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The map label for this gene is murF [H]

Identifier: 94312063

GI number: 94312063

Start: 3395661

End: 3397061

Strand: Reverse

Name: murF [H]

Synonym: Rmet_3132

Alternate gene names: 94312063

Gene position: 3397061-3395661 (Counterclockwise)

Preceding gene: 94312064

Following gene: 94312062

Centisome position: 86.48

GC content: 67.31

Gene sequence:

>1401_bases
ATGAACCGCCAACCGATGACGACTTTGCAGCAAGCCGCTGGCTGGATTGCCGGCGCAAGTATTTCCGGAGATGCTACGTG
CTCGTTTTCGCGCGTGCATACCGACAGCCGTACCGTCGAGCCCGGCGACCTGTTCGTCGCGCTCAAGGGTGAGCGTTTCG
ACGCCCACGATTTTCTGGCCGATGTGGTCGCGCGCGGCGCCGCCGCGGTGATGGTGAGCCGCAAGGTCGACGTCGACGTG
CCGGCGCTGATCGTGCCCGACACGCGCACCGGCCTCGGCCAGTTGGCCACGGGTTGGCGCCGCACGTTCACGCCGCCGAC
GGTGGCCGTGACCGGCAGCAATGGCAAGACCACGGTCAAGGAAATGATCGCGGCGATCTTTGCCGCGGCCGTGGGTGAAG
GCGATCGTCTGGCAACGGGTGGCAACCTGAACAACGACATCGGCCTGCCACTGACCGTGCTGCGCCTGCGCGCGCATCAC
AAGCTGGCCGTGCTCGAGCTGGGCATGAATCACCCGGGTGAGACGATCTACCTGGCCGGCATCGCCCAGCCGACCGTGGC
GGTGGTCACCAACGCCCAGCGCGAGCACCAGGAGTTCATGGTCAGCGTGGAGGCCGTGGCGCATGAACACGCCAGCGCCA
TCGCCGCGCTGCCCGCCGATGGCGTGGCCGTGTTTCCGGTCGATGCCGAAAGCGGTGGCGCCTATGCGGCCATCTGGCGC
CAGGCCGCTGGCACACGGAAGGTGCTGTCGTTTGGGACCGATGCTTCGGCCGACGTACGGGCTACGGTGTCGCTGGAGAA
TGGCGTGCAGGTGCTGGATGTGACCGCGCCGGACCGGCGCTTCACGCTGCGCCTGCATGTGCTGGGTGTGCACAACGTGC
GCAACGCGCTGGCGGCTACGGCGTGCGCGCTGGCCGCAGGCGTCGATGTGGCGGCAATCCAGGCGGGCATCGCCGGCTTC
ACCGCCGTGAAGGGCCGCCTGCAGGTGAAGCACACCCCGGCGGGCACCGTCGTTATCGACGACACGTACAACGCCAACCC
GGATTCGATGCGTGCTGCAATCGACGTGCTGGCCGGATTCGCGACCCCGCGCCTGCTGGTGCTGGGCGATATGGGCGAGG
TAGGGGATCAGGGTCCGATGTTCCACACCGAGATCGGTGCGTACGCGCGCGAGCGCGGCATCGACGCGCTCTGGGCAACA
GGCGAACTCGCACCGCACGCGGTGCAGGCATATGGCGGTGGCGCACGGTATTTCGCTCGCGCGGAAGACCTGGCGCAGGC
GCTGGAGGCAGACAAGGAGGGCACGGTGGCCAATGCAGCGGCCGTGCTGGTGAAGGGATCGCGCTTCATGCGCATGGAAC
GGATGGTCGATGCGCTTGTCGCATCACCTTCCGCTCATTAA

Upstream 100 bases:

>100_bases
GCAAGGGCCACGAAGCCACGCAGGAGATCCAGGGTCGCAAGCGCCCGTTCTCTGACCGCGAGCACGTGCGTCTGACCCTG
GGCGCGCGGGGGGTGTCGGC

Downstream 100 bases:

>100_bases
CGAAACAGACCAACGATGTTATTGACACTGGCCCAGTGGCTGCAGAACGACTACAGCTTCCTGCGGGTCGTCAACTATCT
GACTTTCCGCGCGGTGATGG

Product: UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLADVVARGAAAVMVSRKVDVDV
PALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVKEMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHH
KLAVLELGMNHPGETIYLAGIAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWR
QAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAATACALAAGVDVAAIQAGIAGF
TAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGFATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWAT
GELAPHAVQAYGGGARYFARAEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH

Sequences:

>Translated_466_residues
MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLADVVARGAAAVMVSRKVDVDV
PALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVKEMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHH
KLAVLELGMNHPGETIYLAGIAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWR
QAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAATACALAAGVDVAAIQAGIAGF
TAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGFATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWAT
GELAPHAVQAYGGGARYFARAEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH
>Mature_466_residues
MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLADVVARGAAAVMVSRKVDVDV
PALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVKEMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHH
KLAVLELGMNHPGETIYLAGIAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWR
QAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAATACALAAGVDVAAIQAGIAGF
TAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGFATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWAT
GELAPHAVQAYGGGARYFARAEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=459, Percent_Identity=40.0871459694989, Blast_Score=264, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48629; Mature: 48629

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLA
CCCCCHHHHHHHCCEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHH
DVVARGAAAVMVSRKVDVDVPALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVK
HHHHCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHCCCHHCCCCCEEEEECCCCCHHHH
EMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHHKLAVLELGMNHPGETIYLAG
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCEEEEEE
IAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWR
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCEEHHHHH
QAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAAT
HCCCCHHHEECCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEEEEHHHHHHHHHHH
ACALAAGVDVAAIQAGIAGFTAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGF
HHHHHHCCCHHHHHHCHHHHEEECCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHC
ATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWATGELAPHAVQAYGGGARYFAR
CCCEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCHHHHHH
AEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH
HHHHHHHHHCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLA
CCCCCHHHHHHHCCEEECCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHH
DVVARGAAAVMVSRKVDVDVPALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVK
HHHHCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHCCCHHCCCCCEEEEECCCCCHHHH
EMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHHKLAVLELGMNHPGETIYLAG
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCEEEEEE
IAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWR
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCEEHHHHH
QAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAAT
HCCCCHHHEECCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEEEEHHHHHHHHHHH
ACALAAGVDVAAIQAGIAGFTAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGF
HHHHHHCCCHHHHHHCHHHHEEECCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHC
ATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWATGELAPHAVQAYGGGARYFAR
CCCEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCCHHHHHH
AEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH
HHHHHHHHHCCCCCCHHHEEEEEEECHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]