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The map label for this gene is mdh [H]

Identifier: 94311425

GI number: 94311425

Start: 2701741

End: 2702724

Strand: Direct

Name: mdh [H]

Synonym: Rmet_2489

Alternate gene names: 94311425

Gene position: 2701741-2702724 (Clockwise)

Preceding gene: 94311394

Following gene: 94311426

Centisome position: 68.78

GC content: 62.7

Gene sequence:

>984_bases
ATGGCTAAAGCCCCAATGCGCGTCGCAGTCACCGGCGCCGCTGGCCAGATCGGCTATTCCCTGCTGTTCCGCATCGCCAA
CGGCGACATGCTCGGCAAAGACCAGCCGGTCATCCTCCAACTGCTCGATCTCCCGCAAGCCCAGGCCGCCGTCAAGGGCG
TGGTGATGGAACTGGAAGACTGCGCGTTCCCGCTGCTGGCCGGCGTGGTCATTACCGACGACCCCAAGGTCGCCTTCAAG
GATGCCGACGTTGCCCTGCTGGTGGGCGCGCGTCCGCGTAGCAAGGGTATGGAACGCAAGGACCTGCTCGAAGCCAACGC
CCAGATCTTCACGGTGCAAGGCAAGGCCCTGGACGAAGTGGCCAGCCGCGACGTCAAGGTGCTGGTGGTCGGCAACCCGG
CCAACACCAACGCCTACATCGCCATGAAGTCGGCGCCGAACCTGAAGCGCGAGAACTTCACTGCCATGCTGCGCCTGGAC
CACAACCGTGCCCTGTCGCAAATCGCCGCCAAGACCGGCAAGCCGGTGTCGTCGATCGAGAAGATGTTCGTCTGGGGCAA
CCACAGCCCGACGATGTACGCCGACTACCGCTACGCCACCGTCGACGGCAAGAGCGTCAAGGACATGATCAACGATCCGG
TGTGGAACAACGACGTGTTCCTGCCGACCGTTGGCAAGCGCGGCGCTGCCATCATCGAAGCCCGTGGCCTGTCGTCGGCC
GCTTCGGCTGCCAACGCCGCCATCGACCACGTGCACGACTGGGTGCTGGGCTCGAACGGCAAGGTCGTTACGATGGGCAT
CCCGTCGAACGGTGAATACGGTATTCCGAACGATGTGATGTTCGGCTACCCGGTCACCACGGCCAACGGCAAGTACGAAA
TCGTCAAGGGTCTGGAAATTGACGCGTACAGCCAGGAGAAGATCAACATCACGCTCAAGGAACTGGAAGAAGAGCGTGCC
GGCGTGCAACACCTGCTGGGCTGA

Upstream 100 bases:

>100_bases
ACACGCGACCAGCACCCGGTGACGCGCCGGGCCGCTCCATACCCTGGAGACCCAAGCGCCACCGACCGGTTACCCCCTCT
TTTCGTTTGGAGACTTACTC

Downstream 100 bases:

>100_bases
TCCTCCGCGAGCCAACCCGCTCATGAAAGACCGGGGTTCCGCATCGATTCTCGAAAATCGGCGCGGACCCCGGTTTTTCT
TTTGGGCGCGTAAATCCCAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLD
HNRALSQIAAKTGKPVSSIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
ASAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEIDAYSQEKINITLKELEEERA
GVQHLLG

Sequences:

>Translated_327_residues
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLD
HNRALSQIAAKTGKPVSSIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
ASAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEIDAYSQEKINITLKELEEERA
GVQHLLG
>Mature_326_residues
AKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFKD
ADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLDH
NRALSQIAAKTGKPVSSIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSAA
SAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEIDAYSQEKINITLKELEEERAG
VQHLLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=328, Percent_Identity=51.8292682926829, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI89886456, Length=335, Percent_Identity=24.7761194029851, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17561064, Length=322, Percent_Identity=53.416149068323, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=260, Percent_Identity=54.6153846153846, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=47.3372781065089, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24583394, Length=320, Percent_Identity=55.9375, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35164; Mature: 35033

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELED
CCCCCEEEEEECCCCHHCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLDHNRALSQIAAKTGKPVSSIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHE
KMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
EEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCHH
ASAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECEEE
DAYSQEKINITLKELEEERAGVQHLLG
CCCCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELED
CCCCEEEEEECCCCHHCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLDHNRALSQIAAKTGKPVSSIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHE
KMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
EEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCHH
ASAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECEEE
DAYSQEKINITLKELEEERAGVQHLLG
CCCCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA