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The map label for this gene is cobQ [H]

Identifier: 94311710

GI number: 94311710

Start: 3025052

End: 3026569

Strand: Reverse

Name: cobQ [H]

Synonym: Rmet_2778

Alternate gene names: 94311710

Gene position: 3026569-3025052 (Counterclockwise)

Preceding gene: 94311715

Following gene: 94311709

Centisome position: 77.05

GC content: 68.51

Gene sequence:

>1518_bases
ATGCGCGACGAACCTGCTTCCCGAACCGATTTCCTCCCGGCTGGGCGTGCCCTGCACGGCACCCTGATGATCCAGGGCAC
CACTTCCGACGCGGGCAAGAGCACCGTCGTGGCCGGCCTTTGCCGGGTGCTGGCGCGGGCCGGGGTGCCGGTGGCGCCGT
TCAAGCCGCAGAACATGGCGCTGAACAGCGCGGTGACCGTCGATGGTGGCGAAATCGGCCGGGCGCAGGCGCTGCAGGCG
CAGGCTGCCGGACTGGCGCCCCATACCGACATGAATCCCGTCCTGCTCAAACCCAGTAGCGATACCGGCGCGCAGGTCAT
CATCCATGGCCGCGCGCGGATGGATCTGGACGCGCGTGCCTATCACGACTACAAGCCACGGGCGATGGCCGCTGTGCTGG
AAAGCCATGAGCGGCTGGCGCAGGCGTACGACGTGGTGCTGGTGGAGGGCGCGGGCAGTCCGGCGGAAGTGAACCTGCGC
GATCGCGATATCGCCAACATGGGGTTCGCGGAGCGCGTGGACTGCCCGGTGGTGATCGTGGCCGATATCGACCGGGGCGG
CGTGTTCGCGCACCTGGTCGGCACGCTCGATTGCCTGTCCGAGACGGAGCGCACGCGCGTGACCGGTTTCATCATCAACC
GCTTCCGCGGCGATATCGCGCTGCTCGAACCCGGGCTGGACTGGCTCACAGCGCGTACCGGCAAACCCGTGTTCGGGGTG
CTGCCGTATCTGCATGGCCTGCACCTCGATGCCGAGGATGCGATCGTCAGCGCCCAGGTGGCCGGGCAGGCGGGCGGGAG
CGAAGTGCTACGTGTGATCGTCCCGGTGCTGCCGCGGATTTCGAACCATACAGATTTTGACGCGCTGCGAGCCCACCCGG
GCGTCGATCTCCAGTTCATCGGCCCGGGGATGCCGATTCCGCCTGCGGATCTCGTGATCCTGCCGGGCAGCAAGAGCGTG
CGCGCGGATCTGGCCTTCCTGCGTGCCAATGGCTGGGATCGCGCGTTGCGGCGGCACCTGCGTTATGGCGGCAAGCTGAT
CGGCATCTGTGGTGGCATGCAGATGCTGGGGCGGAGCATTCACGACCCTGATGGAAGGGAGGGGCCCGCGGGCACGAGCG
ACGGCCTCGCGCTGCTCGATTTCGAAACCACGCTGGCGCCAGAAAAGCAGTTACGGCGCGTGGCGGGGCGGCTTGCCGAT
GGCAATGACGCGACGGTGCGCGGCTACGAGATTCACCTCGGGGTTACCGAGGGCCCGGCGCTGGCCCGGCCAGCGCTGTG
GCTTGAGGGTGACAGACCGGACGGCGCCCGATCCGACGATGGTCAGGTACTGGCGACCTACGTCCACGGCCTGTTCGACG
ATCCCGCCGCGTGCGGCGCGCTGCTGCGCTGGGCCGGGCTCGGTCAGGCTGAAGGCGTCGACCTCGATGCGCTGCGCGAG
GCGTCGCTCGACAGGCTGGCCGACAGCCTCGCCGCCAATCTCGATCTGGGGGCGCTGTTCGCGCCGCTGGGTCGTTAG

Upstream 100 bases:

>100_bases
CCTTGGTCTTCATGCGACGTATTGTAGCCGTCCGCATCGATCTTGCAGACTGGTGCGCCAGACCTCCCGCGCGCCAGGAC
GATGCTGCGATAATCGCGCG

Downstream 100 bases:

>100_bases
CCCGGGCGGCACGCTACACTTGCGGAGTTGTCGGAGAGTCGGGGCGGCCTGCCATGACGCCGTGCGCCCGGTGAAGTCGA
GAGTCTTGTTGTCGTTCATG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMALNSAVTVDGGEIGRAQALQA
QAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARAYHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLR
DRDIANMGFAERVDCPVVIVADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGV
LPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFIGPGMPIPPADLVILPGSKSV
RADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSIHDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLAD
GNDATVRGYEIHLGVTEGPALARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALRE
ASLDRLADSLAANLDLGALFAPLGR

Sequences:

>Translated_505_residues
MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMALNSAVTVDGGEIGRAQALQA
QAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARAYHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLR
DRDIANMGFAERVDCPVVIVADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGV
LPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFIGPGMPIPPADLVILPGSKSV
RADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSIHDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLAD
GNDATVRGYEIHLGVTEGPALARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALRE
ASLDRLADSLAANLDLGALFAPLGR
>Mature_505_residues
MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMALNSAVTVDGGEIGRAQALQA
QAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARAYHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLR
DRDIANMGFAERVDCPVVIVADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGV
LPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFIGPGMPIPPADLVILPGSKSV
RADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSIHDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLAD
GNDATVRGYEIHLGVTEGPALARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALRE
ASLDRLADSLAANLDLGALFAPLGR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 53441; Mature: 53441

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMA
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LNSAVTVDGGEIGRAQALQAQAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARA
ECCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHH
YHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLRDRDIANMGFAERVDCPVVIV
HCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCCHHCCCCHHHCCCCEEEE
ADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCHHHH
LPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFI
HHHHHCCCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEE
GPGMPIPPADLVILPGSKSVRADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSI
CCCCCCCCCCEEEECCCCCHHHHEEEEECCCHHHHHHHHHHHCCEEEEEECCHHHHCCCC
HDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLADGNDATVRGYEIHLGVTEGPA
CCCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCC
LARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALRE
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
ASLDRLADSLAANLDLGALFAPLGR
HHHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMA
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LNSAVTVDGGEIGRAQALQAQAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARA
ECCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCHHH
YHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLRDRDIANMGFAERVDCPVVIV
HCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCCHHCCCCHHHCCCCEEEE
ADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCHHHH
LPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFI
HHHHHCCCCCHHHHEEEEHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEE
GPGMPIPPADLVILPGSKSVRADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSI
CCCCCCCCCCEEEECCCCCHHHHEEEEECCCHHHHHHHHHHHCCEEEEEECCHHHHCCCC
HDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLADGNDATVRGYEIHLGVTEGPA
CCCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCC
LARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALRE
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
ASLDRLADSLAANLDLGALFAPLGR
HHHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA