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The map label for this gene is yfiN [H]

Identifier: 94311183

GI number: 94311183

Start: 2458956

End: 2460236

Strand: Direct

Name: yfiN [H]

Synonym: Rmet_2245

Alternate gene names: 94311183

Gene position: 2458956-2460236 (Clockwise)

Preceding gene: 94311182

Following gene: 94311184

Centisome position: 62.6

GC content: 68.93

Gene sequence:

>1281_bases
ATGAACTGGCCCGGCCGCCCCCCCGCCCGCGACGACGCCGTGCCACAGCGCCCGACGCTGTACCGCGCGCTGCGGCGCAT
CCACCTTGGCGTGGCGCTGATCGCCGTGTTCACGGTCGGCATCTCGCTGACCGCGCTCGGGCTGACCGCGCTGCGCGTCT
ATGCGGATCACAACCTCCGGCTGGTCGCGCGCTCGATCGCCTATACGGTGGAAGCGCCGGTCGTGTTCGGCGACGCCGCC
GCCGCGGCCGATGCGCTTTCCGTGATCGCCGCATCCGAGGACGTGACGCGGGTCATCGTGTACGACCGCGACCACCAGCC
GATGACCGTGTGGCAGCGCCCCGGTCACGAGCAATTCCCCGCGCTGCGCCGGGTGGCCGGCGACCTGCTGATATCGGGCG
CCGTCGAGCAGCCAATCCTTCACGAAGCCCAGCAGGTAGGCAGCGTGCGTGTGGCAGGCGATCCGGTGGCGCTCCTGCAC
TTCCTGCTGCAAGGCACGCTGGGCCTGGGAGCCTGTCTGCTGTTGACGGCGCTGGTGGCGCACTACATGTCCCGCCGAAT
GCTCAGGGATGTCGTCAATCCCCTGCGCAACCTGATGCGCGTGGCCCACGCGGTGCGTTCCGAACGCGCGTTTGGACAGC
GAATGCCACCGGCGCGGATCGAGGAACTGAATGCGCTGTCCGAAGACTTCAACGTCCTGCTGGACGAACTCGAGGCCTGG
CAAAACCAGCTTCAGCACGAGAACCGTTCGCTGGCGCACCGCGCCAGCCATGACAGCCTGACAGGCTTGCTCAACCGCGC
CGCCTTCACTGAAGCGCTCGATCTCGCCGTGCGTACTGCGGCGGCGCGCAATCAATATGCCGCCGTGCTCTATCTCGACA
GCGACGACTTCAAGGGTATCAACGACCGCTATGGCCACGCGGCGGGCGACGCCGTGCTCGTCGCCGTCGGTCAACGCCTG
CGCGGCTGCCTGCGCGAAGGCGACAGCGTGGCGCGACTGGGCGGCGACGAGTTCGCCGTGCTGGTCAACGCCCTCCGCGC
GCCCGAGGACGCGGTTCGCATCGCCGAGCACATCCTCAACGCCATGGCCACCCCTGTGTGTCTGGCCGATGGCACCACCA
TCTTCGCGCCGCTCAGCATCGGCCTGGCGGTCTACCCGCTGCATGCCCGCAACGGCACGGCGCTGCTCCATTCCGCCGAT
GCCGCGATGTACGGGGCCAAGCGCCAGTCCGGCGCCACCTGGCACAGCGCGGAGTCCGCGCCACCGGAGCCACATCTCTA
G

Upstream 100 bases:

>100_bases
ATCAAGTTTCGTTCCGGATCAATCTCGATGCCATCGCGCGCAGCGGGATTCATATCCACCCCGGTGTGCTCCAGCTCGGC
CGCCGCAGGACGCCCGCATC

Downstream 100 bases:

>100_bases
AGGCCGACCATGAGAGCGCCCAGCAGACCCCGCCGCGCTTTCCTGCTGACCACACTGGCCGGTCTGGGCGCGCTGGCGCT
GTCCGGCTGCGCCAGCCCCG

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLRLVARSIAYTVEAPVVFGDAA
AAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFPALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLH
FLLQGTLGLGACLLLTALVAHYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAW
QNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGINDRYGHAAGDAVLVAVGQRL
RGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILNAMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSAD
AAMYGAKRQSGATWHSAESAPPEPHL

Sequences:

>Translated_426_residues
MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLRLVARSIAYTVEAPVVFGDAA
AAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFPALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLH
FLLQGTLGLGACLLLTALVAHYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAW
QNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGINDRYGHAAGDAVLVAVGQRL
RGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILNAMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSAD
AAMYGAKRQSGATWHSAESAPPEPHL
>Mature_426_residues
MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLRLVARSIAYTVEAPVVFGDAA
AAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFPALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLH
FLLQGTLGLGACLLLTALVAHYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAW
QNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGINDRYGHAAGDAVLVAVGQRL
RGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILNAMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSAD
AAMYGAKRQSGATWHSAESAPPEPHL

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=401, Percent_Identity=34.4139650872818, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=35.2272727272727, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787541, Length=154, Percent_Identity=38.961038961039, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=35.8490566037736, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=40.625, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=35.4430379746835, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=31.7365269461078, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=32.3170731707317, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=34.5454545454545, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.7560975609756, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081974, Length=102, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46102; Mature: 46102

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LVARSIAYTVEAPVVFGDAAAAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFP
HHHHHHHHHEECCEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHH
ALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLHFLLQGTLGLGACLLLTALVA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
NDRYGHAAGDAVLVAVGQRLRGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILN
CHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSADAAMYGAKRQSGATWHSAESA
HHCCCEEEECCCEEEEHHHCCEEEEEEECCCCCEEEEECHHHHHCCCCCCCCCCCCCCCC
PPEPHL
CCCCCC
>Mature Secondary Structure
MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LVARSIAYTVEAPVVFGDAAAAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFP
HHHHHHHHHEECCEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHH
ALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLHFLLQGTLGLGACLLLTALVA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
NDRYGHAAGDAVLVAVGQRLRGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILN
CHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSADAAMYGAKRQSGATWHSAESA
HHCCCEEEECCCEEEEHHHCCEEEEEEECCCCCEEEEECHHHHHCCCCCCCCCCCCCCCC
PPEPHL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]