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The map label for this gene is pecT [H]

Identifier: 94312010

GI number: 94312010

Start: 3335105

End: 3336142

Strand: Reverse

Name: pecT [H]

Synonym: Rmet_3079

Alternate gene names: 94312010

Gene position: 3336142-3335105 (Counterclockwise)

Preceding gene: 94312013

Following gene: 335055645

Centisome position: 84.93

GC content: 65.22

Gene sequence:

>1038_bases
ATGACAGGCTCCTTGATTGCGTTGGTTTTCACGGTGCGGTGGATGTTTATCCGCTGCGGTGATGCCATCGTAGGCGCTTC
TGTAATATCGGGAAAGCGGGAATATGCGATAGTATCCATCGGATATACCGAATCATTGGGGCCTGCCATGCGTGGATTCG
AGACCGATCAACTGAGAACCTTCGTGGCCGTGGCGGACAGCGGTAGCTTGTCCGCCGCGGCACCCCGGCTGTTCCTGTCG
CAGTCGTCCGTCAGCGAGCAATTGCGCAAGCTTGAGGAGCGTGCCGGCGTCCCCCTCCTGACTCGAGGGAAGAAGGGGGC
CGCAGTGACGCCGGCCGGCGCGCGGCTGCTCGAGTACGCGCGGCGCATCCTGTCGCTGAACGAACTGGCGATGCAGGAAT
TGCGCGGACAGTCGCTCGATGGCGAGCTACGTCTGGCGGTGACGGACTACTTTCGCCCCGGCGAGATTGCCGGCATGTTG
CGCCGGCTGCGTGACCGCTATCCGTACCTGAAGCTGCACGTGACGGTCATGAAGAGCGCGGCCATCGAGGCTGCCGCCGA
GATGGACGAGTTCGATATCGGCTTGAGTATGCGGTTGATACGTTCGGGGGGCGATGCTGCACCGCACGAGAGGCGCAAGT
CCGGTGTCGTACTGCGGCGGGAGGCGCTGGCGTGGGTCGCGGCACCGGAGATCGCCGAGTCGCCGCAGGCCACGCTGCCG
CTCGTGCTGTTACCGGATACCTGTTCGATGCACCAGTTCGTGGTGCAGTTGCTCAAACGGCGGCGGCGCGCGTTCGATAT
TGGCCATTCGGCATCCGGGGTGGCCGGGCTGCATCTGGCTCTGGCGGCGGGCCTTGGCGTGTCATGTCTGAACGCCTCGG
CCATCGGGCCGGGTGTCGCGCCGCTGGATGCCGCCGCCACGGCGGGCTGGAAATTGCCACCGCTGCCTTCGGCCGAGTTC
TATCTGCTGGGGGCCCGGCGCGGGGAGAGCGAGTTCATTGGCCAGGCGCGTCAGGCGCTGGCCGAGCAGTTCGGCTAG

Upstream 100 bases:

>100_bases
CTTTCGCAGGACCCGCTGGGTCAGTTCGCCGACCGCGGCGACCGCGCGGCGGGTGAGTTCCGTATCGGGATGGCCGGAAA
TCTGCAGGGCGATGTGGGGC

Downstream 100 bases:

>100_bases
GCGAGGAAGACCAGGCCGGGATCAGGTATCGCTCCACTGGCGCAGGAGGTTGTGATAGCAGCCCACTAGCGTGCGGCGCG
CGCGTTCGTCAGCGTTGGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTGSLIALVFTVRWMFIRCGDAIVGASVISGKREYAIVSIGYTESLGPAMRGFETDQLRTFVAVADSGSLSAAAPRLFLS
QSSVSEQLRKLEERAGVPLLTRGKKGAAVTPAGARLLEYARRILSLNELAMQELRGQSLDGELRLAVTDYFRPGEIAGML
RRLRDRYPYLKLHVTVMKSAAIEAAAEMDEFDIGLSMRLIRSGGDAAPHERRKSGVVLRREALAWVAAPEIAESPQATLP
LVLLPDTCSMHQFVVQLLKRRRRAFDIGHSASGVAGLHLALAAGLGVSCLNASAIGPGVAPLDAAATAGWKLPPLPSAEF
YLLGARRGESEFIGQARQALAEQFG

Sequences:

>Translated_345_residues
MTGSLIALVFTVRWMFIRCGDAIVGASVISGKREYAIVSIGYTESLGPAMRGFETDQLRTFVAVADSGSLSAAAPRLFLS
QSSVSEQLRKLEERAGVPLLTRGKKGAAVTPAGARLLEYARRILSLNELAMQELRGQSLDGELRLAVTDYFRPGEIAGML
RRLRDRYPYLKLHVTVMKSAAIEAAAEMDEFDIGLSMRLIRSGGDAAPHERRKSGVVLRREALAWVAAPEIAESPQATLP
LVLLPDTCSMHQFVVQLLKRRRRAFDIGHSASGVAGLHLALAAGLGVSCLNASAIGPGVAPLDAAATAGWKLPPLPSAEF
YLLGARRGESEFIGQARQALAEQFG
>Mature_344_residues
TGSLIALVFTVRWMFIRCGDAIVGASVISGKREYAIVSIGYTESLGPAMRGFETDQLRTFVAVADSGSLSAAAPRLFLSQ
SSVSEQLRKLEERAGVPLLTRGKKGAAVTPAGARLLEYARRILSLNELAMQELRGQSLDGELRLAVTDYFRPGEIAGMLR
RLRDRYPYLKLHVTVMKSAAIEAAAEMDEFDIGLSMRLIRSGGDAAPHERRKSGVVLRREALAWVAAPEIAESPQATLPL
VLLPDTCSMHQFVVQLLKRRRRAFDIGHSASGVAGLHLALAAGLGVSCLNASAIGPGVAPLDAAATAGWKLPPLPSAEFY
LLGARRGESEFIGQARQALAEQFG

Specific function: Regulates pectinase gene expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=289, Percent_Identity=29.757785467128, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1786713, Length=127, Percent_Identity=36.2204724409449, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082132, Length=252, Percent_Identity=26.5873015873016, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=141, Percent_Identity=32.6241134751773, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=146, Percent_Identity=34.2465753424658, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=29.6875, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37053; Mature: 36922

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSLIALVFTVRWMFIRCGDAIVGASVISGKREYAIVSIGYTESLGPAMRGFETDQLRT
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHCCHHHHCCCHHHHEE
FVAVADSGSLSAAAPRLFLSQSSVSEQLRKLEERAGVPLLTRGKKGAAVTPAGARLLEYA
EEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHH
RRILSLNELAMQELRGQSLDGELRLAVTDYFRPGEIAGMLRRLRDRYPYLKLHVTVMKSA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHH
AIEAAAEMDEFDIGLSMRLIRSGGDAAPHERRKSGVVLRREALAWVAAPEIAESPQATLP
HHHHHHHHHHHCCCHHHHEECCCCCCCCHHHHHCCCEEHHHHHHHHCCCHHHCCCCCCCC
LVLLPDTCSMHQFVVQLLKRRRRAFDIGHSASGVAGLHLALAAGLGVSCLNASAIGPGVA
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCC
PLDAAATAGWKLPPLPSAEFYLLGARRGESEFIGQARQALAEQFG
CCHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGSLIALVFTVRWMFIRCGDAIVGASVISGKREYAIVSIGYTESLGPAMRGFETDQLRT
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHCCHHHHCCCHHHHEE
FVAVADSGSLSAAAPRLFLSQSSVSEQLRKLEERAGVPLLTRGKKGAAVTPAGARLLEYA
EEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHH
RRILSLNELAMQELRGQSLDGELRLAVTDYFRPGEIAGMLRRLRDRYPYLKLHVTVMKSA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHH
AIEAAAEMDEFDIGLSMRLIRSGGDAAPHERRKSGVVLRREALAWVAAPEIAESPQATLP
HHHHHHHHHHHCCCHHHHEECCCCCCCCHHHHHCCCEEHHHHHHHHCCCHHHCCCCCCCC
LVLLPDTCSMHQFVVQLLKRRRRAFDIGHSASGVAGLHLALAAGLGVSCLNASAIGPGVA
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCC
PLDAAATAGWKLPPLPSAEFYLLGARRGESEFIGQARQALAEQFG
CCHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]