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The map label for this gene is trpA

Identifier: 94311401

GI number: 94311401

Start: 2676407

End: 2677204

Strand: Reverse

Name: trpA

Synonym: Rmet_2465

Alternate gene names: 94311401

Gene position: 2677204-2676407 (Counterclockwise)

Preceding gene: 94311402

Following gene: 94311400

Centisome position: 68.16

GC content: 67.79

Gene sequence:

>798_bases
ATGTCCCGTATCCAGAAGACTTTCGCGGCACTGGCCGCGCAGCAGAAGAAGGGCCTGATTCCGTTCATCACCGCCGGCGA
TCCGGCTCCCGCGTTGACCGTCGACCTGATGCACGCCCTCGTGGCTGGCGGCGCCGACGTGATCGAGCTTGGCGTGCCGT
TTTCGGACCCGATGGCCGACGGCCCCGTGATTCAGCGCGCCTCCGAGCGCGCGCTGGCCCAGGGCGTTTCGCTGACGCAG
GTGCTGGCCTGGGTGACCGAGTTCCGCAAGACGAACGCGACCACGCCGGTAGTGCTGATGGGCTACGCCAACCCGATCGA
GCGCATGGGTGAGGAAACCTTCGCCAAGGCGGCCAGCGCGGCCGGCGTGGACGGCGTGCTCGTGGTCGACTACCCGCCCG
AAGAGTGCGAGTCTTTCGCCGCGCTGATGCGCGCGAACCAGATGGACCCGATCTTCCTGCTGGCGCCGACCTCGACCGAC
GACCGTATCGCCGCGGTGGCGAAGGTGGCCAGTGGCTATCTGTACTACGTGTCGCTCACGGGCGTGACCGGCTCGGCCAC
GCTCGACCTCGAGAGCGTGGCTGCGCGTCTGCCGCTGATCAAGCAGCACGCGAACCTGCCCGTGGGCGTGGGTTTCGGTA
TCCGTGATGCGCAAACCGCACGTGCCATCGGCAGCGTGGCCGACGCCGTGGTGATTGGCAGCCGCCTGGTGCAGTTGCTT
GAGGATGCGCCGCGCGAGAAGGCCGTGGACTCCCTGCGTGCCTTTATCGCCGACATCCGCCAGGCACTGGACGCCTGA

Upstream 100 bases:

>100_bases
AGCCGCAAGCGTCCGATGTCGCCGATACCGCCGACGCCGCCAATGACGATGCCAATGAAGCGGCATCGCCCGTGCAACGC
CTTATCTGATTGCCACTGAC

Downstream 100 bases:

>100_bases
TTTCTGGTCCAACGCTTGACGTACCGCTGAGAAAATCGCGCCATTTATGATTCTTGCTGCACAAATGGCGCGTTTTGCTT
CGGTCCGATGGCCCGGGCAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSRIQKTFAALAAQQKKGLIPFITAGDPAPALTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALAQGVSLTQ
VLAWVTEFRKTNATTPVVLMGYANPIERMGEETFAKAASAAGVDGVLVVDYPPEECESFAALMRANQMDPIFLLAPTSTD
DRIAAVAKVASGYLYYVSLTGVTGSATLDLESVAARLPLIKQHANLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
EDAPREKAVDSLRAFIADIRQALDA

Sequences:

>Translated_265_residues
MSRIQKTFAALAAQQKKGLIPFITAGDPAPALTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALAQGVSLTQ
VLAWVTEFRKTNATTPVVLMGYANPIERMGEETFAKAASAAGVDGVLVVDYPPEECESFAALMRANQMDPIFLLAPTSTD
DRIAAVAKVASGYLYYVSLTGVTGSATLDLESVAARLPLIKQHANLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
EDAPREKAVDSLRAFIADIRQALDA
>Mature_264_residues
SRIQKTFAALAAQQKKGLIPFITAGDPAPALTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALAQGVSLTQV
LAWVTEFRKTNATTPVVLMGYANPIERMGEETFAKAASAAGVDGVLVVDYPPEECESFAALMRANQMDPIFLLAPTSTDD
RIAAVAKVASGYLYYVSLTGVTGSATLDLESVAARLPLIKQHANLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLLE
DAPREKAVDSLRAFIADIRQALDA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=269, Percent_Identity=35.6877323420074, Blast_Score=152, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=260, Percent_Identity=40, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_RALME (Q1LKI4)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_584611.1
- ProteinModelPortal:   Q1LKI4
- SMR:   Q1LKI4
- STRING:   Q1LKI4
- GeneID:   4039288
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_2465
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q1LKI4
- ProtClustDB:   PRK13111
- BioCyc:   RMET266264:RMET_2465-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27803; Mature: 27672

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIQKTFAALAAQQKKGLIPFITAGDPAPALTVDLMHALVAGGADVIELGVPFSDPMAD
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALAQGVSLTQVLAWVTEFRKTNATTPVVLMGYANPIERMGEETFAKAASA
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
AGVDGVLVVDYPPEECESFAALMRANQMDPIFLLAPTSTDDRIAAVAKVASGYLYYVSLT
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEE
GVTGSATLDLESVAARLPLIKQHANLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
CCCCCCEECHHHHHHHCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAPREKAVDSLRAFIADIRQALDA
HHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIQKTFAALAAQQKKGLIPFITAGDPAPALTVDLMHALVAGGADVIELGVPFSDPMAD
CHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALAQGVSLTQVLAWVTEFRKTNATTPVVLMGYANPIERMGEETFAKAASA
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
AGVDGVLVVDYPPEECESFAALMRANQMDPIFLLAPTSTDDRIAAVAKVASGYLYYVSLT
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEEEE
GVTGSATLDLESVAARLPLIKQHANLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
CCCCCCEECHHHHHHHCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAPREKAVDSLRAFIADIRQALDA
HHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA