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The map label for this gene is clcA [H]

Identifier: 94312144

GI number: 94312144

Start: 3485617

End: 3487185

Strand: Reverse

Name: clcA [H]

Synonym: Rmet_3213

Alternate gene names: 94312144

Gene position: 3487185-3485617 (Counterclockwise)

Preceding gene: 94312145

Following gene: 335055657

Centisome position: 88.78

GC content: 66.67

Gene sequence:

>1569_bases
ATGCCTCCGGTAGCCTCTGTCATCTTCCCCCGCTGCCAACGCTCACGCATCGACGCCATGACCGATTCCGCCGAGCCGCC
CGATTCGTCCAACTCTTCCGGTTCCTCCGATCCCACCAAAGCCACGTCGCCTTCTGGTGCCGGCAAGCCCCCAGATTCCG
CATCCACGCGACACCCGGACCAGCCCGACCGCCTGAGCAAGTTGCGCTTGCACGCGCGCAGCACCGCATTCCGCAAGACA
CGTCAGTTTGGCCGAATTTCGCGCACCACCATTCGCTATGGTGCGTTCATGTGCGGCGCGGGCTTCGTGGCGTTGTTCTC
GATCGTCTTTGCCTATATCGCCGAAGTGGCGCTGAACTGGAACGCGAAGCTGACGACGGCCACGCCGTGGCTGGCGTTCG
TGATCCTGCCGTTCGGGCTGGCCGCATTGCGCTGGCTGACCATTCGGCTGGCGCCGCAGGCGCGCGGCAGCGGCATTCCG
CAGGTGATCGCCGCCGTGACGCTGCCGCCTGCGGGCGTTGCGCAGACCGTGCTGGTCTCGTTCCGGCAATCCATGTGGAA
GGTGTTGCTGACGACCGGCGCATTGCTCGCCGGCGCCTCGGTGGGCCGTGAAGGGCCATCGGTTCAGGTCGGCGCCGCTG
CCATGCTGGCCTGGGGCCGCTTGTGTCACGAGAAGCTCCAGTTTCGCATCGGATTTCATCCGAACGCGCTGATCGCGGCC
GGTGCGGCCGGTGGTCTTGCCGCGGCATTCAACACGCCGCTGGCCGGGGTGGTGTTCGCGATCGAGGAATTGGGCCGTGG
AACGTCGGTGCGTTGGGACAGGCTGGTGCTATCCGGCGTGCTGACGGCAGGCTTTCTGTCGCTGGCAGTGCTCGGCAACA
ATCCGTACTTCAGCGTCAAGGTGCCGATGCTGGTGCTGCACGACGCCTGGGGGCCGGTGCTGCTCTGCGCCGTGGTCAAC
GGCGTGATGGGCGGGCTGTTCGCCAAGCTGCTGATCGCGGGGGTGCCGGGCCTGACACCGTCATCGTGGCGCGGCTGGCT
GAACGCGCATCCGGTCATGGTGGCGTTCTTCTGCGGCCTGATCGTGGCCGCGCTCGGCTGGGCAACCGCTGGCGCGACGT
TCGGCACGGGCTATGAGCAGGCATCTGGCCTGATCAACGGCACGCCACACAGCACGATCTGGTTCGGGCTGGCGAAGTTC
GTTGCCACCGTGGTGTCGTATTTCGCGGGCATCCCTGGTGGCATCTTTACCCCCTCGCTGGCCATCGGCGCGGGCATCGG
CGACACCGTGTCCCATTTCCTCTCGGGCGCGACCGAGCCGCGCGTGCTGGCGCTGGTGTCGATGGCCGCATTCCTGGCCG
CCGCCACGCAGGCGCCGATCACCGCCAGCGTGATCGTGATGGAGATGACCCGCACGCAGGATCTGACGATCTACCTGCTT
GCGGCGTCGCTGCTGGCGTCGTTCCTGTCGCGCCAGTTCAACCCGCATCCGTTCTATCACCACATGGGCCACGCCTTCCG
CCGCGAGGCAATGTCGATCGGCCGGAAGTCAGCGTCAGCGTCCGCGTGA

Upstream 100 bases:

>100_bases
CCAGAACCGCTTCGCTTCATTGTGATGGCGCCTCTTAGCCTGAATCCTGGGGGACAGCGGGAGCGACGGGCGTATAGTTG
AGGGCTGCCGGAGGCGCTGG

Downstream 100 bases:

>100_bases
CGCGTGGTTGCCACCGCTGCCAGAGGAAAGGCGCAGTGGTGGCACGGTGGAGCCGCTGTGGCGGACACGGCCACTTTGTC
TTAACATGTGCCGCGCGTGG

Product: Cl-channel, voltage gated

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MPPVASVIFPRCQRSRIDAMTDSAEPPDSSNSSGSSDPTKATSPSGAGKPPDSASTRHPDQPDRLSKLRLHARSTAFRKT
RQFGRISRTTIRYGAFMCGAGFVALFSIVFAYIAEVALNWNAKLTTATPWLAFVILPFGLAALRWLTIRLAPQARGSGIP
QVIAAVTLPPAGVAQTVLVSFRQSMWKVLLTTGALLAGASVGREGPSVQVGAAAMLAWGRLCHEKLQFRIGFHPNALIAA
GAAGGLAAAFNTPLAGVVFAIEELGRGTSVRWDRLVLSGVLTAGFLSLAVLGNNPYFSVKVPMLVLHDAWGPVLLCAVVN
GVMGGLFAKLLIAGVPGLTPSSWRGWLNAHPVMVAFFCGLIVAALGWATAGATFGTGYEQASGLINGTPHSTIWFGLAKF
VATVVSYFAGIPGGIFTPSLAIGAGIGDTVSHFLSGATEPRVLALVSMAAFLAAATQAPITASVIVMEMTRTQDLTIYLL
AASLLASFLSRQFNPHPFYHHMGHAFRREAMSIGRKSASASA

Sequences:

>Translated_522_residues
MPPVASVIFPRCQRSRIDAMTDSAEPPDSSNSSGSSDPTKATSPSGAGKPPDSASTRHPDQPDRLSKLRLHARSTAFRKT
RQFGRISRTTIRYGAFMCGAGFVALFSIVFAYIAEVALNWNAKLTTATPWLAFVILPFGLAALRWLTIRLAPQARGSGIP
QVIAAVTLPPAGVAQTVLVSFRQSMWKVLLTTGALLAGASVGREGPSVQVGAAAMLAWGRLCHEKLQFRIGFHPNALIAA
GAAGGLAAAFNTPLAGVVFAIEELGRGTSVRWDRLVLSGVLTAGFLSLAVLGNNPYFSVKVPMLVLHDAWGPVLLCAVVN
GVMGGLFAKLLIAGVPGLTPSSWRGWLNAHPVMVAFFCGLIVAALGWATAGATFGTGYEQASGLINGTPHSTIWFGLAKF
VATVVSYFAGIPGGIFTPSLAIGAGIGDTVSHFLSGATEPRVLALVSMAAFLAAATQAPITASVIVMEMTRTQDLTIYLL
AASLLASFLSRQFNPHPFYHHMGHAFRREAMSIGRKSASASA
>Mature_521_residues
PPVASVIFPRCQRSRIDAMTDSAEPPDSSNSSGSSDPTKATSPSGAGKPPDSASTRHPDQPDRLSKLRLHARSTAFRKTR
QFGRISRTTIRYGAFMCGAGFVALFSIVFAYIAEVALNWNAKLTTATPWLAFVILPFGLAALRWLTIRLAPQARGSGIPQ
VIAAVTLPPAGVAQTVLVSFRQSMWKVLLTTGALLAGASVGREGPSVQVGAAAMLAWGRLCHEKLQFRIGFHPNALIAAG
AAGGLAAAFNTPLAGVVFAIEELGRGTSVRWDRLVLSGVLTAGFLSLAVLGNNPYFSVKVPMLVLHDAWGPVLLCAVVNG
VMGGLFAKLLIAGVPGLTPSSWRGWLNAHPVMVAFFCGLIVAALGWATAGATFGTGYEQASGLINGTPHSTIWFGLAKFV
ATVVSYFAGIPGGIFTPSLAIGAGIGDTVSHFLSGATEPRVLALVSMAAFLAAATQAPITASVIVMEMTRTQDLTIYLLA
ASLLASFLSRQFNPHPFYHHMGHAFRREAMSIGRKSASASA

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=393, Percent_Identity=29.2620865139949, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081943, Length=194, Percent_Identity=34.020618556701, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 54767; Mature: 54636

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVASVIFPRCQRSRIDAMTDSAEPPDSSNSSGSSDPTKATSPSGAGKPPDSASTRHPD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPDRLSKLRLHARSTAFRKTRQFGRISRTTIRYGAFMCGAGFVALFSIVFAYIAEVALNW
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKLTTATPWLAFVILPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGVAQTVLVS
CCEEEECHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
FRQSMWKVLLTTGALLAGASVGREGPSVQVGAAAMLAWGRLCHEKLQFRIGFHPNALIAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEEC
GAAGGLAAAFNTPLAGVVFAIEELGRGTSVRWDRLVLSGVLTAGFLSLAVLGNNPYFSVK
CCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEE
VPMLVLHDAWGPVLLCAVVNGVMGGLFAKLLIAGVPGLTPSSWRGWLNAHPVMVAFFCGL
ECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHH
IVAALGWATAGATFGTGYEQASGLINGTPHSTIWFGLAKFVATVVSYFAGIPGGIFTPSL
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AIGAGIGDTVSHFLSGATEPRVLALVSMAAFLAAATQAPITASVIVMEMTRTQDLTIYLL
HHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCHHHHHH
AASLLASFLSRQFNPHPFYHHMGHAFRREAMSIGRKSASASA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PPVASVIFPRCQRSRIDAMTDSAEPPDSSNSSGSSDPTKATSPSGAGKPPDSASTRHPD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPDRLSKLRLHARSTAFRKTRQFGRISRTTIRYGAFMCGAGFVALFSIVFAYIAEVALNW
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKLTTATPWLAFVILPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGVAQTVLVS
CCEEEECHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
FRQSMWKVLLTTGALLAGASVGREGPSVQVGAAAMLAWGRLCHEKLQFRIGFHPNALIAA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEEC
GAAGGLAAAFNTPLAGVVFAIEELGRGTSVRWDRLVLSGVLTAGFLSLAVLGNNPYFSVK
CCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEE
VPMLVLHDAWGPVLLCAVVNGVMGGLFAKLLIAGVPGLTPSSWRGWLNAHPVMVAFFCGL
ECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHH
IVAALGWATAGATFGTGYEQASGLINGTPHSTIWFGLAKFVATVVSYFAGIPGGIFTPSL
HHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AIGAGIGDTVSHFLSGATEPRVLALVSMAAFLAAATQAPITASVIVMEMTRTQDLTIYLL
HHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCHHHHHH
AASLLASFLSRQFNPHPFYHHMGHAFRREAMSIGRKSASASA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA