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The map label for this gene is metG

Identifier: 94311701

GI number: 94311701

Start: 3011163

End: 3013235

Strand: Direct

Name: metG

Synonym: Rmet_2769

Alternate gene names: 94311701

Gene position: 3011163-3013235 (Clockwise)

Preceding gene: 94311700

Following gene: 335055630

Centisome position: 76.66

GC content: 62.81

Gene sequence:

>2073_bases
ATGACAGCACGCCGCATCCTCGTCACTTCCGCCCTGCCATATGCCAACGGCCAGATCCATATCGGCCACCTGGTGGAGTA
CATCCAGACCGACATCTGGGTGCGCTTCCAGCGCATGATGGGCAACGAGGTCTATTACGTGGGCGCCGATGACACCCACG
GCACCCCGGTCATGCTGCGCGCCGAAAAGGAAGGCATCACCCCGAAGCAGCTGATCGACCGCGTCTGGACCGAACACAAG
CGCGACTTCGACAACTTCCTGGTGTCGTTCGACAACTACTACAGCACCGACGCCGAAGAAAACCGCCAGCTTTGCGAGAA
GATCTACCTGGCCCTGAAGGCCGAGGATCTGATTACCGAGCGCGACGTCGAGCAGTTCTACGACCCGGTCAAGAACATGT
TCCTGCCGGATCGCTTCATCAAGGGCGAGTGCCCGAAGTGCGGCGCCAAGGACCAGTACGGCGATTCGTGTGAAGTTTGC
GGCACAACCTATGTCCCGACGGATCTGAAGAATCCGTACTCGGTGGTTTCCGGGGCAACGCCGGTGCGCAAGTCGTCGAC
GCACTTCTTCTTCAAGCTTTCCGACCCGCGCTGCGAAACGTTCCTGCGCGAGTGGGTGGCCGATCTGGCCCAGCCTGAAG
CCAGCAACAAGATGCAGGAATGGCTGGGTGCCGAAGGCGAAGCCTCGACGCTGTCCGATTGGGACATCTCGCGCGACGCC
CCGTACTTCGGCTTCGAGATCCCCGGCGCGCCGGGCAAGTATTTCTACGTGTGGCTCGATGCCCCGATCGGCTACTACGC
GAGCTTCAAGAACCTCGCCGAGAAGCGCGGCATCGATTTCGACGCCTGGGTCGGCCCGCACTCGACGGCAGAGCAGTACC
ACTTCATCGGCAAGGACATCCTGTACTTCCACACGCTGTTCTGGCCGGCGATGCTCCGCTTCTCGGGCTATCGCACCCCG
ACCAACGTGTTCGCGCACGGCTTCCTGACCGTCGACGGCGCCAAGATGAGCAAGTCGCGTGGCACGTTCATCACCGCGCA
GAGCTATACCGATACCGGCATGAACCCGGAATGGCTGCGCTACTACTTCGCCGCCAAGCTCAATGCGAGCATGGAAGACC
TGGACCTGAACCTGGACGACTTCGTCGCCCGCGTGAACAGCGACCTGATCGGCAAGTACGTGAACATCGCCAGCCGCGCC
GCCGGCTTCCTGGTCAAGCGCTTCGACGGCAAGGTGAACGAGGCCGCGCTGGCCAACCCGCTGCTGGAGCAACTGCGCCA
GGCCGCACCGCAACTGGCCCAGTACTATGAAGGCCGCGAGTACAGCAAGGCACTGCGTCTGGTGATGGAACTGACCGACG
CGGTCAACGCGTTCGTCGACACCAACAAGCCGTGGGAACTGGCCAAGGACGAAAGCAAGCGCGATGCGCTGCACGCCGCC
TGCTCGGTGTCGCTGGAAGCCTTCCGCCTGCTGACGATCTATCTGAAGCCGGTAGTGCCGACCATGGCCGCTGGCGTCGA
GCGTTTCCTGAACGTCGATCCGCTCGACTGGCGCGCCATCGACAAGCAGCTTTCCGCCGACCGACCGGTGCAGCCGTACC
AGCACCTGATGACGCGCGTGGATGTCAAACAGATCGATGCGCTGTTGGCAGCAAACCGCGACTCGCTGCAGGCTGCAGCG
CCGGCCGCCGAAGCTGGCGCAGCAGGCGCCTCGGCCATCGAGCCGGTTGCCGACACGATCACGATCGACGACTTCGCGAA
GATCGACCTGCGCGTGGCGAAGATCGTGGCGTGCGACAAGGTCGAAGGTTCGAACAAGCTGCTGCAGTTGACGCTGGACC
TGGGCGAAGGCAAGACGCGCAACGTGTTCTCGGGCATCCAGTCGGCCTACACGCCCGAGCAACTCGTGGGCAAGCTGACC
GTGGTGGTGGCCAACCTGGCACCGCGCAAGATGAAGTTCGGCATGTCCGAAGGCATGGTCCTGGCTGCATCGGCCGCGGA
TGAAAAGGCCAACCCGGGTCTGTACATCCTCGAACCGCACAGCGGCGCCGTGCCCGGCATGCGTATCCGCTAA

Upstream 100 bases:

>100_bases
CCGACGCGGGGCAATTGCTAAAATAGCGCCTTTACCCCCGTGCCTCCTGCCGGTCACCGCCGGCGCTCCTGGCGCAAAAC
CCCTCTGAACGGCTCTTTTC

Downstream 100 bases:

>100_bases
CCGGTAGCCGCCTCCCGGCCCGCGTGCCGGCGGTCTCCCCGATCGCCGGCACCACCTCCCCCCTCAGACCTGGTTAGCCG
CCACCCATCTTCGAAGGATC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHK
RDFDNFLVSFDNYYSTDAEENRQLCEKIYLALKAEDLITERDVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVC
GTTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEASNKMQEWLGAEGEASTLSDWDISRDA
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKRGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTP
TNVFAHGFLTVDGAKMSKSRGTFITAQSYTDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFVARVNSDLIGKYVNIASRA
AGFLVKRFDGKVNEAALANPLLEQLRQAAPQLAQYYEGREYSKALRLVMELTDAVNAFVDTNKPWELAKDESKRDALHAA
CSVSLEAFRLLTIYLKPVVPTMAAGVERFLNVDPLDWRAIDKQLSADRPVQPYQHLMTRVDVKQIDALLAANRDSLQAAA
PAAEAGAAGASAIEPVADTITIDDFAKIDLRVAKIVACDKVEGSNKLLQLTLDLGEGKTRNVFSGIQSAYTPEQLVGKLT
VVVANLAPRKMKFGMSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIR

Sequences:

>Translated_690_residues
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHK
RDFDNFLVSFDNYYSTDAEENRQLCEKIYLALKAEDLITERDVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVC
GTTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEASNKMQEWLGAEGEASTLSDWDISRDA
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKRGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTP
TNVFAHGFLTVDGAKMSKSRGTFITAQSYTDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFVARVNSDLIGKYVNIASRA
AGFLVKRFDGKVNEAALANPLLEQLRQAAPQLAQYYEGREYSKALRLVMELTDAVNAFVDTNKPWELAKDESKRDALHAA
CSVSLEAFRLLTIYLKPVVPTMAAGVERFLNVDPLDWRAIDKQLSADRPVQPYQHLMTRVDVKQIDALLAANRDSLQAAA
PAAEAGAAGASAIEPVADTITIDDFAKIDLRVAKIVACDKVEGSNKLLQLTLDLGEGKTRNVFSGIQSAYTPEQLVGKLT
VVVANLAPRKMKFGMSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIR
>Mature_689_residues
TARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHKR
DFDNFLVSFDNYYSTDAEENRQLCEKIYLALKAEDLITERDVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCG
TTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEASNKMQEWLGAEGEASTLSDWDISRDAP
YFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKRGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTPT
NVFAHGFLTVDGAKMSKSRGTFITAQSYTDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFVARVNSDLIGKYVNIASRAA
GFLVKRFDGKVNEAALANPLLEQLRQAAPQLAQYYEGREYSKALRLVMELTDAVNAFVDTNKPWELAKDESKRDALHAAC
SVSLEAFRLLTIYLKPVVPTMAAGVERFLNVDPLDWRAIDKQLSADRPVQPYQHLMTRVDVKQIDALLAANRDSLQAAAP
AAEAGAAGASAIEPVADTITIDDFAKIDLRVAKIVACDKVEGSNKLLQLTLDLGEGKTRNVFSGIQSAYTPEQLVGKLTV
VVANLAPRKMKFGMSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=525, Percent_Identity=28.5714285714286, Blast_Score=223, Evalue=5e-58,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=26.5420560747664, Blast_Score=146, Evalue=8e-35,
Organism=Homo sapiens, GI4507947, Length=104, Percent_Identity=35.5769230769231, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788432, Length=694, Percent_Identity=54.178674351585, Blast_Score=754, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=517, Percent_Identity=29.4003868471954, Blast_Score=237, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI212645096, Length=529, Percent_Identity=23.8185255198488, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=331, Percent_Identity=25.6797583081571, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=27.3703041144902, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6321610, Length=547, Percent_Identity=22.8519195612431, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24655311, Length=546, Percent_Identity=29.4871794871795, Blast_Score=210, Evalue=3e-54,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=25.9117082533589, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_RALME (Q1LJN4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584911.1
- ProteinModelPortal:   Q1LJN4
- STRING:   Q1LJN4
- GeneID:   4039595
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2769
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   Q1LJN4
- ProtClustDB:   PRK00133
- BioCyc:   RMET266264:RMET_2769-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77187; Mature: 77056

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 338-338

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLR
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEE
AEKEGITPKQLIDRVWTEHKRDFDNFLVSFDNYYSTDAEENRQLCEKIYLALKAEDLITE
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCGTTYVPTDLKNPYSVVSGAT
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PVRKSSTHFFFKLSDPRCETFLREWVADLAQPEASNKMQEWLGAEGEASTLSDWDISRDA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKRGIDFDAWVGPHSTAEQYHFIGKDI
CCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYFHTLFWPAMLRFSGYRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYTDTGMNPEWLR
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHH
YYFAAKLNASMEDLDLNLDDFVARVNSDLIGKYVNIASRAAGFLVKRFDGKVNEAALANP
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLEQLRQAAPQLAQYYEGREYSKALRLVMELTDAVNAFVDTNKPWELAKDESKRDALHAA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
CSVSLEAFRLLTIYLKPVVPTMAAGVERFLNVDPLDWRAIDKQLSADRPVQPYQHLMTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
DVKQIDALLAANRDSLQAAAPAAEAGAAGASAIEPVADTITIDDFAKIDLRVAKIVACDK
HHHHHHHHHHCCCHHHHHHCCCHHCCCCCCHHHHHHHHEEEECCHHHHHHHHHHHEEECC
VEGSNKLLQLTLDLGEGKTRNVFSGIQSAYTPEQLVGKLTVVVANLAPRKMKFGMSEGMV
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCEE
LAASAADEKANPGLYILEPHSGAVPGMRIR
EEEECCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLR
CCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEE
AEKEGITPKQLIDRVWTEHKRDFDNFLVSFDNYYSTDAEENRQLCEKIYLALKAEDLITE
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCGTTYVPTDLKNPYSVVSGAT
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PVRKSSTHFFFKLSDPRCETFLREWVADLAQPEASNKMQEWLGAEGEASTLSDWDISRDA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKRGIDFDAWVGPHSTAEQYHFIGKDI
CCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYFHTLFWPAMLRFSGYRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYTDTGMNPEWLR
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHH
YYFAAKLNASMEDLDLNLDDFVARVNSDLIGKYVNIASRAAGFLVKRFDGKVNEAALANP
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLEQLRQAAPQLAQYYEGREYSKALRLVMELTDAVNAFVDTNKPWELAKDESKRDALHAA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
CSVSLEAFRLLTIYLKPVVPTMAAGVERFLNVDPLDWRAIDKQLSADRPVQPYQHLMTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
DVKQIDALLAANRDSLQAAAPAAEAGAAGASAIEPVADTITIDDFAKIDLRVAKIVACDK
HHHHHHHHHHCCCHHHHHHCCCHHCCCCCCHHHHHHHHEEEECCHHHHHHHHHHHEEECC
VEGSNKLLQLTLDLGEGKTRNVFSGIQSAYTPEQLVGKLTVVVANLAPRKMKFGMSEGMV
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCEE
LAASAADEKANPGLYILEPHSGAVPGMRIR
EEEECCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA