| Definition | Cupriavidus metallidurans CH34 chromosome, complete genome. |
|---|---|
| Accession | NC_007973 |
| Length | 3,928,089 |
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The map label for this gene is dnaG [H]
Identifier: 94311543
GI number: 94311543
Start: 2836513
End: 2838354
Strand: Reverse
Name: dnaG [H]
Synonym: Rmet_2607
Alternate gene names: 94311543
Gene position: 2838354-2836513 (Counterclockwise)
Preceding gene: 94311544
Following gene: 94311542
Centisome position: 72.26
GC content: 65.26
Gene sequence:
>1842_bases GTGATTCCGCAATCCTTTATTCAGGACCTGCTCAACCGCGTCGACATTGTCGATGTGGTGGGCAAGTACGTGCAGTTGAA GAAGGGCGGCGCCAACTTCATGGGCCTGTGCCCGTTCCATAACGAGAAGTCCCCGTCCTTTACCGTATCGCCGACCAAGC AGTTCTATCACTGCTTCGGCTGCGGCGCACATGGATCCGCCATCGGGTTCCTGATGGAGTACTCGGGGCAGTCCTACCCC GACGCGGTGCGTGAGCTGGCCCAGTCGGTCGGCCTGTCCGTGCCGGAGGAGCGCAATAGTCTGCCACCCGCCCAGCGGGC AGAGCAGCAGGCCAGGTCGTTGGCGCTGACCGATGCCATGACGCGTGCCACCGACTTCTACAAGAAGCAGTTGCGCGGTG CCCCGCAGGCCATCCAGTACCTCAAGGGCCGTGGCCTGACCGGCGAGATTGCCGCTCACTTCGGTCTTGGCTACGCGCCG GACGACTGGCAAGGGCTGGAGACCGTGTTCGGAAGCTATCGCGATGACGCCATCGCCGGTCCGCTGGTGGAGGCCGGGCT GCTGATCGAGTCCCAGGAGAAGCGCGACGCCGATGGCAAGCCGCGTCGGTACGACCGCTTCCGCGATCGCATCATGTTCC CGATTCGCAATACGAAGGGCAATGTGATCGGGTTTGGCGGCCGCGTGATGGGGCAGGGCGAGCCAAAGTACCTGAATTCA CCGGAAACGCCGCTCTTCAGCAAGGGAACGGAGCTCTACGGTCTGTTCGAGGCGCGTCACGCAATCCGCGAAAATGGCTA CGTGCTGGTGGTAGAAGGCTACATGGATGTGGTCGCGCTGGCCCAGCTCGGGTTTGCCAATGCCGTCGCCACACTCGGCA CGGCCTGCACGCCAGTTCATGTGCAGAAGCTGATGCGCCAGACCGACGCCGTGGTCTTCTCGTTCGACGGTGACGCCGCT GGCCGCCGTGCCGCGCGCCGCGCGTTGGAAGCCTGCCTGCCGCACGTGGCGGATAACAAGACGATCCGCTTCCTTTTTCT GCCGGCCGAACACGACCCGGATAGCTATGTCCGCGAGGAGGGAACTGACGCGTTCGCGCGCCAGGTTCAGAACGCGATGC CTTTGTCGCGCTTCTTGCTGCAAATCGTCACGGAAGATCAGGATCTGCGCCAGCCCGAGGGCCGTGCCCGCGCGCAGTAC GAGGCCAAGCCGCTGCTTCAGGCGATGCCCGCCGGTGGCTTGCGACTGCAGATCGTGCGCGAACTTGCCGATACCACAAG CACCACGCCGGCCGAGATCGAGGCGATTTGCGGCCTGGGCAGCGATTCCTCGCGGGTTGGTCGTTTCGCCCAGCCGCGCC CGCGCGCGCGGCGTCAGGCGCCGACCGGGCTCGAGCAGCGCGTGATCCAGTTGCTGATGTGCTACCCGTCGCTGGCGGCC CGGCTCGACGGGGACGCGCGCGCATTGCTGCTGGACCCCCAGCACTCCGGATCCGAGGCGCTGGGCTACTTGGTGTCCGC TTGCGATGGCATTCAGGGTGAGGTGAACTTTGCGGCCTTCAGCGAGCAGTTGGCGCAGACGCCCTACGCCGATGTCTACG CGAGCGCACGCACGGCGGTACTGCGCGAGGAAATCGAAGAGGAGCCGGCGACGCATGAGTTCGACGGCGCCGTCCACAAG TTGCTGGCCGAGCCGCTGGAGCGCGAGCAGAAGGCGCTCGAGGCCATGGTGAGCGCGGGCACGGCTGACGAAGAGACCAA GGCGCGATATCTCTGGCTATCGCGCGAAGTGCAGCGGCGCAGGCAAATGGGCTGGCGGCCCGCCGCCATGGAGGATAGTT GA
Upstream 100 bases:
>100_bases GATAGCCGCCGGGCGACGCCCCGGTCACGTATTCCAGAAAACTTCGAAACGGCCGCCGGCGTTTTGCCCGGCAGTCCGTG CCATCAGGACGGTCAGTCGG
Downstream 100 bases:
>100_bases ACCGATTTTCGGCGTGAGCAGTTGAGCACGTGGCCAGAACCGATGGAAAAATTGGCGGCCAAACTGCTTAAGCCTTGATT TTTCAGGTGAAAACACAACC
Product: DNA primase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 613; Mature: 613
Protein sequence:
>613_residues MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP DAVRELAQSVGLSVPEERNSLPPAQRAEQQARSLALTDAMTRATDFYKKQLRGAPQAIQYLKGRGLTGEIAAHFGLGYAP DDWQGLETVFGSYRDDAIAGPLVEAGLLIESQEKRDADGKPRRYDRFRDRIMFPIRNTKGNVIGFGGRVMGQGEPKYLNS PETPLFSKGTELYGLFEARHAIRENGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLMRQTDAVVFSFDGDAA GRRAARRALEACLPHVADNKTIRFLFLPAEHDPDSYVREEGTDAFARQVQNAMPLSRFLLQIVTEDQDLRQPEGRARAQY EAKPLLQAMPAGGLRLQIVRELADTTSTTPAEIEAICGLGSDSSRVGRFAQPRPRARRQAPTGLEQRVIQLLMCYPSLAA RLDGDARALLLDPQHSGSEALGYLVSACDGIQGEVNFAAFSEQLAQTPYADVYASARTAVLREEIEEEPATHEFDGAVHK LLAEPLEREQKALEAMVSAGTADEETKARYLWLSREVQRRRQMGWRPAAMEDS
Sequences:
>Translated_613_residues MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP DAVRELAQSVGLSVPEERNSLPPAQRAEQQARSLALTDAMTRATDFYKKQLRGAPQAIQYLKGRGLTGEIAAHFGLGYAP DDWQGLETVFGSYRDDAIAGPLVEAGLLIESQEKRDADGKPRRYDRFRDRIMFPIRNTKGNVIGFGGRVMGQGEPKYLNS PETPLFSKGTELYGLFEARHAIRENGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLMRQTDAVVFSFDGDAA GRRAARRALEACLPHVADNKTIRFLFLPAEHDPDSYVREEGTDAFARQVQNAMPLSRFLLQIVTEDQDLRQPEGRARAQY EAKPLLQAMPAGGLRLQIVRELADTTSTTPAEIEAICGLGSDSSRVGRFAQPRPRARRQAPTGLEQRVIQLLMCYPSLAA RLDGDARALLLDPQHSGSEALGYLVSACDGIQGEVNFAAFSEQLAQTPYADVYASARTAVLREEIEEEPATHEFDGAVHK LLAEPLEREQKALEAMVSAGTADEETKARYLWLSREVQRRRQMGWRPAAMEDS >Mature_613_residues MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP DAVRELAQSVGLSVPEERNSLPPAQRAEQQARSLALTDAMTRATDFYKKQLRGAPQAIQYLKGRGLTGEIAAHFGLGYAP DDWQGLETVFGSYRDDAIAGPLVEAGLLIESQEKRDADGKPRRYDRFRDRIMFPIRNTKGNVIGFGGRVMGQGEPKYLNS PETPLFSKGTELYGLFEARHAIRENGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLMRQTDAVVFSFDGDAA GRRAARRALEACLPHVADNKTIRFLFLPAEHDPDSYVREEGTDAFARQVQNAMPLSRFLLQIVTEDQDLRQPEGRARAQY EAKPLLQAMPAGGLRLQIVRELADTTSTTPAEIEAICGLGSDSSRVGRFAQPRPRARRQAPTGLEQRVIQLLMCYPSLAA RLDGDARALLLDPQHSGSEALGYLVSACDGIQGEVNFAAFSEQLAQTPYADVYASARTAVLREEIEEEPATHEFDGAVHK LLAEPLEREQKALEAMVSAGTADEETKARYLWLSREVQRRRQMGWRPAAMEDS
Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]
COG id: COG0358
COG function: function code L; DNA primase (bacterial type)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Escherichia coli, GI1789447, Length=482, Percent_Identity=45.2282157676349, Blast_Score=397, Evalue=1e-112,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016136 - InterPro: IPR013264 - InterPro: IPR019475 - InterPro: IPR006295 - InterPro: IPR013173 - InterPro: IPR006171 - InterPro: IPR002694 [H]
Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]
EC number: 2.7.7.-
Molecular weight: Translated: 67580; Mature: 67580
Theoretical pI: Translated: 6.02; Mature: 6.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC CGAHGSAIGFLMEYSGQSYPDAVRELAQSVGLSVPEERNSLPPAQRAEQQARSLALTDAM CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH TRATDFYKKQLRGAPQAIQYLKGRGLTGEIAAHFGLGYAPDDWQGLETVFGSYRDDAIAG HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHC PLVEAGLLIESQEKRDADGKPRRYDRFRDRIMFPIRNTKGNVIGFGGRVMGQGEPKYLNS HHHHHHHHEECCHHCCCCCCCHHHHHHHHHEEEEEECCCCCEEEECCEEECCCCCCCCCC PETPLFSKGTELYGLFEARHAIRENGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVH CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHH VQKLMRQTDAVVFSFDGDAAGRRAARRALEACLPHVADNKTIRFLFLPAEHDPDSYVREE HHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHC GTDAFARQVQNAMPLSRFLLQIVTEDQDLRQPEGRARAQYEAKPLLQAMPAGGLRLQIVR CHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH ELADTTSTTPAEIEAICGLGSDSSRVGRFAQPRPRARRQAPTGLEQRVIQLLMCYPSLAA HHHHCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH RLDGDARALLLDPQHSGSEALGYLVSACDGIQGEVNFAAFSEQLAQTPYADVYASARTAV HCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH LREEIEEEPATHEFDGAVHKLLAEPLEREQKALEAMVSAGTADEETKARYLWLSREVQRR HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH RQMGWRPAAMEDS HHCCCCCCCCCCC >Mature Secondary Structure MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC CGAHGSAIGFLMEYSGQSYPDAVRELAQSVGLSVPEERNSLPPAQRAEQQARSLALTDAM CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH TRATDFYKKQLRGAPQAIQYLKGRGLTGEIAAHFGLGYAPDDWQGLETVFGSYRDDAIAG HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHC PLVEAGLLIESQEKRDADGKPRRYDRFRDRIMFPIRNTKGNVIGFGGRVMGQGEPKYLNS HHHHHHHHEECCHHCCCCCCCHHHHHHHHHEEEEEECCCCCEEEECCEEECCCCCCCCCC PETPLFSKGTELYGLFEARHAIRENGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVH CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHH VQKLMRQTDAVVFSFDGDAAGRRAARRALEACLPHVADNKTIRFLFLPAEHDPDSYVREE HHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHC GTDAFARQVQNAMPLSRFLLQIVTEDQDLRQPEGRARAQYEAKPLLQAMPAGGLRLQIVR CHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH ELADTTSTTPAEIEAICGLGSDSSRVGRFAQPRPRARRQAPTGLEQRVIQLLMCYPSLAA HHHHCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHH RLDGDARALLLDPQHSGSEALGYLVSACDGIQGEVNFAAFSEQLAQTPYADVYASARTAV HCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH LREEIEEEPATHEFDGAVHKLLAEPLEREQKALEAMVSAGTADEETKARYLWLSREVQRR HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH RQMGWRPAAMEDS HHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10761919 [H]