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The map label for this gene is gcvA [H]

Identifier: 94311857

GI number: 94311857

Start: 3180832

End: 3181722

Strand: Direct

Name: gcvA [H]

Synonym: Rmet_2925

Alternate gene names: 94311857

Gene position: 3180832-3181722 (Clockwise)

Preceding gene: 94311856

Following gene: 94311858

Centisome position: 80.98

GC content: 66.55

Gene sequence:

>891_bases
ATGGCATCGGTTTTCCATCGCGTCCCACCCCTGCACCTGCTGATCGCGTTCGAAGCGGCTGCCCGGCTGGGCAGCTTCGC
GCGCGCTGCCGAGGAGCTCTCGGTGACGCCGAGCGCGGTCTCGCACAGGATCAAGAACCTTGAGGAACTCTGGGGCGACG
ACCTGTTCGTGCGCTCGAACGCCGCGCTGCGGCTGACCGCGGCCGGCACGCGCTACCAGCGCAACGTGCAGGATGCGCTG
AAGTCGCTCAACGAACTGGCGCGCCCCGAGTACAACAAGCTGCGCACACGGCTGCGCGTGGCGATTCCGCCGACATTCGG
TCGCCAGCACCTGGTGCCGCGCCTGCCCGAGTTCACCGCGCTCTATCCGCATATCGATCTCGAACTGCACCTGGCCGTGC
CGTTCCTCGACGTCAAGGCCGAGGACACCGATATCGAGATCCGCTACGGCACCGGCCGCTACCCCGACCTGAAGACCACG
AAACTGCTGGTCGAGCCCGTGTTTCCGGCGTGCGGGCGCGAGTACTACGAGCGCGTGAACGGCTTTGCGGTCACCAAACC
CGAGGATCTGCACAATCTGACGCTGCTGCGCAGCCCGCTGGAACCGTGGCGGCCGTGGTTCGAAACCGCCTGCCTGGACT
GGCCGGAGCCGCAGACTGGCGCGCAGTTCAACGATATCGGCCTGATGCTGGAGGCGATCGTCTCGAACCAGGGCGTGGGA
CTGGTGCGCCAGCGCATGGCGCGGCAGTGGCTGGCGGCGGGCCAGATGGTGAAGCTGCTCGATATCGAGTCGGTATCGCC
ACACGGCTACTACATCGTCGAACGCGAGCAGGCGCCGCTGATGCCCGAGGCGCGCTACTTCATCGACTGGCTGCTCGGGC
TGGACTGGTAG

Upstream 100 bases:

>100_bases
GATGCGCTCGACCTGATGCGCGCGATCAAGGATGCGCTCGACCCGAACCACATCCTGAATCCGGGCAAGATCTTCAGCGC
CACGCGCTGAGCACGCGGCC

Downstream 100 bases:

>100_bases
CGGGCAAGCCACTACGCTGGAAGCGGCTTCGTCCGCGACCTTGGGCATTTGCACCCGGCATGAACCTTTTTGATCGGGTC
GTCGTGCCGAATTCACGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MASVFHRVPPLHLLIAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGDDLFVRSNAALRLTAAGTRYQRNVQDAL
KSLNELARPEYNKLRTRLRVAIPPTFGRQHLVPRLPEFTALYPHIDLELHLAVPFLDVKAEDTDIEIRYGTGRYPDLKTT
KLLVEPVFPACGREYYERVNGFAVTKPEDLHNLTLLRSPLEPWRPWFETACLDWPEPQTGAQFNDIGLMLEAIVSNQGVG
LVRQRMARQWLAAGQMVKLLDIESVSPHGYYIVEREQAPLMPEARYFIDWLLGLDW

Sequences:

>Translated_296_residues
MASVFHRVPPLHLLIAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGDDLFVRSNAALRLTAAGTRYQRNVQDAL
KSLNELARPEYNKLRTRLRVAIPPTFGRQHLVPRLPEFTALYPHIDLELHLAVPFLDVKAEDTDIEIRYGTGRYPDLKTT
KLLVEPVFPACGREYYERVNGFAVTKPEDLHNLTLLRSPLEPWRPWFETACLDWPEPQTGAQFNDIGLMLEAIVSNQGVG
LVRQRMARQWLAAGQMVKLLDIESVSPHGYYIVEREQAPLMPEARYFIDWLLGLDW
>Mature_295_residues
ASVFHRVPPLHLLIAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGDDLFVRSNAALRLTAAGTRYQRNVQDALK
SLNELARPEYNKLRTRLRVAIPPTFGRQHLVPRLPEFTALYPHIDLELHLAVPFLDVKAEDTDIEIRYGTGRYPDLKTTK
LLVEPVFPACGREYYERVNGFAVTKPEDLHNLTLLRSPLEPWRPWFETACLDWPEPQTGAQFNDIGLMLEAIVSNQGVGL
VRQRMARQWLAAGQMVKLLDIESVSPHGYYIVEREQAPLMPEARYFIDWLLGLDW

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=34.6020761245675, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=34.2560553633218, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788706, Length=286, Percent_Identity=32.1678321678322, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=28.7625418060201, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI157672245, Length=233, Percent_Identity=31.7596566523605, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786401, Length=268, Percent_Identity=27.2388059701493, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=24.8251748251748, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081978, Length=162, Percent_Identity=32.0987654320988, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=243, Percent_Identity=27.5720164609054, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33792; Mature: 33661

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVFHRVPPLHLLIAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGDDLFVRSN
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
AALRLTAAGTRYQRNVQDALKSLNELARPEYNKLRTRLRVAIPPTFGRQHLVPRLPEFTA
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCCCCCCCCCHHE
LYPHIDLELHLAVPFLDVKAEDTDIEIRYGTGRYPDLKTTKLLVEPVFPACGREYYERVN
ECCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
GFAVTKPEDLHNLTLLRSPLEPWRPWFETACLDWPEPQTGAQFNDIGLMLEAIVSNQGVG
CEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
LVRQRMARQWLAAGQMVKLLDIESVSPHGYYIVEREQAPLMPEARYFIDWLLGLDW
HHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASVFHRVPPLHLLIAFEAAARLGSFARAAEELSVTPSAVSHRIKNLEELWGDDLFVRSN
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECC
AALRLTAAGTRYQRNVQDALKSLNELARPEYNKLRTRLRVAIPPTFGRQHLVPRLPEFTA
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCCCCCCCCCHHE
LYPHIDLELHLAVPFLDVKAEDTDIEIRYGTGRYPDLKTTKLLVEPVFPACGREYYERVN
ECCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHC
GFAVTKPEDLHNLTLLRSPLEPWRPWFETACLDWPEPQTGAQFNDIGLMLEAIVSNQGVG
CEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHH
LVRQRMARQWLAAGQMVKLLDIESVSPHGYYIVEREQAPLMPEARYFIDWLLGLDW
HHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]