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The map label for this gene is murD

Identifier: 94312061

GI number: 94312061

Start: 3392911

End: 3394425

Strand: Reverse

Name: murD

Synonym: Rmet_3130

Alternate gene names: 94312061

Gene position: 3394425-3392911 (Counterclockwise)

Preceding gene: 94312062

Following gene: 94312060

Centisome position: 86.41

GC content: 67.92

Gene sequence:

>1515_bases
GTGTTTGGCGTGTTGCAGAAACCTCATGTGCTGGTACTTGGCCTCGGTGAATCGGGGCTGGCCATGGCACGCTGGTGCGG
CCTGAATGGCTGCCGCGTGCGCGTGGCCGACACACGCGAGGCGCCCGCCAACCTTGTCTTCCTGCAGGCGGAACTGACCA
CGGCCCAGTTCATGGGCGGGCAGTTCACCGAGAACCTGCTCGACGATATCGGTCTGGTGGCGATCAGCCCCGGGCTGTCC
CCGCTCGAACCCAATACCAGGGCGCTGCTCGAGGCGGCGCAGGCACGCAGTATTCCTGTCTGGGGCGAGATCGAACTGTT
CGCCCAGGCGATTGGCTACCTGGAGGCCACGTCGGGCTACGCCCCGCGCGTGCTGGCCATCACCGGTACCAACGGCAAGA
CCACGACCACCGCGCTGACTGGCCGGCTGATCGAGCGCGCAGGCAAGACCGTGGGCGTGGCAGGCAATATCAGCCCGTCT
GCGCTGGACAAGCTGTCGGCCTGCATCGCCAGCGCCACGTTGCCCGATGTCTGGGTGCTCGAACTGTCCAGCTTCCAGCT
CGAATACACGTTCTCGCTGGCGCCGCACGCGGCCACGGTGCTCAATGTCACGCAGGACCACCTCGACTGGCACGGCAGCA
TGGAAGCGTATGCCGCCGCCAAGGCGCGCATCTTCGGCCCGGCCGAGAAGGGCTGCCTGCAGGTGCTCAACCGGCAGGAC
CCGCTGACGATGAACATGGCCCGCCGCGGCACCACGCTGGTCACGTTCGGCACTGACCTGCCCGAGACGCCGGGCAGCTA
CGGCGTGCTGCGCGAAGGCGGGATGCCATGGCTCGTGCTGGCCGAGCCCGACACCGAAGCCGATGCCGAGCAGAAGCCGC
GCCGCCGCAAGAAGGACGATGTCGCCGCCGACGCCGTCGTGCCGGTACGCCACAAGCGCCTGATGCCGGCCGACGCGCTG
CACATCCGCGGCATGCACAACGCCACCAACGCGATGGCCGCGCTGGCGCTGTGCCGCGCGATCGACCTGCCGCTCAACGC
GCTGCTGCACGGCCTGCGCGAGTATCGTGGCGAGCCGCACCGCGTGGAATGGGTGGCAACGATCGATGAAGTCGAATACT
TCGACGACAGCAAGGGGACGAACGTCGGCGCCACCGTGGCTGCACTGAGCGGGCTGGATAAGCACGTGGTGCTGATCGCC
GGTGGCGAGGGCAAGGGCCAGGATTTCTCGCCGCTGGTCGCGCCGGTGGCGCAATACGCGCGCGCCGTGGTGCTGATCGG
CAAGGATGCGGGCGCGCTGCGCGAGGCGCTGGGGGCTACCGGCAAACCGCTGATCGACGCCGGCTCGCTGGAAGAAGCGG
TAGAGAAATCCGCCAGCCTCGCGGAAGCGGGCGACGTGGTGCTGCTGTCGCCGGCTTGCGCCAGCCTCGACATGTTCCGT
AACTACGTGCATCGCGCCCAGGTGTTCCGCGGCGCGGTGGAAGAACTGGCTTTGTCCCGGGGGATCATGCCATGA

Upstream 100 bases:

>100_bases
TCACGATGCTGCTGGTGCTGATCGGGTTGTCGTCGCTGAAGCTGCGCTGAACCCGGGCTGAAATTTTTAATCTACGGACT
CTACAAGGCAGGAAAACGAA

Downstream 100 bases:

>100_bases
GCGACTCGCAGGTCAAGCGCAGCGGATTCATCGGCGCCACCATCGGCAATGCCTGGGGTGGCCTGCGTGATGCGGTGTCC
GGCGTCAAGCCGACACGCTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGGQFTENLLDDIGLVAISPGLS
PLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGYAPRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQD
PLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDDVAADAVVPVRHKRLMPADAL
HIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIA
GGEGKGQDFSPLVAPVAQYARAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFR
NYVHRAQVFRGAVEELALSRGIMP

Sequences:

>Translated_504_residues
MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGGQFTENLLDDIGLVAISPGLS
PLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGYAPRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQD
PLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDDVAADAVVPVRHKRLMPADAL
HIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIA
GGEGKGQDFSPLVAPVAQYARAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFR
NYVHRAQVFRGAVEELALSRGIMP
>Mature_504_residues
MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGGQFTENLLDDIGLVAISPGLS
PLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGYAPRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQD
PLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDDVAADAVVPVRHKRLMPADAL
HIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIA
GGEGKGQDFSPLVAPVAQYARAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFR
NYVHRAQVFRGAVEELALSRGIMP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=497, Percent_Identity=35.4124748490946, Blast_Score=211, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_RALME (Q1LIM4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585271.1
- ProteinModelPortal:   Q1LIM4
- SMR:   Q1LIM4
- STRING:   Q1LIM4
- GeneID:   4039959
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3130
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q1LIM4
- ProtClustDB:   PRK02006
- BioCyc:   RMET266264:RMET_3130-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 53564; Mature: 53564

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGG
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHCCC
QFTENLLDDIGLVAISPGLSPLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGY
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
APRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPSALDKLSACIASATLPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQD
EECCEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHHCCCC
PLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDD
CCEEEHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHCCCC
VAADAVVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPH
CCCCCCCCHHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIAGGEGKGQDFSPLVAPVAQYA
EEEEEEEEHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
RAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFR
CEEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
NYVHRAQVFRGAVEELALSRGIMP
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGG
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHCCC
QFTENLLDDIGLVAISPGLSPLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGY
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
APRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPSALDKLSACIASATLPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQD
EECCEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHHCCCC
PLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDD
CCEEEHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHCCCC
VAADAVVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPH
CCCCCCCCHHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
RVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIAGGEGKGQDFSPLVAPVAQYA
EEEEEEEEHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHC
RAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFR
CEEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
NYVHRAQVFRGAVEELALSRGIMP
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA