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The map label for this gene is livK1 [H]

Identifier: 94311416

GI number: 94311416

Start: 2693263

End: 2694390

Strand: Reverse

Name: livK1 [H]

Synonym: Rmet_2480

Alternate gene names: 94311416

Gene position: 2694390-2693263 (Counterclockwise)

Preceding gene: 94311417

Following gene: 94311415

Centisome position: 68.59

GC content: 61.88

Gene sequence:

>1128_bases
ATGACGTTGTCCCGTCTCACGTCCACTGCCATGGCCGCCGTGCTTGCCACTGCCGGCCTCGCTGCCTTTACTTCGGCCAA
CGCCGAAACCATCAAGATCGCCATTGCCGGCCCGATGAGCGGCTCGGTCGCCCAGTACGGCGACATGGTCAAGGCTGGTG
CCCTGACCGCCATCGAACAGATCAATGCCGCCGGCGGCGCCAACGGCAACAAGTTCGAAGCCGTCATGATGGACGACGCT
TGCGAACCGAAGCAGGCTGTGGCCGTGGCCAACAAGATCGTGAGCCAGGGCATCCACTACGTGATTGGTCACGTGTGCTC
GGGTTCGACGATCCCGGCATCGGATATCTACGAGAACGAAGGCATCGTGATGGTCACGCCGTCGGCTACCGCGCCGCAGC
TGACCGAAACGAAGAAGCGCAACTTCATCTTCCGTACGATCGGCCGTGATGACCAGCAAGGTCCCGCAGCCGCCCAGTAC
ATCATCAACAAGGTCAAGCCGAAGAAGGTTGCCGTGCTGCACGACAAGCAGTCGTACGGCCAGGGCATCGCCAGCTCGGT
GAAGAAGGACCTCGAAGCGGCCAAGATTCCCGTGGCGGTCTTCGAAGGCATCAACGCCGGCGATTCCGACTACTCCGCTG
TCATCACCAAGCTGAAGTCGCAAGGCGTGGACTTCGTCTATTTCGGTGGCTATCACCCGGAAATGGGCCTGCTGCTGCGC
CAGGCGCGTGAGCAGGGCGTCAAGGCGACCTTCATGGGTCCCGAGGGCGTTGGCAACAAGGACGTGACGGCCATCGCCGG
TCCGGCATCGGAAGGCATGCTGGTGACGCTGCCGGCCGACTTCTCGGCCGATCCGGCCAACGCGGCCCTGGTGAAGGCTT
TCGCCGACAAGAAGCGTGACCCGAACGGTCCGTTCCAGATGCCTGCGTACGCCGCCGTGAAGATCATTGGCGATGCCATC
ACGGGCGCCAAGAGCACGGACCCGACGAAGGTTGCCGCTTACATGCACAAGAACGCGTTCAACACGCCGATCGGCAAGGT
TGAATATGACGCGAAGGGCGACCTGAAGTCGTTCAAGTTCGTCGTCTACACGTGGCACAAGGACGCCACGAAGACCGCCG
CCAACTAA

Upstream 100 bases:

>100_bases
CGTTCGCCACGTCAGGCGGCGCATTCCCGCGGGTGTTGTTCGATTGCGCCGGTCATGCCGACTCTGCCCGGATGACCAAT
ACATAATCGAGGAGCTCCTG

Downstream 100 bases:

>100_bases
ATCCCGGCGCCATTCTGGCGAGACGCCCCGCTCCCGACCCGGTGAGCGGGGCGTTTTGGTATCGGTTCGATGTTGATAGA
TCGCCACATGACGGCGGTCA

Product: leucine ABC transporter subunit substrate-binding protein LivK

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDA
CEPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQY
IINKVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLR
QAREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIGDAI
TGAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKFVVYTWHKDATKTAAN

Sequences:

>Translated_375_residues
MTLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDA
CEPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQY
IINKVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLR
QAREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIGDAI
TGAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKFVVYTWHKDATKTAAN
>Mature_374_residues
TLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDAC
EPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQYI
INKVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLRQ
AREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIGDAIT
GAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKFVVYTWHKDATKTAAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=372, Percent_Identity=51.8817204301075, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI48994941, Length=363, Percent_Identity=51.5151515151515, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39315; Mature: 39184

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
INAAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENE
HHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHCCCCC
GIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQYIINKVKPKKVAVLHDKQSYG
CEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
QGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLR
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
QAREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRD
HHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCC
PNGPFQMPAYAAVKIIGDAITGAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKF
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCEEE
VVYTWHKDATKTAAN
EEEEECCCCHHCCCC
>Mature Secondary Structure 
TLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
INAAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENE
HHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHCCCCC
GIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQYIINKVKPKKVAVLHDKQSYG
CEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
QGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLR
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
QAREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRD
HHHHCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCC
PNGPFQMPAYAAVKIIGDAITGAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKF
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCEEE
VVYTWHKDATKTAAN
EEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]