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The map label for this gene is rsuA [H]

Identifier: 94311676

GI number: 94311676

Start: 2978840

End: 2979556

Strand: Reverse

Name: rsuA [H]

Synonym: Rmet_2744

Alternate gene names: 94311676

Gene position: 2979556-2978840 (Counterclockwise)

Preceding gene: 94311679

Following gene: 94311675

Centisome position: 75.85

GC content: 66.53

Gene sequence:

>717_bases
ATGGCCACGACACTCGACCGCATCCTGCAATCCCAAGGCTTCGGCACCCGCCGCTATTGCGGCGATCTGGTGGCGGCCGG
GCTGGTCGAGGTCAACGGTGAACTCTGCGAGGACCCGGGTGCGCGTTTCGAGGTGGACGGGCTGCGCCTGATGGTCGACG
GCGAGGAGTGGCTGGCGTGCACCAAGGCCTACGTGATGCTGAACAAGCCGACGGGCTACGAATGCTCCCAGCGTCCGCGC
CATCATCCAAGTGTGTACAACCTGCTGCCGGTACCGCTGCGCCAGCGGGAGGTCCAGGCTGTGGGGCGGCTCGATCACGA
CACCACCGGATTGCTGCTGCTGACCGACGACGGTCAGTTCATCCACGCCCAGACCTCGCCGAAGAAGCAGGTGCCGAAGA
TTTACGAAGTGACGACCTTCGACCCGGTTACGCCGGAACAGGTGGAAGCGTTGCGCACTGGCGTGAAGCTCGACGACGAA
CCGGCGCCGATTGCCGCCGATGCGTGCGAAGCGACCGGCGAGCGCAGCCTGCGTCTGACACTGCTGCAGGGCAAGTACCA
TCAGGTCAAGCGGATGGTCGCTGCGGTGGGTAACCACGTGACCGCGTTGCATCGCAGCGCGATCGGCGGACTCGTGCTCG
ACCCCGCGCTGGGCCCCGGAGAATGGCGCTGGCTCACGCCGGAAGACCTGGCCGCGCTGCGCGACGGAGCGCGTTGA

Upstream 100 bases:

>100_bases
TCGGCTTCAGATTCGGGGGAGGGCGATCCAGACATCGCCGTAACCTAACATAGCAACCGGAACTCCAGCAGTCCCATCCA
AGCAGCAGAAAGATATTCCC

Downstream 100 bases:

>100_bases
GGTAGCAACGAATGCTGCGTTGCAGCATGTCGCCGGACGCCGAAGTTTTACGGGAAGTCGCAGCGGTGATTTGGTAGAAT
GCAAGCCATGGCGACGTCCG

Product: 16S rRNA pseudouridylate 516 synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLACTKAYVMLNKPTGYECSQRPR
HHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDE
PAPIAADACEATGERSLRLTLLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR

Sequences:

>Translated_238_residues
MATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLACTKAYVMLNKPTGYECSQRPR
HHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDE
PAPIAADACEATGERSLRLTLLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR
>Mature_237_residues
ATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLACTKAYVMLNKPTGYECSQRPRH
HPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDEP
APIAADACEATGERSLRLTLLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=41.6309012875536, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=33.8775510204082, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=39.041095890411, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26158; Mature: 26027

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLAC
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCEEEEECCHHHHHH
TKAYVMLNKPTGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQF
EEEEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCE
IHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDEPAPIAADACEATGERSLRLT
EEECCCCHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEE
LLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR
EECCHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCEEEECHHHHHHHHCCCC
>Mature Secondary Structure 
ATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLAC
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEECCEEEEECCHHHHHH
TKAYVMLNKPTGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQF
EEEEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCE
IHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDEPAPIAADACEATGERSLRLT
EEECCCCHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEE
LLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR
EECCHHHHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCEEEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]