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The map label for this gene is ispH [H]

Identifier: 94311800

GI number: 94311800

Start: 3118860

End: 3119852

Strand: Reverse

Name: ispH [H]

Synonym: Rmet_2868

Alternate gene names: 94311800

Gene position: 3119852-3118860 (Counterclockwise)

Preceding gene: 94311801

Following gene: 94311799

Centisome position: 79.42

GC content: 65.06

Gene sequence:

>993_bases
ATGTCTGCCGTTGTAACGGAACGCAGCATGACCGAAACCACTGTCCCTGCCGATGCCGAGATCCTGATGGCGCAGCCGCG
CGGCTTCTGCGCGGGCGTCGATCGTGCGATCGAAATCGTCGAACGCGCGCTGTCGCGGTTCGGCGCTCCTATCTATGTGC
GCCACGAGATCGTCCACAACGCCTACGTGGTGGCCGACCTGCGCAAGAAAGGCGCGATCTTCGTCGACGAGCTCGACGAA
GTGCCGCAGGGCTCGACGGTTATCTTCAGCGCCCATGGCGTATCGAAGGAAGTGCGCGCCGACGCCACAGCGCGAGGTCT
GACCGTTTTCGATGCCACCTGCCCGCTGGTGACCAAGGTGCACGTGGAAGTCGGCAAGATGCGCGGCGAGGGTTGCGAGA
TCATCATGATCGGCCACCGGGGCCACCCCGAAGTGGAAGGCACGATGGGCCAATCCGAACACGGGATGCTGCTGGTGGAA
TCGGTGGACGACGTGAATCGTCTGGTCGTTGCCGATCCGCAACAACTGGCCTACGTCACGCAGACCACGCTGTCGGTGGA
CGAAACCGCCGAGATCGTGGCCGCGCTCAAGGTCCGCTTCCCGCAGATCCGCGAGCCGAAGAAGCAGGACATCTGCTACG
CGACGCAGAACCGCCAGGACGCCGTGAAGTTCATGGTGCCGCAGGTGGAAGTGGTGATCGTGGTGGGCAGCCCGAACAGC
TCGAACTCGAACCGCCTGCGCGAACTGGCCGACCGTCTGGGCGTGCCGGCGTACATGGTGGACGATCCGGAACAGCTCAA
GCCGGAATGGGTGACTGGCAAGCGCCGCATTGGCCTGACAGCCGGCGCCTCGGCGCCCGAGGCGCTCGCCCAGGCAATCG
TCGAGCGTCTGCGGACCTTCGGCGTCAAGAGCGTGCGTGCGCTCGAGGGCGTGGAAGAGAACATGTCGTTCCCGCTGCCG
CGCGGCCTGGCCAGCGTGTCGGTCCAGTCCTGA

Upstream 100 bases:

>100_bases
TGCTCAAGGAGTACGGCGAGACCGCCGCGCTTTTCGATTTCAACCACCCGCTGGCCGGACAGGCAATTCTGTTCGACGTC
CAGCTGATCGGAATTCTCTG

Downstream 100 bases:

>100_bases
TCCTGAAGCCCTGAGTTCCCGAGGCCGGGCGCCCTGCCTGCCTCGTCCCTGCCGGACACCTCACGCGTACGCGTGACCGT
GTCCGCGCCGAAATCCCTCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSAVVTERSMTETTVPADAEILMAQPRGFCAGVDRAIEIVERALSRFGAPIYVRHEIVHNAYVVADLRKKGAIFVDELDE
VPQGSTVIFSAHGVSKEVRADATARGLTVFDATCPLVTKVHVEVGKMRGEGCEIIMIGHRGHPEVEGTMGQSEHGMLLVE
SVDDVNRLVVADPQQLAYVTQTTLSVDETAEIVAALKVRFPQIREPKKQDICYATQNRQDAVKFMVPQVEVVIVVGSPNS
SNSNRLRELADRLGVPAYMVDDPEQLKPEWVTGKRRIGLTAGASAPEALAQAIVERLRTFGVKSVRALEGVEENMSFPLP
RGLASVSVQS

Sequences:

>Translated_330_residues
MSAVVTERSMTETTVPADAEILMAQPRGFCAGVDRAIEIVERALSRFGAPIYVRHEIVHNAYVVADLRKKGAIFVDELDE
VPQGSTVIFSAHGVSKEVRADATARGLTVFDATCPLVTKVHVEVGKMRGEGCEIIMIGHRGHPEVEGTMGQSEHGMLLVE
SVDDVNRLVVADPQQLAYVTQTTLSVDETAEIVAALKVRFPQIREPKKQDICYATQNRQDAVKFMVPQVEVVIVVGSPNS
SNSNRLRELADRLGVPAYMVDDPEQLKPEWVTGKRRIGLTAGASAPEALAQAIVERLRTFGVKSVRALEGVEENMSFPLP
RGLASVSVQS
>Mature_329_residues
SAVVTERSMTETTVPADAEILMAQPRGFCAGVDRAIEIVERALSRFGAPIYVRHEIVHNAYVVADLRKKGAIFVDELDEV
PQGSTVIFSAHGVSKEVRADATARGLTVFDATCPLVTKVHVEVGKMRGEGCEIIMIGHRGHPEVEGTMGQSEHGMLLVES
VDDVNRLVVADPQQLAYVTQTTLSVDETAEIVAALKVRFPQIREPKKQDICYATQNRQDAVKFMVPQVEVVIVVGSPNSS
NSNRLRELADRLGVPAYMVDDPEQLKPEWVTGKRRIGLTAGASAPEALAQAIVERLRTFGVKSVRALEGVEENMSFPLPR
GLASVSVQS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=307, Percent_Identity=59.2833876221498, Blast_Score=373, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35910; Mature: 35778

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVVTERSMTETTVPADAEILMAQPRGFCAGVDRAIEIVERALSRFGAPIYVRHEIVHN
CCCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHC
AYVVADLRKKGAIFVDELDEVPQGSTVIFSAHGVSKEVRADATARGLTVFDATCPLVTKV
EEEEEEHHHCCCEEEEHHHCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEECCCCEEEEE
HVEVGKMRGEGCEIIMIGHRGHPEVEGTMGQSEHGMLLVESVDDVNRLVVADPQQLAYVT
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECHHHHEEEH
QTTLSVDETAEIVAALKVRFPQIREPKKQDICYATQNRQDAVKFMVPQVEVVIVVGSPNS
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHCCCCEEEEEEEECCCC
SNSNRLRELADRLGVPAYMVDDPEQLKPEWVTGKRRIGLTAGASAPEALAQAIVERLRTF
CCHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
GVKSVRALEGVEENMSFPLPRGLASVSVQS
CHHHHHHHHCHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
SAVVTERSMTETTVPADAEILMAQPRGFCAGVDRAIEIVERALSRFGAPIYVRHEIVHN
CCEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHC
AYVVADLRKKGAIFVDELDEVPQGSTVIFSAHGVSKEVRADATARGLTVFDATCPLVTKV
EEEEEEHHHCCCEEEEHHHCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEECCCCEEEEE
HVEVGKMRGEGCEIIMIGHRGHPEVEGTMGQSEHGMLLVESVDDVNRLVVADPQQLAYVT
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECHHHHEEEH
QTTLSVDETAEIVAALKVRFPQIREPKKQDICYATQNRQDAVKFMVPQVEVVIVVGSPNS
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHCCCCEEEEEEEECCCC
SNSNRLRELADRLGVPAYMVDDPEQLKPEWVTGKRRIGLTAGASAPEALAQAIVERLRTF
CCHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
GVKSVRALEGVEENMSFPLPRGLASVSVQS
CHHHHHHHHCHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]