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The map label for this gene is folC [H]

Identifier: 94311399

GI number: 94311399

Start: 2673952

End: 2675262

Strand: Reverse

Name: folC [H]

Synonym: Rmet_2463

Alternate gene names: 94311399

Gene position: 2675262-2673952 (Counterclockwise)

Preceding gene: 94311400

Following gene: 94311398

Centisome position: 68.11

GC content: 64.0

Gene sequence:

>1311_bases
ATGCCCATCTTCCACAATCTCCCCGACTGGCTCGCACATCTTGAAACCGCTCACCCCGTGGGCATCGACATGGGCCTGAG
CCGCATCACGCGGGTCAAGGAAGCGCTGGGCCTGCGTATCGACGCCGTGGTCTTCACGGTGGGCGGTACCAACGGCAAGG
GATCCACCTGCGCGATCCTGGAGCGCATCCTGCTCGAAGCGGGCTACAAGGTGGGCCTGCATACCTCGCCGCACCTGATC
CAGTTCAACGAGCGTGCCCGCCTCAATGGCGAGCAGGCGACGGACGCCCAGCTTCTGCCGCATTTCGAGGCCGTCGAGCG
CGCACGCACGAGCTTTGCCGATCCGGTCAGCCTGACGTATTTCGAGTTCACGACGCTGGCGATCATCCACATGTTTGCCG
AAGCCGGACTCGACGCGATCATTCTGGAAGTCGGTCTCGGTGGCCGCCTGGATGCGGTCAATGTGATTGATACGGACTGC
GCGATTATCACGAGTGTGGATATCGACCACACCCAGTACCTTGGCGACACACGTGAGAAGATCGGCTACGAGAAGGCCGG
CATCTTCCGCCCGCACGTGCCGGCGATCGTCGGCGATCCCTTGCCACCGCAGTCGGTGATCGATCACGCCGAGGCGATCG
GCGCCGAACTCTGGCTCGTGGGACGCGATTTCCGCCACCAGGCCGCGCAGGGCCAGGAACGCCAGCAATGGGACTGGGCC
AGCCGCGACCGCAAGCTGAATGGCCTGGGCTACCCGGCATTGCGTGGCGCCAACCAGCTGCTCAACGCATCTGCGGCGCT
GGCCGCGCTGCAGGCCATGCGTTCGCGCCTGCCTGTGAGCGCGCAGGAAGTGCGCAACGGCCTGGCGTTCGTGGAGTTGC
CGGGACGGTTCCAGGTGCTGCCGGGCCGACCGGCCGTGATCCTCGATGTCGCGCACAACCCCCATGCCGCAGCGACGCTT
GGACAGAACCTTGAGAATATGGGGTTCTTCCGTTACACCTATGCGGTGTTCGGCGCCATGCACGACAAGGACATCGCCGG
CGTGCTGCAACACGTGGCCGACAAGGTGGACCACTGGTGCCTGTGCGACCTGCCGACCGACCGGGCCGCTACTGCCGCGG
AATTGCGCGAGAAGCTTGAGGCAACTGGCTTCCGTGAGGGCCCGGACAACACCGTTGCATGTTTTTCCAGCCCCGAACTT
GCGTATCGCGATGCTGTCGAGCGCGCTACCGAGAATGATAGAATCCTGGTCTTCGGGTCGTTCTACACAGTGGCCGGCGT
GATGGCATATCGTGCCACGCAGGCCAACTGA

Upstream 100 bases:

>100_bases
GCCGGCTGACGCGGTAGCCTGACGCGGCAAGCAAATTTGCAGGACAATGGCGCGGGTGCCGAATACCCGCGCCATTTTGC
TTTTGTAGTTCTGAATCCGT

Downstream 100 bases:

>100_bases
GCCGGAACGGCATCGCACCGAACACCATACTTTGGGCATCTATGGGCCTGCTTTCGCTGTTCTCGTCCCGTAAGGGCACT
GACCCGGCACCCGAACGCGG

Product: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAILERILLEAGYKVGLHTSPHLI
QFNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTYFEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDC
AIITSVDIDHTQYLGDTREKIGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWA
SRDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATL
GQNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWCLCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPEL
AYRDAVERATENDRILVFGSFYTVAGVMAYRATQAN

Sequences:

>Translated_436_residues
MPIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAILERILLEAGYKVGLHTSPHLI
QFNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTYFEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDC
AIITSVDIDHTQYLGDTREKIGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWA
SRDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATL
GQNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWCLCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPEL
AYRDAVERATENDRILVFGSFYTVAGVMAYRATQAN
>Mature_435_residues
PIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAILERILLEAGYKVGLHTSPHLIQ
FNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTYFEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDCA
IITSVDIDHTQYLGDTREKIGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWAS
RDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATLG
QNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWCLCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPELA
YRDAVERATENDRILVFGSFYTVAGVMAYRATQAN

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=377, Percent_Identity=30.2387267904509, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI66932984, Length=377, Percent_Identity=30.2387267904509, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1788654, Length=425, Percent_Identity=43.0588235294118, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17553150, Length=293, Percent_Identity=29.3515358361775, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=293, Percent_Identity=29.3515358361775, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=293, Percent_Identity=29.3515358361775, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=396, Percent_Identity=29.040404040404, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324815, Length=172, Percent_Identity=38.3720930232558, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24641571, Length=159, Percent_Identity=35.8490566037736, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24581568, Length=151, Percent_Identity=36.4238410596026, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47675; Mature: 47543

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAIL
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHH
ERILLEAGYKVGLHTSPHLIQFNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTY
HHHHHHCCCEEECCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHH
FEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDCAIITSVDIDHTQYLGDTREK
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCCCHHHHHCCHHHH
IGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWA
CCCHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHCCHH
SRDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCEEEC
PGRPAVILDVAHNPHAAATLGQNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWC
CCCCEEEEEECCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEE
LCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPELAYRDAVERATENDRILVFGS
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEEC
FYTVAGVMAYRATQAN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAIL
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHH
ERILLEAGYKVGLHTSPHLIQFNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTY
HHHHHHCCCEEECCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHH
FEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDCAIITSVDIDHTQYLGDTREK
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCCCHHHHHCCHHHH
IGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWA
CCCHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCHHHCCHH
SRDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCEEEC
PGRPAVILDVAHNPHAAATLGQNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWC
CCCCEEEEEECCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEE
LCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPELAYRDAVERATENDRILVFGS
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEEC
FYTVAGVMAYRATQAN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]