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The map label for this gene is tolB

Identifier: 94311607

GI number: 94311607

Start: 2908503

End: 2909843

Strand: Reverse

Name: tolB

Synonym: Rmet_2675

Alternate gene names: 94311607

Gene position: 2909843-2908503 (Counterclockwise)

Preceding gene: 94311608

Following gene: 94311606

Centisome position: 74.08

GC content: 65.18

Gene sequence:

>1341_bases
ATGCGAAAGCTTTGGGCCCCCAACTGGCTATCCCGACGCCAGAACGCCAATCCCACCCGCGACCAGAGTCGCCATGCCTT
GATGGCGTGGCTGGCTGCGGCATTGATGTCGGCGGGCGCGGCGCACGCGCAACTGAACGTGGAGATCACCGGAGTCGGCT
CGAACCAGTTCCCGGTAGCGACCGCCAACTTCCAGGGCGAGGCCCAGGCCCCGCAGAACCTGACCGCCATCATCCGCAGC
GACCTGACGAACAGCGGACGCTTCCGCAACGTCGACCCCGCAGGTGCGACCGTGGCCGAATCGGCCCAGGTGGACCTGGG
CAGCTGGAAGGCGAAGGGCGCCGACGCGTTCGTGGCCGGCAGCGTGACGCCGACCAGCAATGGCCAGTACGAGGTGCGTT
TCCGCCTGTACGACACCGCCAAGGGGCAGAGCCTTGGCGGTCTGGCATTCACCGTCACGCAGGGCCAGCTCCGCGTGACC
GCGCACAAGATCGCCGACTACATCTACGAGAAGCTGCTCGGCGAGCGCGGCGTGTTTGCGACGCGCCTGTCGTATGTGTC
GAAGGTGGGCAATCGCTACCAGCTTCTGATCTCCGATTCGGATGGCCAGAACGCCCAGATCGCGCTGACCAGCACCGAAC
CGATCATCTCCCCCGCGTGGTCGCCCGATGGTCGCCGCGTGGCCTACGTCTCGTTCGAAGCCAAGAAGCCGGTGGTCTAC
GTGCATGACCTGGCAACGGGCAAGCGTGTTGTGGTGTCGAATCAGAAGGGCAACAACAGCGCGCCGTCCTGGTCGCCCGA
TGGCCAGCACCTGGCCGTGTCGCTCTCCCGCGACGGCAACACGCAGATCTACCAGGTCAACGCCGATGGCTCAGGCCTGC
GTCGCCTGACCCGCAGCAGCGCCATCGATACGGAGCCGCAGTTCTCCCCGGATGGCCGTAGCATTTATTTCACCAGCGAT
CGCGGCGGCGCGCCGCAGATCTACCGTATGCCGGCGTCTGGCGAGGAATCCGGCGCAGCGCAGCGCGTGACCTTCAAGGG
CAGCTACAACGTCAGCCCGCGTATATCGCCGGACGGCAAGTACCTGGCCTACATTTCGCGCAGCGGTGGCTTCAGGCTGC
AACTACAGGACCTGTCGAACGGTGATGTGACGTCGCTCACCGATACGACCAACGATGAATCCCCGAGCTTCGCGGCCAAC
GGCAAGTACATTCTCTATGCCACCCGCGTGGGCGGCCGCTCCGTACTGGCGGCAGTCTCCACGGACGGACGCACCAAGCA
GGTTCTGTCTCTCCAGTCCGGCGCTGTTCGAGAGCCGTCCTGGGGGCCTTTCATGCAGTAA

Upstream 100 bases:

>100_bases
TACGTACGCTTGTGGGGCCATGCCAAGCCGCATGTCGCCCGTCTCGCCGTGTTTGCTGTGCCGCCGCAACGATGAAACCT
GAAATCGACTGAGGAGCAGC

Downstream 100 bases:

>100_bases
AAAAGAGGAGTCTCTCGCCATGTCCCAAATGATGACCAAATCGCTCGCCATTGCCGCTCTGCTGGCTCTGGGCGCCTGCA
GCTCGGGTGTGAAGCTCGAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVATANFQGEAQAPQNLTAIIRS
DLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAGSVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVT
AHKIADYIYEKLLGERGVFATRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVY
VHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPQFSPDGRSIYFTSD
RGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGKYLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAAN
GKYILYATRVGGRSVLAAVSTDGRTKQVLSLQSGAVREPSWGPFMQ

Sequences:

>Translated_446_residues
MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVATANFQGEAQAPQNLTAIIRS
DLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAGSVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVT
AHKIADYIYEKLLGERGVFATRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVY
VHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPQFSPDGRSIYFTSD
RGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGKYLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAAN
GKYILYATRVGGRSVLAAVSTDGRTKQVLSLQSGAVREPSWGPFMQ
>Mature_446_residues
MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVATANFQGEAQAPQNLTAIIRS
DLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAGSVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVT
AHKIADYIYEKLLGERGVFATRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVY
VHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPQFSPDGRSIYFTSD
RGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGKYLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAAN
GKYILYATRVGGRSVLAAVSTDGRTKQVLSLQSGAVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=433, Percent_Identity=37.1824480369515, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RALME (Q1LJX8)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584817.1
- ProteinModelPortal:   Q1LJX8
- SMR:   Q1LJX8
- STRING:   Q1LJX8
- GeneID:   4039500
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2675
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q1LJX8
- ProtClustDB:   PRK02889
- BioCyc:   RMET266264:RMET_2675-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48010; Mature: 48010

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEE
TANFQGEAQAPQNLTAIIRSDLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAG
EECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEE
SVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVTAHKIADYIYEKLLGERGVFA
CCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHCCCCHHH
TRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVY
HHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEE
VHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSS
EEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHC
AIDTEPQFSPDGRSIYFTSDRGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGK
CCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEECCCCC
YLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAANGKYILYATRVGGRSVLAAVS
EEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCCEECCCCEEEEEEECCCCEEEEEEC
TDGRTKQVLSLQSGAVREPSWGPFMQ
CCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVA
CCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEE
TANFQGEAQAPQNLTAIIRSDLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAG
EECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEE
SVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVTAHKIADYIYEKLLGERGVFA
CCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHCCCCHHH
TRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVY
HHHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEE
VHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSS
EEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHC
AIDTEPQFSPDGRSIYFTSDRGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGK
CCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEECCCCC
YLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAANGKYILYATRVGGRSVLAAVS
EEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCCCEECCCCEEEEEEECCCCEEEEEEC
TDGRTKQVLSLQSGAVREPSWGPFMQ
CCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA