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The map label for this gene is phcQ [H]

Identifier: 94311881

GI number: 94311881

Start: 3206692

End: 3207690

Strand: Reverse

Name: phcQ [H]

Synonym: Rmet_2949

Alternate gene names: 94311881

Gene position: 3207690-3206692 (Counterclockwise)

Preceding gene: 94311882

Following gene: 94311874

Centisome position: 81.66

GC content: 65.97

Gene sequence:

>999_bases
ATGAGCGAACCGAATACCACGCAGGCACCCCCGGCAATTCTGTTCGTCGATGACGAGGCCACGGCCGTCAAGTATTTCCA
GCGCGCCATTGGCCAGCTTGCCCCGGTGGTGACCGGGGGATCCGTTGAAGAAGGCAAGGCGCTGCTCGACGCCCATGGCG
ACAGCCTGGCCGTGCTGGTGTCCGACCAACGCATGCCGGGCGAATATGGCAACGAGTTGCTGCGTTACGCGCGCGAGCGC
TATCCGCATATCGTACGGATTCTGACCACGGCGTACTCGGAACTCGACCAGACCGTCGAGGCCGTGAACCAGGGCCAGAT
CCATCGCTACATCAAGAAGCCGTGGGACATTACCGCGCTGCGCATGGAGATGAAGCAGGCGCTGGAGTTGTCTGGTCTGC
GCAGGGAGCGTGATCAACTGGTGCGTGAAAAGCTCATGGTGCTGCAGAAGCAGACCGTGGCCACGCGCGTTGGCATGGTC
CACGCGATCTGCGCCAGCCTGATCGGCCCGGGGCGATTCCAGCCGGTGGAGACCTATCTGGCTGGCACGTCGCTGGCGGG
TGCGCGCAATGCCGAGCCCGACTGGCAGCGTCTGGACTATGCGGATCTGGTCGGCGCGGAAAGCCAGCGCTGCGGCGTGT
ACGGCCATGCGGTTGGCACGAAGCTGGCAGAGTTCCGCGCGCGACTGGCTGGTCGTGGCGCTGGTGACGCGACGGCGGTG
CTGGCGGAAGTGCTGGGTGCAGCGGTACGCCGCGACGGCGATGTCGTCACGTGGGCCGCGCCGGACGCGCTCGGTGAGTT
CGTGGCAAGACCCGTGGCCGATGCTGTATCCGACGAGCACGCGGCATGGCTGGCGGCGCTGCTGTGGCTGGAAGAGGCTG
GTGGGGCGCTGCAACTGCAGCGCGAAGGCGACGCGATCACGGCACGCGCGACAACGCGGACTGAGTCGTTCGCCAAGGAC
CGGCTGGCCGTGTGGATCGAGCAGTTGACGGAAGCCTAA

Upstream 100 bases:

>100_bases
CTTTTGGTGGCGGCATCCGGATCGCTTCGGCTTCGGGCGCAGGCACCACCGTAACCCTCGATTTCCCCAATATGAAGAAT
CGTATGAACAGGAGTGATCG

Downstream 100 bases:

>100_bases
GCGTACGTGAGCGTAGTGGCTCCCTCTCCGCAAACGCGGGGGGAGGGGGCCTGAATCGATGCCGCGCGAGCCGGCGCGGT
CATCGAATGTCTTTCTCCAA

Product: response regulaotr, CheY family; belongs to the phcBSRQ operon that regulates phcA

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLVSDQRMPGEYGNELLRYARER
YPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMV
HAICASLIGPGRFQPVETYLAGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAV
LAEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQREGDAITARATTRTESFAKD
RLAVWIEQLTEA

Sequences:

>Translated_332_residues
MSEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLVSDQRMPGEYGNELLRYARER
YPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMV
HAICASLIGPGRFQPVETYLAGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAV
LAEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQREGDAITARATTRTESFAKD
RLAVWIEQLTEA
>Mature_331_residues
SEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLVSDQRMPGEYGNELLRYARERY
PHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMVH
AICASLIGPGRFQPVETYLAGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAVL
AEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQREGDAITARATTRTESFAKDR
LAVWIEQLTEA

Specific function: Probable member of the two-component regulatory system involved in the regulation of the hydrogenase activity. HoxA is probably phosphorylated by a sensory component (which could be hoxX) and then acts in conjunction with sigma-54 as a transcriptional act

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36144; Mature: 36013

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLV
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHCCCCEEEEEE
SDQRMPGEYGNELLRYARERYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITAL
ECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMVHAICASLIGPGRFQPVETYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAV
HCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
LAEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQ
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEE
REGDAITARATTRTESFAKDRLAVWIEQLTEA
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLV
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHCCCCEEEEEE
SDQRMPGEYGNELLRYARERYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITAL
ECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMVHAICASLIGPGRFQPVETYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAV
HCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
LAEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQ
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEE
REGDAITARATTRTESFAKDRLAVWIEQLTEA
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2001989; 12948488 [H]