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The map label for this gene is leuB [H]

Identifier: 94311408

GI number: 94311408

Start: 2685804

End: 2686871

Strand: Reverse

Name: leuB [H]

Synonym: Rmet_2472

Alternate gene names: 94311408

Gene position: 2686871-2685804 (Counterclockwise)

Preceding gene: 94311409

Following gene: 94311407

Centisome position: 68.4

GC content: 63.76

Gene sequence:

>1068_bases
ATGAAGATTGCAGTTCTGCCGGGCGATGGCATCGGCCCCGAGATCGTTGCTGAAGCCGTCAAGGTGCTCAACGCACTGGA
CGAGAAATTCGAGATGGAAACCGCGCCGGTCGGCGGCGCTGGCTACGAGGCCGAAGGCCATCCGCTGCCAGACAACACGC
TGAAGCTGGCCAAGGAAGCCGACGCCATCCTGTTTGGCGCCGTGGGCGACTGGAAGTACGACAGCCTGGATCGCCCGCTG
CGTCCCGAGCAGGCCATTCTCGGCCTGCGCAAGCATCTGCAGCTGTTCGCCAACTTCCGTCCGGCTATTTGCTACCCGGA
ATTGACCGGCGCATCGAGCCTGAAGCCCGAACTGGTGGCTGGCCTGGATATCCTGATCGTGCGCGAGCTGAACGGCGACA
TCTACTTCGGCCAGCCGCGCGGCGTGCGCGAGGCGCCGGATGGCCTGTTCAAGGGTGCCCGCGAAGGCTTCGACACGATG
CGCTACAGCGAGCCGGAAGTCCGCCGCATCGCGCATGTGGCGTTCCAGGCCGCTGCAAAGCGCGGCAAGAAGCTGTGCAG
CGTCGATAAGGCCAACGTGCTCGAGACTTTCCAGTTCTGGAAAGACATCGTGATCGACGTCAGCAAGGAGTACCCGGACG
TCGAGCTGTCGCACATGTACGTGGACAACGCCGCGATGCAGCTGGTCAAGGCGCCGAAGAGCTTCGACGTGATCGTCACC
GGCAACATGTTCGGTGACATCCTGTCCGACGAGGCTGCGATGCTGACCGGTTCGATCGGCATGCTGCCGTCGGCGTCGCT
CGATGCCAACAACAAGGGTCTGTATGAGCCGTCGCACGGCTCGGCGCCTGACATCGCCGGCAAGGGCGTGGCGAATCCGC
TGGCCACGATCCTCTCCGCCGCGATGATGCTGCGCTATTCGCTGAACCGCGCCGAACAGGCTGACCGCATCGAGAACGCT
GTCAAGAAGGTGCTGGCCCAAGGCTATCGCACGGCTGACATCGTGACGCCGGGCTGCAAGCAGGTTGGCACGCGCGAGAT
GGGCGAGGCGGTGCTGGCGGCACTGTAA

Upstream 100 bases:

>100_bases
GGACAAGATCAAGGCATACGAGGCCGAGCGCCTCACGAAGATGCCGTGGCTGGGCAACCGCGTGGTTGGCTGAGGCAAGA
CAAACTACAGGATCAGAAAG

Downstream 100 bases:

>100_bases
GGCCAGGCGCCGAGTGGTTTGCTTCCCCTTTCCCGCAAGGCGGGAGCGGGGCAGCGGGGGACGGCCGGGCGGTGCAGGAT
GCGAATTCGCAATTTGTTGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPDNTLKLAKEADAILFGAVGDWKYDSLDRPL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEVRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNRAEQADRIENA
VKKVLAQGYRTADIVTPGCKQVGTREMGEAVLAAL

Sequences:

>Translated_355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPDNTLKLAKEADAILFGAVGDWKYDSLDRPL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEVRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNRAEQADRIENA
VKKVLAQGYRTADIVTPGCKQVGTREMGEAVLAAL
>Mature_355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPDNTLKLAKEADAILFGAVGDWKYDSLDRPL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEVRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNRAEQADRIENA
VKKVLAQGYRTADIVTPGCKQVGTREMGEAVLAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=332, Percent_Identity=31.6265060240964, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI28178816, Length=371, Percent_Identity=28.0323450134771, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178821, Length=335, Percent_Identity=28.0597014925373, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4758582, Length=323, Percent_Identity=27.8637770897833, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=27.8637770897833, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI28178819, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=52.112676056338, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI1788101, Length=349, Percent_Identity=34.3839541547278, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=26.75, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=338, Percent_Identity=31.6568047337278, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=30.4093567251462, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=29.5522388059701, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=29.2899408284024, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.5753424657534, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=36.0795454545455, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=341, Percent_Identity=31.0850439882698, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324709, Length=367, Percent_Identity=29.4277929155313, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24643268, Length=346, Percent_Identity=32.9479768786127, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24643270, Length=346, Percent_Identity=32.9479768786127, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=29.7687861271676, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161078635, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078633, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=336, Percent_Identity=26.4880952380952, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=26.6467065868263, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.6118980169972, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.6118980169972, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38315; Mature: 38315

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPDNTLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHC
DAILFGAVGDWKYDSLDRPLRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHH
GLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTMRYSEPEVRRIAHVAFQAAAK
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
HCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEHHHHHHHHHHCCCCCEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMMLRYSLNRAEQADRIENAVKKVLAQGYRTADIVTPGCKQVGTREMGEAVLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPDNTLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHC
DAILFGAVGDWKYDSLDRPLRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA
CEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHH
GLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTMRYSEPEVRRIAHVAFQAAAK
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
HCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEHHHHHHHHHHCCCCCEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMMLRYSLNRAEQADRIENAVKKVLAQGYRTADIVTPGCKQVGTREMGEAVLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]