Click here to switch to the map view.

The map label for this gene is rfbA [H]

Identifier: 94311665

GI number: 94311665

Start: 2968611

End: 2969489

Strand: Reverse

Name: rfbA [H]

Synonym: Rmet_2733

Alternate gene names: 94311665

Gene position: 2969489-2968611 (Counterclockwise)

Preceding gene: 94311666

Following gene: 94311664

Centisome position: 75.6

GC content: 57.34

Gene sequence:

>879_bases
ATGCGCAAAGGCATTATTCTGGCCGGCGGTTCCGGCACTCGGCTCTATCCCATCACGCGGTCGGTGTCCAAACAACTGCT
GCCCGTGTACGACAAGCCGATGATTTACTATCCGCTGTCCACGCTGATGCTGGCCGGGATTCGGGACGTCCTTATCATCT
CCACGCCGGAAGACACGCCTCGCTTCTCGGAAATGCTTGGTGATGGCAGCAATTGGGGCATCAATCTGCAATACGCGGTG
CAACCGTCGCCGGATGGGCTTGCCCAGGCGTTCATCATTGGGCGCGATTTCATCGGTAACGACCCGTCGACGTTGATCCT
GGGGGATAACATCTTCCACGGACACGACCTGGTTCGTCAGCTCGAGCGTTCGTCAGCACAGCAGCAGGGCGCGACGGTCT
TTGCCTATCACGTGCACGACCCCGAGCGCTATGGCGTGGTGGAGTTCGATGAAAACTTTCGCGCGGTGTCGCTCGAGGAA
AAACCAGCGAAGCCTCGCTCGCACTATGCCGTGACCGGCCTGTATTTCTACGACAACCAGGTTTGTGATATTGCCGCCGA
GATCAAGCCGTCAGCGCGGGGCGAACTCGAGATTACGGATGTCAACAAGCGCTATCTGGAAATGTCGCAGCTTGAGGTCG
AGATCATGGGACGCGGTTACGCATGGCTCGATACCGGCACCCACGACTCGTTGCTCGAGGCAGCGAGCTTCATAGCCACG
CTGCAGAACCGTCAGGGACTGATGGTGGCTTGTCCGGAGGAGATTGCCTATCGGAGCAAGTGGATTTCCGCCGAGCAGGT
TGAGCGTCTGGCGAAGCCACTGCTAAAGAACGCTTACGGCAAGTATCTTCAGCACATCATTTCGGAAACAATCAAATGA

Upstream 100 bases:

>100_bases
ATACCGGCAAGCTGCGGGACACATTCAATATTCACCTTCCTGACTGGGCGGAGGGCATTGACCTTCTTCTCGATCAGATC
CTTTCCTGACCACTTGTACC

Downstream 100 bases:

>100_bases
GTTTGACCGTTACTCGTACCGCCATTCCGGACGTGATGATCATCGAGCCGAAGGTGTTCGGTGATGCCCGGGGGTTTTTC
TTTGAGAGCTTCAATCAGAA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRKGIILAGGSGTRLYPITRSVSKQLLPVYDKPMIYYPLSTLMLAGIRDVLIISTPEDTPRFSEMLGDGSNWGINLQYAV
QPSPDGLAQAFIIGRDFIGNDPSTLILGDNIFHGHDLVRQLERSSAQQQGATVFAYHVHDPERYGVVEFDENFRAVSLEE
KPAKPRSHYAVTGLYFYDNQVCDIAAEIKPSARGELEITDVNKRYLEMSQLEVEIMGRGYAWLDTGTHDSLLEAASFIAT
LQNRQGLMVACPEEIAYRSKWISAEQVERLAKPLLKNAYGKYLQHIISETIK

Sequences:

>Translated_292_residues
MRKGIILAGGSGTRLYPITRSVSKQLLPVYDKPMIYYPLSTLMLAGIRDVLIISTPEDTPRFSEMLGDGSNWGINLQYAV
QPSPDGLAQAFIIGRDFIGNDPSTLILGDNIFHGHDLVRQLERSSAQQQGATVFAYHVHDPERYGVVEFDENFRAVSLEE
KPAKPRSHYAVTGLYFYDNQVCDIAAEIKPSARGELEITDVNKRYLEMSQLEVEIMGRGYAWLDTGTHDSLLEAASFIAT
LQNRQGLMVACPEEIAYRSKWISAEQVERLAKPLLKNAYGKYLQHIISETIK
>Mature_292_residues
MRKGIILAGGSGTRLYPITRSVSKQLLPVYDKPMIYYPLSTLMLAGIRDVLIISTPEDTPRFSEMLGDGSNWGINLQYAV
QPSPDGLAQAFIIGRDFIGNDPSTLILGDNIFHGHDLVRQLERSSAQQQGATVFAYHVHDPERYGVVEFDENFRAVSLEE
KPAKPRSHYAVTGLYFYDNQVCDIAAEIKPSARGELEITDVNKRYLEMSQLEVEIMGRGYAWLDTGTHDSLLEAASFIAT
LQNRQGLMVACPEEIAYRSKWISAEQVERLAKPLLKNAYGKYLQHIISETIK

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=249, Percent_Identity=26.1044176706827, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI11761619, Length=249, Percent_Identity=26.1044176706827, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788351, Length=290, Percent_Identity=71.0344827586207, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=64.2105263157895, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI133931050, Length=242, Percent_Identity=24.3801652892562, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=274, Percent_Identity=24.8175182481752, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355443, Length=246, Percent_Identity=25.609756097561, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644084, Length=246, Percent_Identity=25.609756097561, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32745; Mature: 32745

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGIILAGGSGTRLYPITRSVSKQLLPVYDKPMIYYPLSTLMLAGIRDVLIISTPEDTP
CCCCEEEECCCCCEEEEEHHHHHHHHCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCH
RFSEMLGDGSNWGINLQYAVQPSPDGLAQAFIIGRDFIGNDPSTLILGDNIFHGHDLVRQ
HHHHHHCCCCCCCEEEEEEECCCCCHHHEEEEEECHHCCCCCCEEEEECCEECHHHHHHH
LERSSAQQQGATVFAYHVHDPERYGVVEFDENFRAVSLEEKPAKPRSHYAVTGLYFYDNQ
HHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCC
VCDIAAEIKPSARGELEITDVNKRYLEMSQLEVEIMGRGYAWLDTGTHDSLLEAASFIAT
EEEEEECCCCCCCCCEEEEECCHHHHHHHHEEEEEEECCEEEEECCCCHHHHHHHHHHHH
LQNRQGLMVACPEEIAYRSKWISAEQVERLAKPLLKNAYGKYLQHIISETIK
HHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKGIILAGGSGTRLYPITRSVSKQLLPVYDKPMIYYPLSTLMLAGIRDVLIISTPEDTP
CCCCEEEECCCCCEEEEEHHHHHHHHCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCH
RFSEMLGDGSNWGINLQYAVQPSPDGLAQAFIIGRDFIGNDPSTLILGDNIFHGHDLVRQ
HHHHHHCCCCCCCEEEEEEECCCCCHHHEEEEEECHHCCCCCCEEEEECCEECHHHHHHH
LERSSAQQQGATVFAYHVHDPERYGVVEFDENFRAVSLEEKPAKPRSHYAVTGLYFYDNQ
HHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEECCC
VCDIAAEIKPSARGELEITDVNKRYLEMSQLEVEIMGRGYAWLDTGTHDSLLEAASFIAT
EEEEEECCCCCCCCCEEEEECCHHHHHHHHEEEEEEECCEEEEECCCCHHHHHHHHHHHH
LQNRQGLMVACPEEIAYRSKWISAEQVERLAKPLLKNAYGKYLQHIISETIK
HHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]