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The map label for this gene is leuC [H]

Identifier: 94311411

GI number: 94311411

Start: 2687720

End: 2689129

Strand: Reverse

Name: leuC [H]

Synonym: Rmet_2475

Alternate gene names: 94311411

Gene position: 2689129-2687720 (Counterclockwise)

Preceding gene: 94311412

Following gene: 94311410

Centisome position: 68.46

GC content: 64.89

Gene sequence:

>1410_bases
ATGGCCAAGACGCTCTACGACAAACTCTGGGATGACCACACCGTCCACGTCGAGGAAGACGGCACCACGCTGCTCTACAT
CGATCGCCACCTGCTTCACGAAGTGACCAGCCCGCAGGCGTTCGAAGGACTAAAGCTGGCGGAGCGTCCGGTCTGGCGCA
TCAGCGCCAACCTGGCCGTGTCGGATCACAACGTGCCGACCACCGACCGCTCGCAAGGCATCGCCGACCCGATCTCGAAG
CTCCAGGTGGACACGCTCGACCTGAACTGCGATGCCTATGGCATCACCCAGTTCAAGATGAACGATCACCGCCAGGGCAT
CGTTCACGTGATCGGGCCGGAGCAGGGCGCCACGCTGCCCGGCATGACCGTGGTCTGCGGTGACTCGCACACCAGCACGC
ACGGCGCGTTCGGTGCGCTGGCCCACGGCATCGGCACGTCGGAAGTGGAGCATGTGCTGGCCACCCAGACGTTGCTGGGC
AAGAAGGCCAAGAACATGCTGATCAAGGTGGAAGGCAAGCTGCCCCGCGGCTGCACCGCCAAGGACATCGTGCTGGCCGT
CATCGGCAAGATCGGCACGGCGGGCGGCACGGGCTACACGATGGAGTTCGCCGGCTCGGCCATTCGCGACCTGACGATGG
AAGGCCGCATGACCGTCTGCAACATGGCAATCGAAGCCGGCGCGCGTGCCGGCCTGGTGGCCGTGGACGATGTCACGCTC
GAATACGTCAAGAACCGTCCATTCGCGCCGCAAGGCGTGGAGTGGGACCAGGCCGTGGCGTACTGGCGCACGCTGCATTC
GGATGCCGGCGCCCACTTCGACAAGGTTGTGGAGCTGCGTGCCGAAGAAGTCCGTCCGCAGGTGACCTGGGGCACGTCGC
CCGAAATGGTCATCAGCATCGAGGACCGCGTTCCGGATCCGGAGAAGGAAAAGGACTCGAACAAGCGCAACGCGATGGAG
CGCGCGCTCGAGTACATGGGCCTGCAGCCGAACGTGCCGATGGAGTCGATCAACATCGACAAGGTGTTCATCGGGTCCTG
CACCAACAGCCGCATCGAAGATATGCGTGCCGCCGCGTGGGTCGTTCAGAAGCTGGGCCGCAAGGTCGCGTCGAACGTGA
AGCTGGCGATGGTGGTGCCGGGATCGGGTCTGGTGAAGGAACAGGCCGAGCGCGAAGGCCTGGACAAGATCTTCAAGGCT
GCCGGCTTCGAATGGCGCGAGCCGGGGTGCTCGATGTGCCTGGCGATGAACGCTGACCGCCTGGATCCGGGCGAGCGCTG
CGCGTCGACGTCGAATCGCAACTTCGAAGGCCGTCAGGGCGCCGGTGGCCGCACGCACCTGGTGAGCCCGGCGATGGCTG
CCGCGGCCGCGATCGAGGGGCACTTCGTCGATATTCGCAAGCTCGGTTGA

Upstream 100 bases:

>100_bases
CCGCGAAGGCATCCGGAAAACAATTCCCCTGCAAACCAATGCGCGGACACGCGCCGCTGCTGCCCAGGATGGGCAGTACG
GACGGTTCGCCACTCGCATC

Downstream 100 bases:

>100_bases
GCCTGCGCCGATCGGCATTGAGACATATGAAAAAGTTCGTGATCACGCTGCTGGCATGTCTGGGCATGCTGCAAATCGCC
GGCTGCAACACCATGGCCGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSQGIADPISK
LQVDTLDLNCDAYGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLG
KKAKNMLIKVEGKLPRGCTAKDIVLAVIGKIGTAGGTGYTMEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDDVTL
EYVKNRPFAPQGVEWDQAVAYWRTLHSDAGAHFDKVVELRAEEVRPQVTWGTSPEMVISIEDRVPDPEKEKDSNKRNAME
RALEYMGLQPNVPMESINIDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Sequences:

>Translated_469_residues
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSQGIADPISK
LQVDTLDLNCDAYGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLG
KKAKNMLIKVEGKLPRGCTAKDIVLAVIGKIGTAGGTGYTMEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDDVTL
EYVKNRPFAPQGVEWDQAVAYWRTLHSDAGAHFDKVVELRAEEVRPQVTWGTSPEMVISIEDRVPDPEKEKDSNKRNAME
RALEYMGLQPNVPMESINIDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
>Mature_468_residues
AKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAVSDHNVPTTDRSQGIADPISKL
QVDTLDLNCDAYGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGK
KAKNMLIKVEGKLPRGCTAKDIVLAVIGKIGTAGGTGYTMEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDDVTLE
YVKNRPFAPQGVEWDQAVAYWRTLHSDAGAHFDKVVELRAEEVRPQVTWGTSPEMVISIEDRVPDPEKEKDSNKRNAMER
ALEYMGLQPNVPMESINIDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKIFKAA
GFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=364, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI41352693, Length=373, Percent_Identity=27.3458445040214, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8659555, Length=407, Percent_Identity=25.3071253071253, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=65.3846153846154, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1787531, Length=369, Percent_Identity=28.9972899728997, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=367, Percent_Identity=30.5177111716621, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=379, Percent_Identity=29.8153034300792, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=29.8360655737705, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=406, Percent_Identity=26.3546798029557, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.4067796610169, Blast_Score=611, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6323335, Length=389, Percent_Identity=28.0205655526992, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=26.7605633802817, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=363, Percent_Identity=27.8236914600551, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI281365315, Length=395, Percent_Identity=27.8481012658228, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17864292, Length=395, Percent_Identity=27.8481012658228, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=27.8481012658228, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28571643, Length=472, Percent_Identity=27.7542372881356, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=27.2, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137564, Length=383, Percent_Identity=25.065274151436, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50993; Mature: 50861

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV
CCCHHHHHHCCCCEEEEECCCCEEEEECHHHHHHCCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSQGIADPISKLQVDTLDLNCDAYGITQFKMNDHRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEEEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGKKAKNMLIKVEGKLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
KDIVLAVIGKIGTAGGTGYTMEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDDVTL
HHHHHHHHHHHCCCCCCCEEHHHCCHHHHHHHCCCCHHEEHHHHHCCCCCCEEEECHHHH
EYVKNRPFAPQGVEWDQAVAYWRTLHSDAGAHFDKVVELRAEEVRPQVTWGTSPEMVISI
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
EDRVPDPEKEKDSNKRNAMERALEYMGLQPNVPMESINIDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKLAERPVWRISANLAV
CCHHHHHHCCCCEEEEECCCCEEEEECHHHHHHCCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSQGIADPISKLQVDTLDLNCDAYGITQFKMNDHRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEEEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGKKAKNMLIKVEGKLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
KDIVLAVIGKIGTAGGTGYTMEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDDVTL
HHHHHHHHHHHCCCCCCCEEHHHCCHHHHHHHCCCCHHEEHHHHHCCCCCCEEEECHHHH
EYVKNRPFAPQGVEWDQAVAYWRTLHSDAGAHFDKVVELRAEEVRPQVTWGTSPEMVISI
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
EDRVPDPEKEKDSNKRNAMERALEYMGLQPNVPMESINIDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCHHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA