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The map label for this gene is livK2 [H]

Identifier: 94311799

GI number: 94311799

Start: 3116980

End: 3118185

Strand: Reverse

Name: livK2 [H]

Synonym: Rmet_2867

Alternate gene names: 94311799

Gene position: 3118185-3116980 (Counterclockwise)

Preceding gene: 94311800

Following gene: 94311798

Centisome position: 79.38

GC content: 61.94

Gene sequence:

>1206_bases
ATGCAATTCACGTTTGCCAAGATTCTGCCGATCGCGGCCGCTGTGGCGCTGGTCGCTGCATGTGGCAAGAAAGAAGAAAA
GCCGGTTGAATCGGCCGCACCGGCACCCGCTGCCACGGCGGCAGCCGACACGAGTGGCGGCGAGACCATCGTCAAGATCG
GTCACGCGGCGCCCCTGACCGGCGGCATTGCCCACCTTGGCAAGGACAACGAGAACGGCGCCCGCCTGGCCGTGGAAGAG
GTCAACAAGGATGGCCTGACCATCAACGGCAAGAAGATCAAGCTGGAACTGGTTGGCGAAGACGACGCGGCCGATCCGAA
GACCGGTACCGCCGTGGCCCAGAAGCTGGTGGACGCCAAGGTCGTGGCCGTGGTGGGCCACCTGAACTCGGGCGTGTCGA
TCCCGGCTTCGAAGATCTACAGCGATGCCGGCATCGTCCAGATCTCGCCGTCGTCGACCAACCCCGACTACACCAAGCAG
GGCTTCAAGACCACCTATCGCGTGGTGGCGACGGATGCCCAGCAAGGTCCGGCACTGGCCAACTACGCTGCCAAGACCCT
GCACGCCAAGACCGTGGCCATCGTTGACGATGCCACCGCCTACGGCAAGGGCCTGGCCGATGAGTTCGAGAAGACCTCGA
AGGCCGATGGTGTGAACGTGGTGGCACGTGAAGCCACCAACGACAAGGCCACCGACTTCAAGGCCATTCTGACCAAGATC
AAGGGCAAGAAGCCCGACGTGATCATGTACGGTGGCATGGACGCCACCGGCGGTCCGTTCGCCAAGCAGGCCAAGGAACT
GGGCATCACGGCGAAGATCGTTGGCGGCGATGGTGTTTGTACCGACAAGGTGGCCGAACTGGCTGGCGATGCGGTGGACA
ACCTCATCTGCTCGGAAGCCGGTCTGGCACTGTCCAAGATGGAGAAGGGCGCCGACTTCGAGAAGCGCTACCAGGCACGT
TTCAACTCGCCGGTGCAGATCTACGCGCCGTTCACCTATGACGCCGTCATGGTGATCGTCGACGCGATGAAGCGCGCCAA
TTCGACCGACGCGGCGGCCATCCTGGCCGAGATGCCGAAGACGAACTACAAGGGCGTGATCGGCAACATCGCCTTCGACG
AGAAGGGCGATATGAAGGAGGGCACCATCACCCTGTACGACTACAAGGACAAGAAGAAGTCCGTCCTCGACGTCGTGAAG
ATGTAA

Upstream 100 bases:

>100_bases
ATTCGCGCGTTTCAAATTGAAACTAAACAAAGGCGGTTCGGGGAATACGTCCGGGAGGCGTGTTTTATTCCCTGTTTTTG
TGAATCCTAGGAGATCACTC

Downstream 100 bases:

>100_bases
TCAGGGCTTACCAGCCCGGACGAACGGCACCGTGAGCACTTTTACGGTGCCGTTCTTTTTAGTCTCGCCAACGTGTTGAC
AGCATCCTTACCAAGGGTGG

Product: HAAT family ABC transporter periplasmic protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLTGGIAHLGKDNENGARLAVEE
VNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQ
GFKTTYRVVATDAQQGPALANYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKI
KGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEAGLALSKMEKGADFEKRYQAR
FNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVK
M

Sequences:

>Translated_401_residues
MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLTGGIAHLGKDNENGARLAVEE
VNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQ
GFKTTYRVVATDAQQGPALANYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKI
KGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEAGLALSKMEKGADFEKRYQAR
FNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVK
M
>Mature_401_residues
MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLTGGIAHLGKDNENGARLAVEE
VNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQ
GFKTTYRVVATDAQQGPALANYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKI
KGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEAGLALSKMEKGADFEKRYQAR
FNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVK
M

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=345, Percent_Identity=31.5942028985507, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI48994941, Length=338, Percent_Identity=29.585798816568, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41836; Mature: 41836

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHCCCCCCCCEEEEECCCCCCC
GGIAHLGKDNENGARLAVEEVNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAK
CCHHHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQGFKTTYRVVATDAQQGPALA
HEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCHHH
NYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKI
HHHHHHHHHEEEEEEECCHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
KGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEA
CCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GLALSKMEKGADFEKRYQARFNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPK
CCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVKM
CCCCEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHCCCCCCCCEEEEECCCCCCC
GGIAHLGKDNENGARLAVEEVNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAK
CCHHHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQGFKTTYRVVATDAQQGPALA
HEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCHHH
NYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKI
HHHHHHHHHEEEEEEECCHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
KGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEA
CCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GLALSKMEKGADFEKRYQARFNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPK
CCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVKM
CCCCEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]