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The map label for this gene is hisH [H]

Identifier: 94312175

GI number: 94312175

Start: 3516894

End: 3517547

Strand: Reverse

Name: hisH [H]

Synonym: Rmet_3244

Alternate gene names: 94312175

Gene position: 3517547-3516894 (Counterclockwise)

Preceding gene: 94312176

Following gene: 94312174

Centisome position: 89.55

GC content: 63.15

Gene sequence:

>654_bases
ATGACTACCATAGCAATTGTGGATTACGGCATGGGCAACCTGCGCTCGGTAGCGCAGGCCCTGCGCACGGCGGCGCCGGA
GGCGGATGTCCAGGTTGTGGATCGCCCGGAAGCGATCCGCGCGGCAGACCGCGTGGTCCTGCCGGGGCAGGGCGCGATGC
CTGACTGCATGGGCGCGCTTGGCAATTCCGGCCTGCAGGAGGCGGTACTGGAAGCCGCCGCGACCAAGCCGATGCTGGGC
GTGTGCGTTGGCGAACAGATGCTGTTCGAACGTAGCAGCGAAGCTCGTGCGGGCGAACGCAGCACGCCGGCGCTGGGGCT
GATGCCCGGCGAGGTGATCCGTTTTGAACTGGAAGGCATGACGCAGCCCGACGGCTCGCGCTACAAGGTGCCGCAGATGG
GATGGAACCGGGTACGCCAGTCGAAGCCGCATCCGCTGTGGAATGGCATTCCGGACAACAGCTGGTTTTACTTCGTGCAC
AGCTACTATGTGCACGCGCAGGATCCGGCCCACATCGCCGGCGAGACTGAATATGGCGTCGTGTTTACCAGTGCGGTGGC
ACGCGATAATATTTTCGCCACGCAGTTTCACCCGGAGAAGAGCGCCGCGATGGGTTTGCAGCTATACCGGAATTTCGTGC
ACTGGAATCCGTAA

Upstream 100 bases:

>100_bases
GGATCAATATTGGCACGCGCCTGATGGGTCTAATACTCTCGGCGCTGGCCGTTGAATTCATTGTGGACGGGCTGAAGACA
TTGTTGCCTGTTTTGAAATC

Downstream 100 bases:

>100_bases
AGCGTCAGAAGCACCTGTTACCTGAAGCGCCAGTAATACCGTACAGCGATCACCTTTTCGTTCAGAGCCACCAACCAGAC
TGCGCATCTTTTGCCAAACT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGALGNSGLQEAVLEAAATKPMLG
VCVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGMTQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVH
SYYVHAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP

Sequences:

>Translated_217_residues
MTTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGALGNSGLQEAVLEAAATKPMLG
VCVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGMTQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVH
SYYVHAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
>Mature_216_residues
TTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGALGNSGLQEAVLEAAATKPMLGV
CVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGMTQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVHS
YYVHAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=35.5450236966825, Blast_Score=119, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=225, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23805; Mature: 23674

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGAL
CCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHHH
GNSGLQEAVLEAAATKPMLGVCVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGM
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCEEECCCCEEEEEEECCC
TQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVHSYYVHAQDPAHIAGETEYGV
CCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCE
VFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
EEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGAL
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHHH
GNSGLQEAVLEAAATKPMLGVCVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGM
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCEEECCCCEEEEEEECCC
TQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVHSYYVHAQDPAHIAGETEYGV
CCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCE
VFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP
EEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA