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The map label for this gene is baiB [H]

Identifier: 94312146

GI number: 94312146

Start: 3488529

End: 3490031

Strand: Reverse

Name: baiB [H]

Synonym: Rmet_3215

Alternate gene names: 94312146

Gene position: 3490031-3488529 (Counterclockwise)

Preceding gene: 94312150

Following gene: 94312145

Centisome position: 88.85

GC content: 67.2

Gene sequence:

>1503_bases
ATGGCCGCAGACCTCTGGTTCGAATCGTTGCACCGCACCGGCACCGAAGTGCTCGACCGCGGTAATCGGCTGGCGGGTGG
CCTGCGGCGGCTGGGGCTTGAGGAGGGCGCGGTGGTGGCCGTGATGCTGCGCAATGACCCGGTGTTTGCCGACGTCGTCT
TCGCGTGCCGGACGGCCGGCGTCTACTACTGCCCGATCAACTGGCATTTCACGCCGGAGGAAGCCCGCTTCCTGCTGGAG
GACAGCAGCGCCCGCGTGCTGATCATCGAGGCCGATCTGTTCCCGTCGATGCGCGAAGTGATTCCCGCAGGCATGACCGT
GCTGACCGTGAACGGCAGCGACGCCGATGCCACCATCACCATCGCCTACGAACCCTGGCTCGCCACGCAATCGCCGTACG
ACGGCCCACGCGTTGCGCCACGTGGACATCTGGCCTATACGTCGGGCACCACCGGCCGTCCCAAGGGCGTGCTGCGCAGC
GCGGTGCCGCTGGGTGAACTCGAGGACCAGACCGCGCGCATGCGATCGGTGGTGGCCAAGGCAATCGGGATCGTGCCGGG
GTGCCGTGCGCTGATGTCCGCGCCGCTCTATCACAGCGCGCCCGGTGTCTTTATCCAGAACGCGCTGCAGATGGCCGAGC
GCCTGGTACTGACGCCGCGCTTCGACGCCGAGCAGGTGCTGGCGCTGGTCGAGAAGCATCGCATCGACGTGCTCTACCTG
GTGCCGATCATGTACGTGCGCCTGCTCAAGCTGCCGCCCGAGGTGCGCGCGCGCTACGACCTGTCGTCGATCCGCTTTGT
CGCGTCCACGGGCTCGCCGTGCGCCCCGGAGGTCAAGCGCGCCATGCTCGACTGGTTCGGCCCGGTGATCCACGAAACCT
ATGCGTCGAGCGAGGCGGGCATGGTCACCGTGGCCACACCCGAGGATGCGGCCGAGCGTCCGGGCACGGCGGGGCGTCCG
GTTGATGCGGCCAGCGTGCGCATCATCGACGAATCCGGACGACCCTGCGCGCCAGGCGAAGTCGGGCTCGTGTACGTCCG
CCAGCCCGCCTATCCCGATTTCACCTACCTGAACAACGATGCCGCACGACGGACGATCGACGTGGACGGTCGCGTCACGC
TTGGCGACATGGGGTACCTCGATGCGGATGGCTACCTGTTCATCTGCGACCGCGCGTCGGACATGGTCATCTCGGGTGGC
GTGAACATCTATCCGGCCGAGATCGAGCATGAACTCGTGCGCTATCCCGGAGTGGCCGATTGCGTGGTGTTTGGCGTGCC
GGACGACGAGTATGGCGAGCGGCTCCACGGCGTGATTCAGCCCATGGCCGGCACAACGCTCGATCCGGTGGCCGTGATCG
ACTGGATGCGAGGCCGGCTGTCCGGCTTCAAGGTGCCGCGCACGATCGAAATCGTCGCGCAGTTGCCGCGCGACGAAACG
GGCAAGCTGGCAAAGCGCCGGCTGCGCGACCAGCACTGGGCAGGGCGGCAGCGAAGGGTTTGA

Upstream 100 bases:

>100_bases
AGTTTGACGCAACATTGTGATTCCAGTCGGAGGCATTGTCGATGCCAGTAGGCACCGCTACAGTGTTCGGTCAACAAAAT
GCGCAGACAGGAGACCCCGC

Downstream 100 bases:

>100_bases
TGGAAGCCGGCCGCGACGGCGACGGCGTCGGCCACTGACAGGCTCCGCTTTCCCGCTTTGCGGGGGAGGGGAGACAACAC
ACATAAAACACAAAGCAGCA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAADLWFESLHRTGTEVLDRGNRLAGGLRRLGLEEGAVVAVMLRNDPVFADVVFACRTAGVYYCPINWHFTPEEARFLLE
DSSARVLIIEADLFPSMREVIPAGMTVLTVNGSDADATITIAYEPWLATQSPYDGPRVAPRGHLAYTSGTTGRPKGVLRS
AVPLGELEDQTARMRSVVAKAIGIVPGCRALMSAPLYHSAPGVFIQNALQMAERLVLTPRFDAEQVLALVEKHRIDVLYL
VPIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCAPEVKRAMLDWFGPVIHETYASSEAGMVTVATPEDAAERPGTAGRP
VDAASVRIIDESGRPCAPGEVGLVYVRQPAYPDFTYLNNDAARRTIDVDGRVTLGDMGYLDADGYLFICDRASDMVISGG
VNIYPAEIEHELVRYPGVADCVVFGVPDDEYGERLHGVIQPMAGTTLDPVAVIDWMRGRLSGFKVPRTIEIVAQLPRDET
GKLAKRRLRDQHWAGRQRRV

Sequences:

>Translated_500_residues
MAADLWFESLHRTGTEVLDRGNRLAGGLRRLGLEEGAVVAVMLRNDPVFADVVFACRTAGVYYCPINWHFTPEEARFLLE
DSSARVLIIEADLFPSMREVIPAGMTVLTVNGSDADATITIAYEPWLATQSPYDGPRVAPRGHLAYTSGTTGRPKGVLRS
AVPLGELEDQTARMRSVVAKAIGIVPGCRALMSAPLYHSAPGVFIQNALQMAERLVLTPRFDAEQVLALVEKHRIDVLYL
VPIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCAPEVKRAMLDWFGPVIHETYASSEAGMVTVATPEDAAERPGTAGRP
VDAASVRIIDESGRPCAPGEVGLVYVRQPAYPDFTYLNNDAARRTIDVDGRVTLGDMGYLDADGYLFICDRASDMVISGG
VNIYPAEIEHELVRYPGVADCVVFGVPDDEYGERLHGVIQPMAGTTLDPVAVIDWMRGRLSGFKVPRTIEIVAQLPRDET
GKLAKRRLRDQHWAGRQRRV
>Mature_499_residues
AADLWFESLHRTGTEVLDRGNRLAGGLRRLGLEEGAVVAVMLRNDPVFADVVFACRTAGVYYCPINWHFTPEEARFLLED
SSARVLIIEADLFPSMREVIPAGMTVLTVNGSDADATITIAYEPWLATQSPYDGPRVAPRGHLAYTSGTTGRPKGVLRSA
VPLGELEDQTARMRSVVAKAIGIVPGCRALMSAPLYHSAPGVFIQNALQMAERLVLTPRFDAEQVLALVEKHRIDVLYLV
PIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCAPEVKRAMLDWFGPVIHETYASSEAGMVTVATPEDAAERPGTAGRPV
DAASVRIIDESGRPCAPGEVGLVYVRQPAYPDFTYLNNDAARRTIDVDGRVTLGDMGYLDADGYLFICDRASDMVISGGV
NIYPAEIEHELVRYPGVADCVVFGVPDDEYGERLHGVIQPMAGTTLDPVAVIDWMRGRLSGFKVPRTIEIVAQLPRDETG
KLAKRRLRDQHWAGRQRRV

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI122937307, Length=500, Percent_Identity=26.4, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=25.9842519685039, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI156151445, Length=530, Percent_Identity=24.9056603773585, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=22.7099236641221, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI157311624, Length=500, Percent_Identity=26.2, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157311622, Length=500, Percent_Identity=26.2, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI58082049, Length=506, Percent_Identity=25.8893280632411, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI187761345, Length=522, Percent_Identity=25.4789272030651, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI187761343, Length=522, Percent_Identity=25.4789272030651, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=24.0157480314961, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=25, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI253970489, Length=367, Percent_Identity=24.7956403269755, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI8923896, Length=367, Percent_Identity=24.7956403269755, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI13375727, Length=526, Percent_Identity=23.1939163498099, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62865631, Length=519, Percent_Identity=24.2774566473988, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI13325055, Length=519, Percent_Identity=24.2774566473988, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI38524616, Length=515, Percent_Identity=24.6601941747573, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI195947378, Length=388, Percent_Identity=25.5154639175258, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=26.1296660117878, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=24.2362525458248, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=24.1758241758242, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693145, Length=488, Percent_Identity=25.2049180327869, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786810, Length=500, Percent_Identity=25.2, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786529, Length=531, Percent_Identity=24.6704331450094, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=484, Percent_Identity=28.099173553719, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71994694, Length=536, Percent_Identity=26.1194029850746, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=536, Percent_Identity=26.1194029850746, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=536, Percent_Identity=26.1194029850746, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=490, Percent_Identity=26.1224489795918, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=487, Percent_Identity=24.8459958932238, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=455, Percent_Identity=27.2527472527473, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=471, Percent_Identity=25.0530785562633, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=495, Percent_Identity=24.8484848484848, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=485, Percent_Identity=24.1237113402062, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=486, Percent_Identity=20.1646090534979, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=489, Percent_Identity=20.6543967280164, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=355, Percent_Identity=23.6619718309859, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=523, Percent_Identity=22.9445506692161, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=523, Percent_Identity=22.9445506692161, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=27.1468144044321, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=536, Percent_Identity=23.134328358209, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6319264, Length=383, Percent_Identity=26.6318537859008, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922652, Length=436, Percent_Identity=27.0642201834862, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24648260, Length=492, Percent_Identity=25, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21358303, Length=464, Percent_Identity=25, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355181, Length=482, Percent_Identity=24.896265560166, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24648253, Length=486, Percent_Identity=23.8683127572016, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281365686, Length=456, Percent_Identity=26.5350877192982, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648255, Length=503, Percent_Identity=23.6580516898608, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356441, Length=486, Percent_Identity=22.2222222222222, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=23.2464929859719, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24581924, Length=521, Percent_Identity=24.1842610364683, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648257, Length=486, Percent_Identity=23.4567901234568, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18859661, Length=483, Percent_Identity=23.8095238095238, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667959, Length=391, Percent_Identity=25.0639386189258, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI62472339, Length=391, Percent_Identity=25.0639386189258, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24667955, Length=391, Percent_Identity=25.0639386189258, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653035, Length=354, Percent_Identity=24.5762711864407, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=21.4428857715431, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161076582, Length=367, Percent_Identity=24.2506811989101, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355225, Length=376, Percent_Identity=24.2021276595745, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28573631, Length=533, Percent_Identity=23.6397748592871, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI20130353, Length=533, Percent_Identity=23.6397748592871, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20130357, Length=487, Percent_Identity=21.5605749486653, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 54874; Mature: 54742

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADLWFESLHRTGTEVLDRGNRLAGGLRRLGLEEGAVVAVMLRNDPVFADVVFACRTAG
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCC
VYYCPINWHFTPEEARFLLEDSSARVLIIEADLFPSMREVIPAGMTVLTVNGSDADATIT
EEEECCEEEECHHHHHEEEECCCCEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCEEEE
IAYEPWLATQSPYDGPRVAPRGHLAYTSGTTGRPKGVLRSAVPLGELEDQTARMRSVVAK
EEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
AIGIVPGCRALMSAPLYHSAPGVFIQNALQMAERLVLTPRFDAEQVLALVEKHRIDVLYL
HHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCEEHH
VPIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCAPEVKRAMLDWFGPVIHETYASSEAG
HHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
MVTVATPEDAAERPGTAGRPVDAASVRIIDESGRPCAPGEVGLVYVRQPAYPDFTYLNND
EEEEECCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEECCC
AARRTIDVDGRVTLGDMGYLDADGYLFICDRASDMVISGGVNIYPAEIEHELVRYPGVAD
HHCEEEECCCEEEECCCCEECCCCEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCC
CVVFGVPDDEYGERLHGVIQPMAGTTLDPVAVIDWMRGRLSGFKVPRTIEIVAQLPRDET
EEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHCCCCCH
GKLAKRRLRDQHWAGRQRRV
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AADLWFESLHRTGTEVLDRGNRLAGGLRRLGLEEGAVVAVMLRNDPVFADVVFACRTAG
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCC
VYYCPINWHFTPEEARFLLEDSSARVLIIEADLFPSMREVIPAGMTVLTVNGSDADATIT
EEEECCEEEECHHHHHEEEECCCCEEEEEECCCCHHHHHHHCCCEEEEEEECCCCCEEEE
IAYEPWLATQSPYDGPRVAPRGHLAYTSGTTGRPKGVLRSAVPLGELEDQTARMRSVVAK
EEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
AIGIVPGCRALMSAPLYHSAPGVFIQNALQMAERLVLTPRFDAEQVLALVEKHRIDVLYL
HHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCEEHH
VPIMYVRLLKLPPEVRARYDLSSIRFVASTGSPCAPEVKRAMLDWFGPVIHETYASSEAG
HHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
MVTVATPEDAAERPGTAGRPVDAASVRIIDESGRPCAPGEVGLVYVRQPAYPDFTYLNND
EEEEECCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCEEECCC
AARRTIDVDGRVTLGDMGYLDADGYLFICDRASDMVISGGVNIYPAEIEHELVRYPGVAD
HHCEEEECCCEEEECCCCEECCCCEEEEECCCCCEEEECCCEEEEHHHHHHHHHCCCCCC
CVVFGVPDDEYGERLHGVIQPMAGTTLDPVAVIDWMRGRLSGFKVPRTIEIVAQLPRDET
EEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHEEHHHHCCCCCH
GKLAKRRLRDQHWAGRQRRV
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]