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The map label for this gene is gltA [H]

Identifier: 94311417

GI number: 94311417

Start: 2695056

End: 2696357

Strand: Reverse

Name: gltA [H]

Synonym: Rmet_2481

Alternate gene names: 94311417

Gene position: 2696357-2695056 (Counterclockwise)

Preceding gene: 94311418

Following gene: 94311416

Centisome position: 68.64

GC content: 61.98

Gene sequence:

>1302_bases
ATGACGCCGTCCGATGTGAAAGCCACGCTATCGTTTTCCGATGGTTCCCCCAGCGTTGAGCTGCCGATCTACAAGGGCAC
CGTTGGCCCGGATGTGATCGACATTCGCAAGCTGTACGGACAAACCGGCAAGTTCACCTATGACCCGGGGTTCATGTCGA
CCGCTTCGTGCAACTCCAAGATCACCTACATTGACGGTGACAAGGGCGAACTGCTGTACCGTGGCTACCCGATCGAGCAA
CTGGCACAGAAGTGCGATCACCTCGAGACCTGCTACCTGCTGCTGAAGGGCGAGCTGCCGAACGCCAAGCAGAAGGAAGA
GTTCGTGAACTCGGTGATGAACCACACGATGGTCCACGAGCAGCTCCAGTTCTTCCTGCGCGGCTTCCGCCGTGACGCGC
ACCCGATGGCCGTCCTGACGGGCCTGGTTGGCGCGATGAGCGCGTTCTACCACGACGCGATGGACATCGATGATCCGCAT
CAGCGCGAGATCTCGGCCATCCGCCTGATCGCCAAGATGCCGACCCTGGTTGCCATGGCGTACAAGTACACCATGGGTCA
GCCGTACATCTACCCGCAGAACGACCTGTCCTACTCGGGCAACTTCATGCGCATGATGTTCGCCACGCCGTGCGCCCCGT
ACACCGTGAACCCGGTGCTCGAGCGCGCGCTGGACCGCATCTTCATCCTGCACGCCGACCACGAGCAGAACGCGTCGACC
TCGACCGTGCGTCTGGCCGGTTCGTCGGGCACGAACCCGTTCGCAGCCATCGCCGCTGGCGTGGCCTGCCTGTGGGGTCC
GGCTCACGGCGGCGCGAACGAAGCCGCGCTGAAGATGCTGGAAGAAATCGGCAGCGTCGACAACATTGGCGAGTTCATCA
AGCAGGTCAAGGACAAGAACTCGGGCGTGCGCCTGATGGGCTTTGGCCACCGCGTGTACAAGAACTACGACCCGCGCGCC
AAGCTGATGCGCGAGACCTGCTATGAAGTGCTGAACGAACTGGGCCTGCACAACGACCCGCTGTTCAAGCTGGCCATGGA
GCTCGAGAAGATCGCGCTGGAAGACGAGTACTTCGTCAGCCGCAAGCTGTACCCGAACGTGGACTTCTACTCGGGTATCG
TGCAGCGCGCGCTGGGCATCCCGACCTCGCTGTTCACCTGCATCTTCGCCCTGGCACGCACGCCGGGCTGGATCTCGCAG
TGGGAAGAGATGATCACCGATCCGGAATACAAGATCGGTCGTCCGCGCCAGCTGTTCAACGGCGCCGCCGCACGCGAAGT
GCCGGATCTGGCCAAGCGCTGA

Upstream 100 bases:

>100_bases
CGGTGAGCTCGACACGCCCCCGATGCAACGGCTTATAAGCCTGCTGCGTTCGGTCTGAGTTTGGTCAACACTATTATTCA
CAGTATTTGAAGGAACCGAC

Downstream 100 bases:

>100_bases
TCTGGTTGCTGCCACGCAAGCTGAAGAACGCCCCGGTCCGCCGGGGCGTTTTTTTTGCCTTGTGTGTTGCGCCGCACTGG
CGAGAGCGGGGCAGCGCGGC

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQ
LAQKCDHLETCYLLLKGELPNAKQKEEFVNSVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPH
QREISAIRLIAKMPTLVAMAYKYTMGQPYIYPQNDLSYSGNFMRMMFATPCAPYTVNPVLERALDRIFILHADHEQNAST
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRA
KLMRETCYEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQ
WEEMITDPEYKIGRPRQLFNGAAAREVPDLAKR

Sequences:

>Translated_433_residues
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQ
LAQKCDHLETCYLLLKGELPNAKQKEEFVNSVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPH
QREISAIRLIAKMPTLVAMAYKYTMGQPYIYPQNDLSYSGNFMRMMFATPCAPYTVNPVLERALDRIFILHADHEQNAST
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRA
KLMRETCYEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQ
WEEMITDPEYKIGRPRQLFNGAAAREVPDLAKR
>Mature_432_residues
TPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQL
AQKCDHLETCYLLLKGELPNAKQKEEFVNSVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQ
REISAIRLIAKMPTLVAMAYKYTMGQPYIYPQNDLSYSGNFMRMMFATPCAPYTVNPVLERALDRIFILHADHEQNASTS
TVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRAK
LMRETCYEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQW
EEMITDPEYKIGRPRQLFNGAAAREVPDLAKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=436, Percent_Identity=26.8348623853211, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=64.4186046511628, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1786527, Length=384, Percent_Identity=27.8645833333333, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17555174, Length=412, Percent_Identity=27.1844660194175, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=342, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=374, Percent_Identity=25.4010695187166, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=370, Percent_Identity=27.2972972972973, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24640124, Length=427, Percent_Identity=26.9320843091335, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24640126, Length=425, Percent_Identity=27.0588235294118, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356863, Length=426, Percent_Identity=26.9953051643192, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48480; Mature: 48349

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSK
CCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ITYIDGDKGELLYRGYPIEQLAQKCDHLETCYLLLKGELPNAKQKEEFVNSVMNHTMVHE
EEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
QLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQREISAIRLIAKMPTLVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YKYTMGQPYIYPQNDLSYSGNFMRMMFATPCAPYTVNPVLERALDRIFILHADHEQNAST
HHHHCCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCC
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
SGVRLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHNDPLFKLAMELEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQWEEMITDPEYKIGRPRQLFN
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHC
GAAAREVPDLAKR
CCHHHCCHHHHCC
>Mature Secondary Structure 
TPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSK
CCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ITYIDGDKGELLYRGYPIEQLAQKCDHLETCYLLLKGELPNAKQKEEFVNSVMNHTMVHE
EEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
QLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQREISAIRLIAKMPTLVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YKYTMGQPYIYPQNDLSYSGNFMRMMFATPCAPYTVNPVLERALDRIFILHADHEQNAST
HHHHCCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCC
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
SGVRLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHNDPLFKLAMELEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQWEEMITDPEYKIGRPRQLFN
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHC
GAAAREVPDLAKR
CCHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]