Click here to switch to the map view.

The map label for this gene is cfr9IM [H]

Identifier: 94311602

GI number: 94311602

Start: 2903604

End: 2904770

Strand: Reverse

Name: cfr9IM [H]

Synonym: Rmet_2670

Alternate gene names: 94311602

Gene position: 2904770-2903604 (Counterclockwise)

Preceding gene: 335055624

Following gene: 94311601

Centisome position: 73.95

GC content: 55.36

Gene sequence:

>1167_bases
TTGATGACCACCAAACCAGAGTCCTCAGTTTTCTCGAACGTCTCGGCCCCCGAGCTGCTCCGTTATCACTCTGATGCGAG
GCACATCCCAGTCCCTGATGCCTCTGTCGATCTGATTGTGACTTCTCCCCCATACTGGAAGAAGCGGGACTACGGTTTCG
ATGGGCAAATTGGCCAGGAGTCCACGCCTGCAGCCTACGTCGCGAACATGATCGAATGCATGAGAGAGTGGCGACGTGTA
CTTAAGCCGTCGGGCTCCATATTTCTCAATGTTGGGGATTCCTATATGGCGCGCACTCTTGCAGGGGTGCCGGGGCGCTT
GGAGGCTGCTGCTATTGACGACGGGTGGTTGGTTCGAAATCGAATTATCTGGGCAAAGGAAAGCGGTATGCCCGAACCTG
CAAAGAATCGCCTTGCCAATCGGCATGAGTACATCATTCACTTCGTCAGTTCTGCCAAGTATTTTTACGATTTGGCCGGC
TATGCAGAAGCGAATGGCAATGGTGCCAATCCGGGTGACGTGTGGAATATTCCCCTTCGGCGCAACATGGGCGATCACCT
AGCGCCGTTCCCGAATGAGATTGTCCGAAGGGCGATCCTGTTGGCTTGCCCCATAGCAACGTGCCCAAAGTGCGGTCACA
TTCCCGAGCGCGTGGTCCAACGTACGGCAGTGCTGGATGAGAGCAGGCCGCAAGCTCGGCGCGCGATGGAAATTGCACGC
GAAGCCGGATTGACCGATGCCCATATCGCAGCCGTGCAGGCCACCGGTATCTCAGATGCGGGCAAGGCCCTACGTGTTCA
AACCGGTACCGGTCGGAACTCGCGAGAAGTACAGCGATTGGCTGCAGAAGCTAAAGAGGTCTTGGGTGGCTACTTCCGCG
AATTCACGTTCGCAAAGAAGCAAACTGTTGGCTGGACGACATGCTCATGCAAAGGGAAGCTCAAACCTGGGGTTGTTCTC
GATCCATTTATGGGCACGGGTACGACGCTGTCAACGGCGCTGGAAATGGGGAGATCGGCCATCGGTGTTGATCTGGCTCC
GAAAACGGACGATCATGTATGGGGTGCCGATGCAGAATCCCTAGAAAATGTCGACCGTGCCGTCAAACCAGTTAGTGTTA
AGCGTAGAAGCCGTTCAGATAGCTCTCGCCTCGCTTTGCAAGAATAG

Upstream 100 bases:

>100_bases
TCGACGAGCGGCACGTGACTAGCTATAATTTCTCCCGCTTGCCCGCGCCTGTTCAAGTGCAGAATGCGGCGCCCCACCCT
CCGTCGCTTAGGTTCTCGTC

Downstream 100 bases:

>100_bases
GATACACCCGTCTTTCGCTGGGTATCTCTGTCTAAAAGAGAGTGCTAGAGCGAGCGGCGAGCATGTAAGCCTCAAACCTA
GCTTCAAAGGTTTTTTTGAG

Product: putative DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.Cfr9I; N(4)- cytosine-specific methyltransferase Cfr9I [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQESTPAAYVANMIECMREWRRV
LKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRNRIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAG
YAEANGNGANPGDVWNIPLRRNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAR
EAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKKQTVGWTTCSCKGKLKPGVVL
DPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAESLENVDRAVKPVSVKRRSRSDSSRLALQE

Sequences:

>Translated_388_residues
MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQESTPAAYVANMIECMREWRRV
LKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRNRIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAG
YAEANGNGANPGDVWNIPLRRNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAR
EAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKKQTVGWTTCSCKGKLKPGVVL
DPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAESLENVDRAVKPVSVKRRSRSDSSRLALQE
>Mature_388_residues
MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQESTPAAYVANMIECMREWRRV
LKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRNRIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAG
YAEANGNGANPGDVWNIPLRRNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAR
EAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKKQTVGWTTCSCKGKLKPGVVL
DPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAESLENVDRAVKPVSVKRRSRSDSSRLALQE

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the Cfr9I endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 42562; Mature: 42562

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQE
CCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
STPAAYVANMIECMREWRRVLKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRN
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCEEEECCCCEEEEE
RIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAGYAEANGNGANPGDVWNIPLR
EEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCHH
RNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAR
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
EAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKK
HCCCCHHHEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVGWTTCSCKGKLKPGVVLDPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAES
CCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHH
LENVDRAVKPVSVKRRSRSDSSRLALQE
HHHHHHHHCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQE
CCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
STPAAYVANMIECMREWRRVLKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRN
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCEEEECCCCEEEEE
RIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAGYAEANGNGANPGDVWNIPLR
EEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCHH
RNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAR
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
EAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKK
HCCCCHHHEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVGWTTCSCKGKLKPGVVLDPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAES
CCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCHHH
LENVDRAVKPVSVKRRSRSDSSRLALQE
HHHHHHHHCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2690010; 8163180 [H]