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The map label for this gene is paaF [C]

Identifier: 94311727

GI number: 94311727

Start: 3042145

End: 3042927

Strand: Reverse

Name: paaF [C]

Synonym: Rmet_2795

Alternate gene names: 94311727

Gene position: 3042927-3042145 (Counterclockwise)

Preceding gene: 94311728

Following gene: 94311726

Centisome position: 77.47

GC content: 68.07

Gene sequence:

>783_bases
ATGAGCGACACCGTCACGTTCTCGCGCGAAGGGTCGACGGCCGTCGTCACGCTGTCGAATCCGGGCAAGCTCAATGCAAT
CTCGGTCGACATGTGGCAGGCGCTGGCCGCCGGATTCGCCACGCTGGCGGCGGACATGTCGCTGCGCTGCGTGCTTGTGC
GCGGTGCCGACGGCAACTTCGCCGCCGGCGCCGACATTGCGGAGTTTCCGACTGTGCGCGGAGACGAGGCGGCTGTCCGC
CGCTACCACACGGAAATCATCGCACCGGCGCTGCGGGCGATTTCAGAATGCCCGCATCCGACGCTGGCCGCCATCGAGGG
CGTTTGCGTTGGCGGTGGCCTCGAGATTGCCTGCAATTGCGACCTGCGCGTGGCCGCTGCGGGCAGCCGCTTCGGCGTGC
CGATCAACCGGCTCGGCTTTCCGATGGCGCCCGGCGAGTTGCGCGGCCTGCTTGCGCTGGTGGGGCGGGCGGTGACGCTC
GAGATCCTGCTCGAAGGCCGTGTGTTCGATGCACTGGAGGCTGAGCGCAAGGGGCTGCTCACGCGAGTGGTGCCTGCGGC
GGCCATGCACGACGAAGTGACGGCAACCGTGCAGCGGCTGAGCGCCGGCGCGCCGCTTGCGGCCCGCATCAACAAGACGA
CGATTCGTCGCCTGTCGCCAGATCCCACGCTGCTGACCGACGCCGAATTCGACGCCCATTTCCGTTACGCCACCAGTCGC
GACCATGCCGAGGGCGTGGCCGCGTTTCTGGCGCACCGGCCTCCGGATTTCACTGGAGAGTAA

Upstream 100 bases:

>100_bases
ACATCCCGCAGTACACGCCGGAGCAGTTCGCGCAATTCCAGCACGCCGAGATCAAGCGCTGGGCAGACGTGGTGCAGCGC
TCGGGCGCGAAGATCGACTG

Downstream 100 bases:

>100_bases
CCGGGTGGCGGCCGGGCTATCATTACCGGCCGCACCGTATCGCCAAGGCTCCCCGGGTCCGCTTTTCCCAGCATTCCGTA
CCTGTCCCGTACTTGTTTCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNFAAGADIAEFPTVRGDEAAVR
RYHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNCDLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTL
EILLEGRVFDALEAERKGLLTRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSR
DHAEGVAAFLAHRPPDFTGE

Sequences:

>Translated_260_residues
MSDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNFAAGADIAEFPTVRGDEAAVR
RYHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNCDLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTL
EILLEGRVFDALEAERKGLLTRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSR
DHAEGVAAFLAHRPPDFTGE
>Mature_259_residues
SDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNFAAGADIAEFPTVRGDEAAVRR
YHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNCDLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTLE
ILLEGRVFDALEAERKGLLTRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSRD
HAEGVAAFLAHRPPDFTGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=28.8, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=22.8346456692913, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI70995211, Length=210, Percent_Identity=29.5238095238095, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI68989263, Length=199, Percent_Identity=32.6633165829146, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4502327, Length=243, Percent_Identity=30.0411522633745, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=188, Percent_Identity=30.3191489361702, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=29.1187739463602, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=29.9242424242424, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=30.4029304029304, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=26, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=27.906976744186, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=31.1224489795918, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=27.4611398963731, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=29.1187739463602, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=28.968253968254, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=29.0476190476191, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=28.3185840707965, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=263, Percent_Identity=26.9961977186312, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=230, Percent_Identity=27.8260869565217, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=263, Percent_Identity=25.4752851711027, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=26.1682242990654, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=29.296875, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=29.296875, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=29.0076335877863, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19920382, Length=211, Percent_Identity=25.1184834123223, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.1379310344828, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357171, Length=184, Percent_Identity=29.3478260869565, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24650670, Length=183, Percent_Identity=32.2404371584699, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27386; Mature: 27254

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNF
CCCCEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
AAGADIAEFPTVRGDEAAVRRYHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCEEEEEEC
DLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTLEILLEGRVFDALEAERKGLL
CEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
TRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSR
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHCCCCCCCEEECCCCCCEEEECCCC
DHAEGVAAFLAHRPPDFTGE
CHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNF
CCCEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
AAGADIAEFPTVRGDEAAVRRYHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCEEEEEEC
DLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTLEILLEGRVFDALEAERKGLL
CEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHH
TRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSR
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHCCCCCCCEEECCCCCCEEEECCCC
DHAEGVAAFLAHRPPDFTGE
CHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA