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The map label for this gene is alaXM [H]

Identifier: 94311445

GI number: 94311445

Start: 2724704

End: 2725420

Strand: Direct

Name: alaXM [H]

Synonym: Rmet_2509

Alternate gene names: 94311445

Gene position: 2724704-2725420 (Clockwise)

Preceding gene: 94311444

Following gene: 94311446

Centisome position: 69.36

GC content: 68.48

Gene sequence:

>717_bases
ATGCCCGCCACCACACAAAAGCGCTTCGACGACGACGCCTACCTGGACCACTGCCAGGCCACGGTGCTGGCCGTCAGCGA
AGCCGGCATCGAACTCGACCAGACCGTCTGCTACGCGCGTAGCGGCGGACAAGCGGGCGACACCGGCACCCTGACCACCG
CCGACGGCCGCATCGTCACACTGGCCGATACCGTTTACGCGGACGACCGCCAGCGCATCCTGCACGTCCCCGCCTCCGAC
GCCCCGGCGCTGCAGGCCGGCGACCGTGTCGAAGTGAAGATCGACTGGACCCGCCGCCACCGGCTGATGCGCCTGCATAC
GTGCCTGCATCTGCTCGGGTCGCTGATCCCGGTGCCGGTGACGGGCTGCGGCATCTCGCCTGACTCCGCGCGCATCGACT
TCGACCTGCCCGAATCGACGCTCGACAAGGCCGTGCTGACCGAACAGCTCAACGCGCTGATCAACGCGAAGACGCCGGTC
ACCATCGACCGCATCACGCCCGAAGAACTGGCCGCCCAGCCCGACCTCGTGCGCACGATCGGCGCGGCGCCGCCGGCCGG
CACATCGACGATCCGCATCATCGAGATTCCCGGCGTGGACCGTCAGCCCTGCGGCGGCACGCACGTGGCCAACACGGGCG
AGATCGGCGCGGTGGCCGTGACCAAGATCGAGAAGAAAAGCCGCACGAACCGCCGCGTGGTCGTGAACTTCGCATAA

Upstream 100 bases:

>100_bases
CGAACAAGGCGACGGCCCGCCTCTGCTGGCCGCCGACATCGAAACCCCAGCGGGCATCAAGACCCTGCGATAAACGACAC
ACACCAAAGGAGCTCCCCCG

Downstream 100 bases:

>100_bases
CAGCGCCCCCGAAACACAGAATATGGAAGCCTGGCTCACGGGGCTCTCGACAGCCCAGTTCCTTGCATTGGTCACGCTGC
TCGCGGTGGGCGCCTTCACG

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MPATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVTLADTVYADDRQRILHVPASD
APALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPV
TIDRITPEELAAQPDLVRTIGAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA

Sequences:

>Translated_238_residues
MPATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVTLADTVYADDRQRILHVPASD
APALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPV
TIDRITPEELAAQPDLVRTIGAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA
>Mature_237_residues
PATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVTLADTVYADDRQRILHVPASDA
PALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPVT
IDRITPEELAAQPDLVRTIGAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI217416402, Length=207, Percent_Identity=28.0193236714976, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI13376886, Length=207, Percent_Identity=28.0193236714976, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789048, Length=221, Percent_Identity=27.1493212669683, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 25491; Mature: 25360

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVT
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCEEHHEEEEECCCCCCCCCCEEEECCCEEEE
LADTVYADDRQRILHVPASDAPALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPV
EEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCE
TGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPVTIDRITPEELAAQPDLVRTI
ECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHH
GAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCEEEEEEC
>Mature Secondary Structure 
PATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVT
CCCCCCCCCCHHHHHHHHHEEEEEECCCCEEHHEEEEECCCCCCCCCCEEEECCCEEEE
LADTVYADDRQRILHVPASDAPALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPV
EEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCE
TGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPVTIDRITPEELAAQPDLVRTI
ECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCCCHHHHHH
GAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]