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The map label for this gene is glcD [H]

Identifier: 94311858

GI number: 94311858

Start: 3181936

End: 3183438

Strand: Direct

Name: glcD [H]

Synonym: Rmet_2926

Alternate gene names: 94311858

Gene position: 3181936-3183438 (Clockwise)

Preceding gene: 94311857

Following gene: 94311859

Centisome position: 81.0

GC content: 67.4

Gene sequence:

>1503_bases
ATGAACGCCCCGCAAGACGCCCAAACTGTCGCCGGTGAATCCGCGGAGACGCGACGCGCCACGCTGCTGGCCGGCCTCGC
CCGCATCCTGCCCGACACGGCCGTGCTGTGGAAGGCCGAGGACACCGTGCCGTACGAATGCGACGGCCTGGCCGCCTATC
GCCAGGTTCCAGACGCCGTGGTGCTGCCCGATACCGAGGAGCAGGTCTGCGCGGTGCTGCGCCTGTGCCACGAGCTCGGC
GTGCCGGTGGTGCCGCGCGGCTCCGGCACCAGCCTGTCTGGCGGCGCGATGCCGATCGCCGGCGGACTGGTTCTGTCGCT
GGCCAAGTTCAAGCGCATCCTGTCGCTCGATCCGTATTCCCGCACGGCGGTGGTTCAGCCCGGCGTGCGCAACCTGGCAA
TCTCCGACGCTGCCGCCCCGCACAACCTCTACTACGCGCCCGATCCATCCTCGCAGATCGCCTGTTCCATCGGCGGCAAC
GTGGCCGAGAACTCGGGTGGCGTGCATTGCCTCAAGTACGGCCTGACGGTCCACAACGTGCTGCGCGTGCGCGCGGTGAC
GATGGAAGGCGAGGTGGTCGTGTTCGGGTCCGAGGCGCCCGACGCGCCGGGCCTCGACCTCCTGGCCGCGATGATCGGCT
CCGAGGGCATGCTGGCCGTGGTCACGGAAGTCACCGTGAGGCTCATTCCCAAACCGCAACTCGCGCAGGTCATCATGGCC
AGCTTCGATGATGTGGAAAAAGGCGGCAATGCCGTGGCCGACGTGATCGCCGCTGGCATTATCCCGGCCGGGCTGGAGAT
GATGGACAAGCCCGCCACGGCCGCCGTGGAGGAGTTCGTCAAGGCCGGCTACGACCTCGACGCCGCCGCGATCCTGCTGT
GCGAGTCGGATGGCACGCCCGAGGAAGTGGCCGAGGAAATCGCCCGCATGAGCGAGGTGCTGCGCGCATCAGGCGCGACG
CGCATCACGGTGTCGCAAAGCGAAGCCGAGCGCCTGCGCTTCTGGAGCGGCCGCAAGAACGCCTTCCCGGCCGCGGGCCG
CATCTCGCCCGACTACTACTGCATGGACGGCACGATTCCCCGCAAGCACATCGGCACGCTGCTCAAGCGCATCGAGCAGA
TGGAGCACAAATACGGGTTGCGCTGCATCAACGTTTTCCATGCGGGCGATGGCAATATGCACCCCCTGATCCTGTTCGAT
GGCGCCGATCACGACGAGTGGCAGCGCGCGGAGCTGTTCGGGTCGGACATCCTGGAGGCCTGCGTGGAACTCGGCGGCAC
CGTGACCGGCGAGCACGGTGTGGGCGTGGAAAAACTCAACTCGATGTGCGTGCAGTTCTCCCCGTCCGAGCGCGACGCGT
TCTTCGGGGTCAAGGCCGCATTCGATCCCGCCCGGCTGCTGAACCCGGACAAGGCAATCCCCACGCTTGCGCGCTGCGCC
GAATACGGCAAGATGCACGTCAAGCGCGGCCTGCTGCCGCATCCGGACCTGCCGCGTTTCTGA

Upstream 100 bases:

>100_bases
CGGCGGGGTGCCTGCGCTATAGTCTGCCGTCCCGGCCCCGGTACGAGCACGCATCCACGCGGGAATCGTGCGCGTCGGCC
GGTCCGTCACAGGAGTTGTC

Downstream 100 bases:

>100_bases
CACTATCCAGACGGGAAGGCGCGAGAGTCCGCAACCCTTCTCCGCCTTGCACATGTCACGATCCACACGATCCGATCAAA
ACGCGCGCATTCGTCAACGG

Product: glycolate oxidase subunit, FAD-linked

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNAPQDAQTVAGESAETRRATLLAGLARILPDTAVLWKAEDTVPYECDGLAAYRQVPDAVVLPDTEEQVCAVLRLCHELG
VPVVPRGSGTSLSGGAMPIAGGLVLSLAKFKRILSLDPYSRTAVVQPGVRNLAISDAAAPHNLYYAPDPSSQIACSIGGN
VAENSGGVHCLKYGLTVHNVLRVRAVTMEGEVVVFGSEAPDAPGLDLLAAMIGSEGMLAVVTEVTVRLIPKPQLAQVIMA
SFDDVEKGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVKAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGAT
RITVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIEQMEHKYGLRCINVFHAGDGNMHPLILFD
GADHDEWQRAELFGSDILEACVELGGTVTGEHGVGVEKLNSMCVQFSPSERDAFFGVKAAFDPARLLNPDKAIPTLARCA
EYGKMHVKRGLLPHPDLPRF

Sequences:

>Translated_500_residues
MNAPQDAQTVAGESAETRRATLLAGLARILPDTAVLWKAEDTVPYECDGLAAYRQVPDAVVLPDTEEQVCAVLRLCHELG
VPVVPRGSGTSLSGGAMPIAGGLVLSLAKFKRILSLDPYSRTAVVQPGVRNLAISDAAAPHNLYYAPDPSSQIACSIGGN
VAENSGGVHCLKYGLTVHNVLRVRAVTMEGEVVVFGSEAPDAPGLDLLAAMIGSEGMLAVVTEVTVRLIPKPQLAQVIMA
SFDDVEKGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVKAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGAT
RITVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIEQMEHKYGLRCINVFHAGDGNMHPLILFD
GADHDEWQRAELFGSDILEACVELGGTVTGEHGVGVEKLNSMCVQFSPSERDAFFGVKAAFDPARLLNPDKAIPTLARCA
EYGKMHVKRGLLPHPDLPRF
>Mature_500_residues
MNAPQDAQTVAGESAETRRATLLAGLARILPDTAVLWKAEDTVPYECDGLAAYRQVPDAVVLPDTEEQVCAVLRLCHELG
VPVVPRGSGTSLSGGAMPIAGGLVLSLAKFKRILSLDPYSRTAVVQPGVRNLAISDAAAPHNLYYAPDPSSQIACSIGGN
VAENSGGVHCLKYGLTVHNVLRVRAVTMEGEVVVFGSEAPDAPGLDLLAAMIGSEGMLAVVTEVTVRLIPKPQLAQVIMA
SFDDVEKGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVKAGYDLDAAAILLCESDGTPEEVAEEIARMSEVLRASGAT
RITVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKHIGTLLKRIEQMEHKYGLRCINVFHAGDGNMHPLILFD
GADHDEWQRAELFGSDILEACVELGGTVTGEHGVGVEKLNSMCVQFSPSERDAFFGVKAAFDPARLLNPDKAIPTLARCA
EYGKMHVKRGLLPHPDLPRF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=423, Percent_Identity=29.3144208037825, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI37595754, Length=446, Percent_Identity=28.47533632287, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI119964728, Length=470, Percent_Identity=27.2340425531915, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4501993, Length=223, Percent_Identity=25.5605381165919, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789351, Length=493, Percent_Identity=60.0405679513185, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=22.9674796747967, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787977, Length=511, Percent_Identity=21.7221135029354, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71992373, Length=428, Percent_Identity=27.3364485981308, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=428, Percent_Identity=26.6355140186916, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=387, Percent_Identity=24.5478036175711, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17534361, Length=447, Percent_Identity=26.3982102908277, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=285, Percent_Identity=22.4561403508772, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320023, Length=475, Percent_Identity=27.7894736842105, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320027, Length=434, Percent_Identity=25.5760368663594, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320764, Length=480, Percent_Identity=24.7916666666667, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18921117, Length=464, Percent_Identity=27.5862068965517, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24639277, Length=464, Percent_Identity=27.5862068965517, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24639275, Length=464, Percent_Identity=27.5862068965517, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=29.2792792792793, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53304; Mature: 53304

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQDAQTVAGESAETRRATLLAGLARILPDTAVLWKAEDTVPYECDGLAAYRQVPDAV
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHCCCEE
VLPDTEEQVCAVLRLCHELGVPVVPRGSGTSLSGGAMPIAGGLVLSLAKFKRILSLDPYS
ECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RTAVVQPGVRNLAISDAAAPHNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNV
CCEEECCCCHHEEECCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHH
LRVRAVTMEGEVVVFGSEAPDAPGLDLLAAMIGSEGMLAVVTEVTVRLIPKPQLAQVIMA
HEEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCEEHEEHHHHHCCCCHHHHHHHHH
SFDDVEKGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVKAGYDLDAAAILLCESDGTP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCH
EEVAEEIARMSEVLRASGATRITVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
RKHIGTLLKRIEQMEHKYGLRCINVFHAGDGNMHPLILFDGADHDEWQRAELFGSDILEA
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
CVELGGTVTGEHGVGVEKLNSMCVQFSPSERDAFFGVKAAFDPARLLNPDKAIPTLARCA
HHHHCCEEECCCCCCHHHHHCHHEEECCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHH
EYGKMHVKRGLLPHPDLPRF
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNAPQDAQTVAGESAETRRATLLAGLARILPDTAVLWKAEDTVPYECDGLAAYRQVPDAV
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHCCCEE
VLPDTEEQVCAVLRLCHELGVPVVPRGSGTSLSGGAMPIAGGLVLSLAKFKRILSLDPYS
ECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
RTAVVQPGVRNLAISDAAAPHNLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNV
CCEEECCCCHHEEECCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCHHHHH
LRVRAVTMEGEVVVFGSEAPDAPGLDLLAAMIGSEGMLAVVTEVTVRLIPKPQLAQVIMA
HEEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCEEHEEHHHHHCCCCHHHHHHHHH
SFDDVEKGGNAVADVIAAGIIPAGLEMMDKPATAAVEEFVKAGYDLDAAAILLCESDGTP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCH
EEVAEEIARMSEVLRASGATRITVSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
RKHIGTLLKRIEQMEHKYGLRCINVFHAGDGNMHPLILFDGADHDEWQRAELFGSDILEA
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
CVELGGTVTGEHGVGVEKLNSMCVQFSPSERDAFFGVKAAFDPARLLNPDKAIPTLARCA
HHHHCCEEECCCCCCHHHHHCHHEEECCCCCCCEEEECCCCCHHHHCCCCHHHHHHHHHH
EYGKMHVKRGLLPHPDLPRF
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]