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The map label for this gene is purB [H]

Identifier: 94311888

GI number: 94311888

Start: 3214134

End: 3215510

Strand: Reverse

Name: purB [H]

Synonym: Rmet_2956

Alternate gene names: 94311888

Gene position: 3215510-3214134 (Counterclockwise)

Preceding gene: 94311890

Following gene: 94311887

Centisome position: 81.86

GC content: 64.42

Gene sequence:

>1377_bases
ATGTCCACGTCTTCCCTTTCGCCGCTTACCGCCCTGTCTCCCATCGATGGCCGCTATGCCGCCAAAGCCGATGCATTGCG
CGAGTGGCTGTCCGAGGCGGCCTTCATGCGCAACCGCGTCAAGGTGGAGGTCAACTGGCTGATCGCGCTGGCCCAGGCCG
GCCTGCCGGACGTGCCGAAGTTCTCGGCCGCATCGGAGGCAAAGCTGCTGTCGCTGGTGGAGAATTTCACCGAGGCCGAC
GCCGCCCGGATCAAGGAAATCGAGGCCGTCACGAACCACGACGTGAAGGCCGTCGAGTACTGGCTCAAGGAGCAGGTCAA
GGGCAACGCGGAGCTCGAGGCCGCCAGCGAGTTCATTCACTTCGCCTGCACGTCGGAAGACATCAACAACACGTCGCACG
GCATGATGCTCAAGGGCGCCCGCGACGGCGTGATCGTGCCGGCGCTCAAGCGCGTGCATGCGCGTCTGGTCGAACTGGCG
AAGCTGAACGCCGCCCAGCCGATGCTGTCGCGTACGCACGGCCAGCCGGCCAGCCCGACCACGCTCGGCAAGGAAATGGC
CAACGTGGCCGCGCGTCTGGCCCGCGCCATCGAGCGTGTGGAACGCGTGGAACTGCTCGGCAAGATGAATGGCGCCGTGG
GTAACTACAACGCGCACCTGTCCGCCTATCCGACGTTCGACTGGGAGTCGTTCTCGAAGCAGGTGATCGAGACGCGCCTG
GGCCTGACGTTCAACCCGTACACGATCCAGATCGAACCGCACGACTACATGGCCGAGCTGTTCGATGCCGTCGCCCGCGC
CAACACGATCCTGCTGGACCTGAACCGCGACGTCTGGGGCTATATCTCGGTGGGCTACTTCAAGCAGAAGACCAAGGCTG
GCGAGATCGGCTCGTCGACCATGCCGCACAAGGTCAACCCGATCGACTTCGAAAACTCGGAGGGCAATGTCGGCCTGGCC
AACGCCGTGCTGCGCCACCTGTCCGAGAAGCTGCCTGTGTCCCGCTGGCAGCGTGACCTGACCGATTCGACCGTGCTGCG
CAACATGGGTGTGGCGTTTGGTTACAGCCTGCTGGCTTACGAGGCCTGCCTGCGTGGCCTGGGCAAGCTGGAAACCAATC
CCGAACGCCTCAACGAAGACCTCGACAACTGCTGGGAAGTGCTGGCGGAGCCGGTGCAGACCGTGATGCGCCGCTTTGGC
GTGCCCAATCCGTACGAGCAATTGAAGGAGCTGACCCGCGGCAAGGGCATCTCGCGCGAGGCGCTCCAGACCTTCGTGCA
GGGTCTGGCGATTCCCGACGATGCCAAGGCGCTGCTGCTGGCGATGACGCCCGCCAGCTACATCGGCCGCGCGGTGGAAC
TGGCGAATCGCATCTGA

Upstream 100 bases:

>100_bases
AATCGCGCCGTGCGGCGCATTGATCGCTTCGCACGTGCCTCGCCCCCGCACCCGCCATGTGCGGAACGCGCCCATACCTC
CCTTACTTCTGGTTGCCAGC

Downstream 100 bases:

>100_bases
CGCTCGAGACGCGTCAGGAAACAAAAGGGCTGGCCTGGTGCCAGCCCTTTTTGTTGTTGCCGGTCGCCAGGGTTGAGGCA
GAAATTACCGCCGTGCGGCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSTSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPKFSAASEAKLLSLVENFTEAD
AARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELA
KLNAAQPMLSRTHGQPASPTTLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRL
GLTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLA
NAVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAYEACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFG
VPNPYEQLKELTRGKGISREALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI

Sequences:

>Translated_458_residues
MSTSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPKFSAASEAKLLSLVENFTEAD
AARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELA
KLNAAQPMLSRTHGQPASPTTLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRL
GLTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLA
NAVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAYEACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFG
VPNPYEQLKELTRGKGISREALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI
>Mature_457_residues
STSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPKFSAASEAKLLSLVENFTEADA
ARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELAK
LNAAQPMLSRTHGQPASPTTLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRLG
LTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLAN
AVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAYEACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFGV
PNPYEQLKELTRGKGISREALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=359, Percent_Identity=26.1838440111421, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4557269, Length=359, Percent_Identity=26.1838440111421, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.1145374449339, Blast_Score=592, Evalue=1e-170,
Organism=Saccharomyces cerevisiae, GI6323391, Length=350, Percent_Identity=25.4285714285714, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50579; Mature: 50448

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPK
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCC
FSAASEAKLLSLVENFTEADAARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
FACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELAKLNAAQPMLSRTHGQPASPT
HEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCH
TLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHC
GLTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSST
CCEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCCCC
MPHKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFGVPNPYEQLKELTRGKGISRE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
ALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI
HHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
STSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPK
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCC
FSAASEAKLLSLVENFTEADAARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
FACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELAKLNAAQPMLSRTHGQPASPT
HEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCH
TLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHC
GLTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSST
CCEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHCCCCCCCCCC
MPHKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFGVPNPYEQLKELTRGKGISRE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
ALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI
HHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]