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The map label for this gene is btuB [H]

Identifier: 94311721

GI number: 94311721

Start: 3035218

End: 3037314

Strand: Reverse

Name: btuB [H]

Synonym: Rmet_2789

Alternate gene names: 94311721

Gene position: 3037314-3035218 (Counterclockwise)

Preceding gene: 94311726

Following gene: 94311720

Centisome position: 77.32

GC content: 64.14

Gene sequence:

>2097_bases
ATGCCGCGCCGAAGGTGGCATACCCCATCGCCATATGGCCCGCGGGGGAACGGGCCTGGCCGTTCTCTTCCATCCCTGCT
CATGCGTTCCACGCTATCCAGGTCGACCGCCGCGCTGAAGCCGTCGGTTCGCCCCTCCATGCCCGCCATCCTGGCGGCCA
TCGCCCTTTTTCCGTCCGCATCGGTCTTCGCGCAGGACGCGACGCCAGATGAGTCGTCGACCACCACGCCGGCCAGCGCA
TCGGCCAATGGTCCCGCTCCCGCTTCCGCACCGGCAGCCGCACCGGCCGCCGCGCAGGCGGCCAGCGGGCAGACGCTGGC
GCCCGTAGTCGTCACCGCCTCCCGAACCGCGCAGTCGGTGACCGAGGCCCTGCCGCACACCACGGTGGTCACCCAGGAAG
ACATCGTCAACTCACAGGCTCCGGACCTGCGCTCGCTACTGCGCAACCAGGCGGGCGTGGAGTTCTCGACCAACGGCGGC
ATGGGTACCAATACCTCGCTGTTCATGCGCGGCGCCAATTCGAACCAGGTGCTGATCATGATCGACGGCGTGCGGATTTC
CGGCGTCAGCAACGGTAATGCGCAGATCGCCAATATCTTGCCCGACCAGATCGACCATATCGAAGTGGTGCGCGGCAACG
TCTCGGCGCTCTATGGCTCCGACGCCGTTGGTGGCGTGGTGCAGATCTTCACCAAGAGTGGCGCGGGCCAGGCGCCTGCC
GCCAACGCGCAGGTGGAGTACGGCAGCAACAATACCCGCCAGGGCACGGTGGGTTACGGTGGCCAGATCGGTGCCACCTC
GTTCAACGTCACCGGCTCGATGTACAAGACCGATGGCTTCTCGGCCATCAATGCGGCGCAGTGGAACGCCGACAATCCGA
AGAGCGCCGCGCGCGGTATCGGCCCGAATCCGGCCAACAACCCGTACGAGAACAAGAGCATCTCGGGCCAGATCAAGCAC
CAGTTCACGCAGGACTGGGACGCTGGCGTCGCGACCTACTACTCGAACAGCCAGCTTTCCTACGACAACGCCTACGGCAA
GCCGACCGACGACTACCGCATGGATAGCAAGCTCTACACGCTGTCCGCGTTCGTCAACGGCAAGGTGCTATCTGACTGGA
CCACGCATTTCAAGGTTGCGCAAAGCGAAGACCGCAGCGAAGGCACGATCAACGGCCTGTTCGACAGCCTGTTCAGCACG
CGCACCCGCCAGTTCAACTGGCAGAACGAGTACGCGCTGACACCCGACCATACGCTGATGTTTGGCACGGACTGGCTGCA
GCAGGAGCTGGCTTCGAGCTCGTACAACGCGCCGTCGCGCAATGTGTTTTCGGTCTATGGCGGCTACGAGGGGCGTATCG
GCAAGCACCAGATCCAGTTGAACGGCCGTAACGACCACTATTCCGATTTCGGCGACCAGGGCAGCTTCTTCGCCGGCTAC
GGCTACTACCTGACCAGCGCGGTCAAGTTGCTTGCCAACGTCAGCAATGCGTTCCGCGCGCCGACGTTCAACGAGCTCTA
CTACCCGTACTACGGCCAGCCGAACCTGCAGCCCGAGCGCGCCAAGTCGGCGGAGGTGGGGGTGCAGGTGGAGACGGCGT
CGACCGGCCTGCTGCGCGTGACCGCGTTCGAGACGCGCTACAACGACCTGATCGTCTCCGCGTTGCAGCCCAGCGGCCTC
TACCTGGCGCAGAACGTCAACAAGGCCAAGGTGCAGGGGATCGAGGCATCGTGGCGCGCCAGCCTGTGGGGCACTGACGT
GGGCGCCAGCGTGACGTTCCAGAATCCGCTGGATGAGACCAACAATACGCAGCTTCTGCGGCGTGCCCGGCGCCATGCGT
CGTTCGACGTGGGCCGGAATTTCGGAAACTGGCGTTTCGGCGGTGAATGGCTGGTATCCTCGGCGCGACTCGACAGCGGT
TCGCGCACGCTGGGCGGGTACGGTATCGTCAACCTGAACGCCCGCTACAACATCGACAAACAATGGTTCGTCGCCGCCCG
AGTGGAAAACCTGTTCGACAAGAACTACCAGCTGGCCTACCCCTACAACACGCAGGGCAGGGCCGGCTTCATCACGCTGG
GATGGCGTCAGAAGTGA

Upstream 100 bases:

>100_bases
GGGCCGGCCAAACGCCACTGAATCTGAATCGTCACGATCTGATCGGGAAGGCGGCCAGCCTGATGTCGTCAGCCCGGATA
CCGGCCTGCAGAACGAGGGT

Downstream 100 bases:

>100_bases
TGCCGCGCGCGGGGAGCCATGGCTGAGCTGCGGCGCGCGCTGATCGTCTGGGGTGTCCTCGCGCTGGCAGGGCTGGCCGT
CTTCGGCCTGTCGCTGGCGA

Product: outer membrane cobalamin receptor, TonB dependent(BtuB)

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MPRRRWHTPSPYGPRGNGPGRSLPSLLMRSTLSRSTAALKPSVRPSMPAILAAIALFPSASVFAQDATPDESSTTTPASA
SANGPAPASAPAAAPAAAQAASGQTLAPVVVTASRTAQSVTEALPHTTVVTQEDIVNSQAPDLRSLLRNQAGVEFSTNGG
MGTNTSLFMRGANSNQVLIMIDGVRISGVSNGNAQIANILPDQIDHIEVVRGNVSALYGSDAVGGVVQIFTKSGAGQAPA
ANAQVEYGSNNTRQGTVGYGGQIGATSFNVTGSMYKTDGFSAINAAQWNADNPKSAARGIGPNPANNPYENKSISGQIKH
QFTQDWDAGVATYYSNSQLSYDNAYGKPTDDYRMDSKLYTLSAFVNGKVLSDWTTHFKVAQSEDRSEGTINGLFDSLFST
RTRQFNWQNEYALTPDHTLMFGTDWLQQELASSSYNAPSRNVFSVYGGYEGRIGKHQIQLNGRNDHYSDFGDQGSFFAGY
GYYLTSAVKLLANVSNAFRAPTFNELYYPYYGQPNLQPERAKSAEVGVQVETASTGLLRVTAFETRYNDLIVSALQPSGL
YLAQNVNKAKVQGIEASWRASLWGTDVGASVTFQNPLDETNNTQLLRRARRHASFDVGRNFGNWRFGGEWLVSSARLDSG
SRTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLAYPYNTQGRAGFITLGWRQK

Sequences:

>Translated_698_residues
MPRRRWHTPSPYGPRGNGPGRSLPSLLMRSTLSRSTAALKPSVRPSMPAILAAIALFPSASVFAQDATPDESSTTTPASA
SANGPAPASAPAAAPAAAQAASGQTLAPVVVTASRTAQSVTEALPHTTVVTQEDIVNSQAPDLRSLLRNQAGVEFSTNGG
MGTNTSLFMRGANSNQVLIMIDGVRISGVSNGNAQIANILPDQIDHIEVVRGNVSALYGSDAVGGVVQIFTKSGAGQAPA
ANAQVEYGSNNTRQGTVGYGGQIGATSFNVTGSMYKTDGFSAINAAQWNADNPKSAARGIGPNPANNPYENKSISGQIKH
QFTQDWDAGVATYYSNSQLSYDNAYGKPTDDYRMDSKLYTLSAFVNGKVLSDWTTHFKVAQSEDRSEGTINGLFDSLFST
RTRQFNWQNEYALTPDHTLMFGTDWLQQELASSSYNAPSRNVFSVYGGYEGRIGKHQIQLNGRNDHYSDFGDQGSFFAGY
GYYLTSAVKLLANVSNAFRAPTFNELYYPYYGQPNLQPERAKSAEVGVQVETASTGLLRVTAFETRYNDLIVSALQPSGL
YLAQNVNKAKVQGIEASWRASLWGTDVGASVTFQNPLDETNNTQLLRRARRHASFDVGRNFGNWRFGGEWLVSSARLDSG
SRTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLAYPYNTQGRAGFITLGWRQK
>Mature_697_residues
PRRRWHTPSPYGPRGNGPGRSLPSLLMRSTLSRSTAALKPSVRPSMPAILAAIALFPSASVFAQDATPDESSTTTPASAS
ANGPAPASAPAAAPAAAQAASGQTLAPVVVTASRTAQSVTEALPHTTVVTQEDIVNSQAPDLRSLLRNQAGVEFSTNGGM
GTNTSLFMRGANSNQVLIMIDGVRISGVSNGNAQIANILPDQIDHIEVVRGNVSALYGSDAVGGVVQIFTKSGAGQAPAA
NAQVEYGSNNTRQGTVGYGGQIGATSFNVTGSMYKTDGFSAINAAQWNADNPKSAARGIGPNPANNPYENKSISGQIKHQ
FTQDWDAGVATYYSNSQLSYDNAYGKPTDDYRMDSKLYTLSAFVNGKVLSDWTTHFKVAQSEDRSEGTINGLFDSLFSTR
TRQFNWQNEYALTPDHTLMFGTDWLQQELASSSYNAPSRNVFSVYGGYEGRIGKHQIQLNGRNDHYSDFGDQGSFFAGYG
YYLTSAVKLLANVSNAFRAPTFNELYYPYYGQPNLQPERAKSAEVGVQVETASTGLLRVTAFETRYNDLIVSALQPSGLY
LAQNVNKAKVQGIEASWRASLWGTDVGASVTFQNPLDETNNTQLLRRARRHASFDVGRNFGNWRFGGEWLVSSARLDSGS
RTLGGYGIVNLNARYNIDKQWFVAARVENLFDKNYQLAYPYNTQGRAGFITLGWRQK

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=628, Percent_Identity=28.0254777070064, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788478, Length=698, Percent_Identity=25.214899713467, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1786798, Length=749, Percent_Identity=23.497997329773, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 75443; Mature: 75312

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRRWHTPSPYGPRGNGPGRSLPSLLMRSTLSRSTAALKPSVRPSMPAILAAIALFPSA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
SVFAQDATPDESSTTTPASASANGPAPASAPAAAPAAAQAASGQTLAPVVVTASRTAQSV
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCHHHHHH
TEALPHTTVVTQEDIVNSQAPDLRSLLRNQAGVEFSTNGGMGTNTSLFMRGANSNQVLIM
HHHCCCCEEEEHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCEEEEE
IDGVRISGVSNGNAQIANILPDQIDHIEVVRGNVSALYGSDAVGGVVQIFTKSGAGQAPA
ECCEEEECCCCCCCEEECCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCCCCCC
ANAQVEYGSNNTRQGTVGYGGQIGATSFNVTGSMYKTDGFSAINAAQWNADNPKSAARGI
CCCEEEECCCCCCCCCCCCCCEECCEEEECCCCEEECCCCCEEEECCCCCCCCHHHHCCC
GPNPANNPYENKSISGQIKHQFTQDWDAGVATYYSNSQLSYDNAYGKPTDDYRMDSKLYT
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEEE
LSAFVNGKVLSDWTTHFKVAQSEDRSEGTINGLFDSLFSTRTRQFNWQNEYALTPDHTLM
EEEEECCEEEECCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEE
FGTDWLQQELASSSYNAPSRNVFSVYGGYEGRIGKHQIQLNGRNDHYSDFGDQGSFFAGY
ECCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEHH
GYYLTSAVKLLANVSNAFRAPTFNELYYPYYGQPNLQPERAKSAEVGVQVETASTGLLRV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEE
TAFETRYNDLIVSALQPSGLYLAQNVNKAKVQGIEASWRASLWGTDVGASVTFQNPLDET
EEECCCCCCEEEEECCCCCEEEECCCCHHHEECCCCHHEEEECCCCCCCEEEECCCCCCC
NNTQLLRRARRHASFDVGRNFGNWRFGGEWLVSSARLDSGSRTLGGYGIVNLNARYNIDK
CCHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEECCCCCCEECCEEEEEECCEECCCH
QWFVAARVENLFDKNYQLAYPYNTQGRAGFITLGWRQK
HHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
PRRRWHTPSPYGPRGNGPGRSLPSLLMRSTLSRSTAALKPSVRPSMPAILAAIALFPSA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
SVFAQDATPDESSTTTPASASANGPAPASAPAAAPAAAQAASGQTLAPVVVTASRTAQSV
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCHHHHHH
TEALPHTTVVTQEDIVNSQAPDLRSLLRNQAGVEFSTNGGMGTNTSLFMRGANSNQVLIM
HHHCCCCEEEEHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCEEEEE
IDGVRISGVSNGNAQIANILPDQIDHIEVVRGNVSALYGSDAVGGVVQIFTKSGAGQAPA
ECCEEEECCCCCCCEEECCCCCCCCEEEEEECCEEEEECCCCCCCEEEEEECCCCCCCCC
ANAQVEYGSNNTRQGTVGYGGQIGATSFNVTGSMYKTDGFSAINAAQWNADNPKSAARGI
CCCEEEECCCCCCCCCCCCCCEECCEEEECCCCEEECCCCCEEEECCCCCCCCHHHHCCC
GPNPANNPYENKSISGQIKHQFTQDWDAGVATYYSNSQLSYDNAYGKPTDDYRMDSKLYT
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEEE
LSAFVNGKVLSDWTTHFKVAQSEDRSEGTINGLFDSLFSTRTRQFNWQNEYALTPDHTLM
EEEEECCEEEECCHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEE
FGTDWLQQELASSSYNAPSRNVFSVYGGYEGRIGKHQIQLNGRNDHYSDFGDQGSFFAGY
ECCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEHH
GYYLTSAVKLLANVSNAFRAPTFNELYYPYYGQPNLQPERAKSAEVGVQVETASTGLLRV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCEEEE
TAFETRYNDLIVSALQPSGLYLAQNVNKAKVQGIEASWRASLWGTDVGASVTFQNPLDET
EEECCCCCCEEEEECCCCCEEEECCCCHHHEECCCCHHEEEECCCCCCCEEEECCCCCCC
NNTQLLRRARRHASFDVGRNFGNWRFGGEWLVSSARLDSGSRTLGGYGIVNLNARYNIDK
CCHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEEECCCCCCEECCEEEEEECCEECCCH
QWFVAARVENLFDKNYQLAYPYNTQGRAGFITLGWRQK
HHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA