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The map label for this gene is ampD [H]

Identifier: 94312020

GI number: 94312020

Start: 3346969

End: 3347571

Strand: Reverse

Name: ampD [H]

Synonym: Rmet_3089

Alternate gene names: 94312020

Gene position: 3347571-3346969 (Counterclockwise)

Preceding gene: 94312021

Following gene: 94312019

Centisome position: 85.22

GC content: 66.83

Gene sequence:

>603_bases
ATGTCTGACGCCGCAGGCCGGGTCCGCGACTGGCGGCCCAACGCCGACGGCTGGGTCCCCGCAGCCCGCCACGTGCCGTC
CCCGAACTTCGACGCCCGCCCCGAAGGCATGGCGGTCGATGTGGTGGTACTGCACAACATCAGCCTGCCGCCGGGGCAGT
TCGGCTCCGACGATATCGAGGCGTTCTTCCAGAATCGGCTCGATGCCACGCGCCATCCGTTTTTCGAGACGATTCGCGAT
GTCCGGGTATCGGCCCACTTCCTGATCCGTCGCGATGGCGAGCCGGTTCAGTTTGTCGCCTGCACACAACGTGCGTGGCA
CGCCGGCCAGTCCGACTATTGCGGACGCCAACGCTGCAACGACTTCTCGATCGGCATCGAGATCGAGGGCACCGACGACC
AGCCGTTCACGCCAGCTCAGTACGAGACGGTCGCCGCGCTGGTGAGTGCCCTGCGGGCCGCCTATCCGATCCAGGGCGTC
GCTGGCCACAGCGACATTGCTCCGGGACGCAAGACCGACCCAGGCCCCTACTTCGACTGGAGCCGCTTTGCGAAACAGGC
CGCGCTGCCGCTGGCGATGATGCCGTACCGGCAGGCAAGGTAG

Upstream 100 bases:

>100_bases
CGCCAGTTGCGGTATCGGATCGAACAGCTCGGTATCTGCGAGGAGGATGGGCGCAGCGGCCGGGACGATAGCGAATCGCT
GGCCGAAGGGGAGGGGCGCG

Downstream 100 bases:

>100_bases
GTTTTCCAGGGGTGTCAAATAGGGTGCGCCGGGCCCCGAATCGTCACGACGGCTGCCACATTCTGGGACACCTATCGCCG
ACCTGTTCCCATAGAAATAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSDAAGRVRDWRPNADGWVPAARHVPSPNFDARPEGMAVDVVVLHNISLPPGQFGSDDIEAFFQNRLDATRHPFFETIRD
VRVSAHFLIRRDGEPVQFVACTQRAWHAGQSDYCGRQRCNDFSIGIEIEGTDDQPFTPAQYETVAALVSALRAAYPIQGV
AGHSDIAPGRKTDPGPYFDWSRFAKQAALPLAMMPYRQAR

Sequences:

>Translated_200_residues
MSDAAGRVRDWRPNADGWVPAARHVPSPNFDARPEGMAVDVVVLHNISLPPGQFGSDDIEAFFQNRLDATRHPFFETIRD
VRVSAHFLIRRDGEPVQFVACTQRAWHAGQSDYCGRQRCNDFSIGIEIEGTDDQPFTPAQYETVAALVSALRAAYPIQGV
AGHSDIAPGRKTDPGPYFDWSRFAKQAALPLAMMPYRQAR
>Mature_199_residues
SDAAGRVRDWRPNADGWVPAARHVPSPNFDARPEGMAVDVVVLHNISLPPGQFGSDDIEAFFQNRLDATRHPFFETIRDV
RVSAHFLIRRDGEPVQFVACTQRAWHAGQSDYCGRQRCNDFSIGIEIEGTDDQPFTPAQYETVAALVSALRAAYPIQGVA
GHSDIAPGRKTDPGPYFDWSRFAKQAALPLAMMPYRQAR

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=169, Percent_Identity=57.9881656804734, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=40.7692307692308, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22254; Mature: 22123

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAGRVRDWRPNADGWVPAARHVPSPNFDARPEGMAVDVVVLHNISLPPGQFGSDDIE
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
AFFQNRLDATRHPFFETIRDVRVSAHFLIRRDGEPVQFVACTQRAWHAGQSDYCGRQRCN
HHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECHHHHHCCCCCCCHHHCCC
DFSIGIEIEGTDDQPFTPAQYETVAALVSALRAAYPIQGVAGHSDIAPGRKTDPGPYFDW
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
SRFAKQAALPLAMMPYRQAR
HHHHHHHCCCHHCCCHHCCC
>Mature Secondary Structure 
SDAAGRVRDWRPNADGWVPAARHVPSPNFDARPEGMAVDVVVLHNISLPPGQFGSDDIE
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
AFFQNRLDATRHPFFETIRDVRVSAHFLIRRDGEPVQFVACTQRAWHAGQSDYCGRQRCN
HHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECHHHHHCCCCCCCHHHCCC
DFSIGIEIEGTDDQPFTPAQYETVAALVSALRAAYPIQGVAGHSDIAPGRKTDPGPYFDW
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
SRFAKQAALPLAMMPYRQAR
HHHHHHHCCCHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]