCTACAGACGT
GGTCCTGCGCGGGATTCGGC

Product: putative flavin reductase (conserved protein, domain typically associated with flavoprotein oxygenases)

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPLIVWSLARHSPNAALFQSGEA
FGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYLDEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVF
YGGDFVRVAA

Sequences:

>Translated_170_residues
MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPLIVWSLARHSPNAALFQSGEA
FGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYLDEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVF
YGGDFVRVAA
>Mature_170_residues
MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPLIVWSLARHSPNAALFQSGEA
FGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYLDEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVF
YGGDFVRVAA

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17959; Mature: 17959

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCEECCCCCCE
IVWSLARHSPNAALFQSGEAFGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYL
EEEEECCCCCCHHEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
DEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVFYGGDFVRVAA
HHHHHHHHHEEECCCCCCCEEEEEEECHHHHCCCCCEEEEECCCEEEEEC
>Mature Secondary Structure
MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCEECCCCCCE
IVWSLARHSPNAALFQSGEAFGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYL
EEEEECCCCCCHHEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
DEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVFYGGDFVRVAA
HHHHHHHHHEEECCCCCCCEEEEEEECHHHHCCCCCEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]